We narrowed to 4,131 results for: p2a

1. P-Ef1a-RPL22-3XHA-P2A-EGFP-T2A-Puro

   Plasmid

   #170317

   Purpose

   Expresses HA and EGFP tagged RPL22, puromycin resistance

   Depositor

   Hemali Phatnani

   Article

   Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

   Insert

   RPL22 (RPL22 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX-4T1 human PP2A B56α full length

   Plasmid

   #132635

   Purpose

   expresses human PP2A B56α full length in E. coli

   Depositor

   Wenqing Xu

   Article

   Sangodkar et al J Clin Invest. 2017 Jun 1;127(6):2081-20

   Insert

   PP2A B56α
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA

   Plasmid

   #50943

   Purpose

   Bicistronic vector expressing mRuby2 and GCaMP6f from a single open reading frame.

   Depositor

   Tobias Rose, Mark Huebener, Tobias Bonhoeffer

   Article

   Rose et al Science. 2016 Jun 10;352(6291):1319-22.

   Insert

   mRuby2-P2A-GCaMP6f
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn1

   Available since

   March 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. P-Ef1a-RPL22-3XHA-P2A-mCherry-T2A-Puro

   Plasmid

   #170320

   Purpose

   Expresses HA and mCherry tagged RPL22, puromycin resistance

   Depositor

   Hemali Phatnani

   Article

   Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

   Insert

   RPL22 (RPL22 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR-U6-sgTel-CMV-puro-P2A-mCherry-TRF1

   Plasmid

   #169449

   Purpose

   expression of TRF1-mcherrry and sgRNA targeting telomeres

   Depositor

   Stanley Qi

   Article

   Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

   Insert

   mCherry-TRF1
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-SynI-CreOn-FlpOff-mNaChBac-P2A-EGFP

   Plasmid

   #176280

   Purpose

   Viral vector for co-expression of NaChBac and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.

   Depositor

   Martin Riccomagno

   Article

   Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

   Insert

   mNaChBac-P2A-EGFP
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human Synapsin I

   Available since

   Jan. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. BC1209-pHR-dSV40-dCas9-14xGFP11-P2A-BFP

   Plasmid

   #164038

   Purpose

   Expression of dCas9-14xGFP11 in mammalian cells

   Depositor

   Baohui Chen

   Article

   Chen et al Nat Commun. 2018 Nov 29;9(1):5065. doi:

   Insert

   dCas9-14xGFP11
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A and H840A in Cas9

   Promoter

   dSV40

   Available since

   Jan. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLN539 (FuGW-G8p-antiPD1(HC)-P2A-antiPD1(LC)-1Pe)

   Plasmid

   #105200

   Purpose

   Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of anti-PD1 Ab transcripts containing miR1-Bs(Pe)

   Depositor

   Timothy Lu

   Article

   Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

   Insert

   G8p-antiPD1(HC)-P2A-antiPD1(LC)
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR-H13LTat-Thy1.2-P2A-GFP-T2A-Nef

   Plasmid

   #126553

   Purpose

   Replication incompetent HIV with H13L Tat and GFP-t2a-Thy1.2 reporter

   Depositor

   Jonathan Karn

   Article

   Dobrowolski et al MBio. 2019 Mar 26;10(2). pii: mBio.00337

   Insert

   HIV-1 vector pNL4-3
   

   Use

   Lentiviral

   Tags

   Thy1.2 and GFP

   Expression

   Mutation

   Promoter

   HIV LTR

   Available since

   July 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. Lenti-EFS-FLTID-GSQ-RBXN-P2A-BLAST

    Plasmid

    #208041

    Purpose

    Enables constitutive expression of TurboID alone; RBXN represents a EcoR1-BsiW1-Xba1-Not1 polylinker; selection with blasticidin

    Depositor

    James Sutherland, Rosa Barrio

    Article

    Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

    Insert

    TurboID
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC19-TgKI-MCS-p2A-eGFP-MCS-polyA

