Addgene

We narrowed to 5,794 results for: RIK

Showing: 3481 - 3520 of 5794 results

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NHR1-Flag pBacPAK8-3

Plasmid

#17323

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2007 Dec 12. ():.

Insert

Neuralized-like (Neurl1a Mouse)

Use

Tags

FLAG

Expression

Insect

Mutation

Promoter

Available since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
pAAV FLEX Synaptophysin GFP

Plasmid

#137188

Purpose

Cre-dependent expression of synaptophysin-GFP

Depositor

Matthew Nolan

Article

Surmeli et al Neuron. 2016 Nov 23;92(4):929. doi: 10.1

Insert

Synaptophysin GFP (Syp Mouse)

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pre_IV_CS8_7569_v1

Plasmid

#126865

Purpose

7886 bases long scaffold with Zn-dependent self-cleaving DNAzyme cassettes, from compatibility group 8, made by method IV, yields linear 7569 b long scaffolds after Zn incubation

Depositor

Hendrik Dietz

Article

Engelhardt et al ACS Nano. 2019 Apr 22. doi: 10.1021/acsn

Insert

IV_CS8_7569_v1

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 5, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Egr1

Plasmid

#11729

Depositor

Eileen Adamson

Article

Yu et al Mol Cell. 2004 Jul 2. 15(1):83-94.

Insert

Egr-1 (Egr1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

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Flag-Gsdmd

Plasmid

#80950

Purpose

Expresses Gsdmd in mammalian cells

Depositor

Judy Lieberman

Article

Liu et al Nature. 2016 Jul 6;535(7610):153-8. doi:

Insert

Gsdmd (Gsdmd Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

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mCh-Climp63

Plasmid

#136293

Purpose

expression of Climp63

Depositor

Gia Voeltz

Article

Shibata et al J Biol Chem. 2008 Jul 4. 283(27):18892-9

Insert

Cytoskeletal-linking protein 63 (Ckap4 Mouse)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgSlc18a2

Plasmid

#124849

Purpose

Mutagenesis of Slc18a2

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Slc18a2 (Slc18a2 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEXfrt-SaCas9-U6-sgSlc18a2

Plasmid

#124862

Purpose

Mutagenesis of Slc18a2

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Slc18a2 (Slc18a2 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pcdna nesprin TS

Plasmid

#68127

Purpose

nesprin tension sensor

Depositor

Daniel Conway

Article

Arsenovic et al Biophys J. 2016 Jan 5;110(1):34-43. doi:

Insert

mouse nesprin mini 2G (Syne2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pcdna nesprin HL

Plasmid

#68128

Purpose

nesprin headless control

Depositor

Daniel Conway

Article

Arsenovic et al Biophys J. 2016 Jan 5;110(1):34-43. doi:

Insert

nesprin-2 (Syne2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

deleted actin-binding domain

Promoter

CMV

Available since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pEF6-myc-TBC1D15

Plasmid

#79148

Purpose

mammalian expression of TBC1D15

Depositor

Aimee Edinger

Article

Peralta et al J Biol Chem. 2010 May 28. 285(22):16814-

Insert

TBC1D15 (Tbc1d15 Mouse)

Use

Tags

His, Myc, and V5

Expression

Mammalian

Mutation

S616N (please see depositor comment below)

Promoter

EF-1alpha

Available since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TDsmURFP-IRES-HO-1

Plasmid

#80346

Purpose

Expresses Tandem Dimer smURFP and Heme Oxygenase-1 in mammalian cells.

Depositor

Erik Rodriguez, Roger Tsien

Article

Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

Inserts

Tandem Dimer smURFP
HO-1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and IRES

Available since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-CaMKKbeta

Plasmid

#32453

Depositor

Anthony Means

Article

Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)

Use

Tags

FLAG and GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 23, 2012

Availability

Academic Institutions and Nonprofits only

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Stat3 pcDNA3

Plasmid

#8706

Purpose

See Depositor Comments below for variant information.

Depositor

Jim Darnell

Article

Zhong et al Proc Natl Acad Sci U S A 1994 May 24;91(

Insert

Stat3 (Stat3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

* see comments

Promoter

Available since

Sept. 26, 2005

Availability

Academic Institutions and Nonprofits only

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TAL2451

Plasmid

#36840

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

HMGA2-TALEN_Right (Hmga2 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Expression

Mutation

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
Stat3-C Flag pRc/CMV

Plasmid

#8722

Depositor

Jim Darnell

Article

Bromberg et al Cell 1999 Aug 6;98(3):295-303.

Insert

Stat3-C (Stat3 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

A662C and N664C. Residues changed to Cys renders…

Promoter

Available since

Sept. 26, 2005

Availability

Academic Institutions and Nonprofits only

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pPB_TRE3G_scFV-sfGFP-Setd2CD

Plasmid

#235579

Purpose

Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Setd2 fused with scFV to programme H3K36me3

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

Setd2 (Setd2 Mouse)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 29, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-AcGFP1-ORP9-PH

Plasmid

#214266

Purpose

Mammalian expression of green fluorescent PI4P reporter AcGFP1-ORP9-PH

Depositor

Shinya Tsukiji

Article

Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

Insert

AcGFP1-ORP9(1-111) (Osbpl9 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry_EHMT1_full length

Plasmid

#228810

Purpose

Expression of mouse EHMT1

Depositor

Shravanti Rampalli

Article

Biligiri et al PLOS Biology 22(11): e3002871.

Insert

EHMT1 (Ehmt1 Mouse)

Use

Tags

mCherry

Expression

Mammalian

Mutation

See Depositor Comments

Promoter

Available since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-HCMVgH (TB40/E)

Plasmid

#136446

Purpose

Mammalian expression plasmid for HCMV glycoprotein H (TB40/E strain), full-length

Depositor

Erik Procko

Article

Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

Insert

glycoprotein H (UL75 Human betaherpesvirus 5)

Use

Tags

Expression

Mammalian

Mutation

Codon-optimized for human cell expression.

Promoter

CMV

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
PGK 4xMito-mEmerald

Plasmid

#200430

Purpose

Mammalian expression of fluorescently-tagged marker for mitochondria

Depositor

Erika Holzbaur

Article

Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

Insert

4x cytochrome c oxidase subunit VIII mitochondrial targeting sequence

Use

Tags

mEmerald

Expression

Mammalian

Mutation

4x mito-targeting sequence

Promoter

PGK

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-calpainsensor

Plasmid

#36182

Purpose

detection of activity of ubiquitous calpains

Depositor

Isabelle Richard

Article

Stockholm et al J Mol Biol. 2005 Feb 11;346(1):215-22. E

Insert

alpha-Fodrin cleavage site (Sptan1 Mouse)

Use

Tags

eCFP and eYFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only

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3XFlagNICD