    Plasmid

    #174028

    Purpose

    This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

    Depositor

    Michel Bagnat

    Article

    Levic et al Development. 2021 Sep 8. pii: 272090. do

    Insert

    p2A-eGFP
    

    Use

    Zebrafish knock-in tagging

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. mCherry-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax

    Plasmid

    #173872

    Purpose

    Mammalian expression of mCherry-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax (opto-Cdc42)

    Depositor

    Takao Nakata

    Article

    Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

    Insert

    mCherry-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax
    

    Use

    Tags

    C-terminal CAAX-box and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. P-Ef1a-RPL22-3XHA-P2A-mCherry-T2A-Neo

    Plasmid

    #170321

    Purpose

    Expresses HA and mCherry tagged RPL22, neomycin resistance

    Depositor

    Hemali Phatnani

    Article

    Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

    Insert

    RPL22 (RPL22 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-T7-TadCBEd-SpCas9(LWKYQS)-P2A-EGFP (BKS1014)

    Plasmid

    #223127

    Purpose

    pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with LWKYQS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadA-CDd-SpCas9(LWKYQS)-2xUGI-P2A-EGFP
    

    Use

    CRISPR

    Tags

    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

    Expression

    Mammalian

    Mutation

    TadA-CDd mutations; nSpCas9(D10A/LWKYQS)=D1135L, …

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-hSyn1-EGFP-P2A-EGFPf-WPRE-HGHpA

    Plasmid

    #74513

    Purpose

    AAV vector that use human synapsin-1 promoter to drive the expression of EGFP and membrane-targeted EGFPf linked by self-cleaving P2A peptide.

    Depositor

    Guoping Feng

    Article

    Zhang et al J Neurosci. 2016 Feb 17;36(7):2247-60. d

    Insert

    EGFP-p2A-EGFP-f
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA-CRY2-mCh-superPLDx100-P2A-CIBN-CAAX

    Plasmid

    #188994

    Purpose

    A plasmid for dual expression of CRY2-mCh-superPLDx100 (an engineered PLD mutant with 100 times higher activity than the wild-type) and plasma membrane-tagged CIBN

    Depositor

    Jeremy Baskin

    Article

    Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

    Insert

    PLD
    

    Use

    Tags

    CAAX, CIBN, CRY2, P2A, and mCherry

    Expression

    Mammalian

    Mutation

    K57R, A59V, K109R, P245A, V264I, G328S, G381V, G4…

    Promoter

    CMV

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. lenti U6-sgRNA-acRNA SYN-dCas9-P2A-EGFP

    Plasmid

    #159086

    Purpose

    Expresses dCas9-P2A-EGFP driven by human SYN promoter and empty CRISPR Display sgRNA/accessory RNA from U6 promoter.

    Depositor

    Jeremy Day

    Article

    Carullo et al Nucleic Acids Res. 2020 Aug 18. pii: 589

    Insert

    empty crRNA-acRNA backbone, dCas9-P2A-EGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    EGFP and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    hSYN, U6

    Available since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAM-CAGGS-FLEX-H2B-3XHA-P2A-N2c(G)

    Plasmid

    #73477

    Purpose

    Encodes the G protein to enable transsynaptic RAV infection

    Depositor

    Thomas Jessell

    Article

    Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

    Insert

    H2B-3XHA-P2A-N2c(G)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3

    Plasmid

    #108912

    Purpose

    Cation channelrhodopsin ChroME targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

    Depositor

    Hillel Adesnik

    Article

    Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

    Has Service

    AAV9

    Insert

    ChroME-ST
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGL3-sgRab18.N-Cas9-T2A-mCherry-P2A-Puro

    Plasmid

    #129418

    Purpose

    Encoding Cas9 and sgRAB18.N for CRISPR/Cas9 mediated HDR tagging of endogenous human RAB18 N-terminus

    Depositor

    Elina Ikonen

    Article

    Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

    Insert

    SpCas9 and sgRAB18.N (RAB18 Human)
    

    Use

    CRISPR

    Tags

    T2A-mCherry-P2A-Puro

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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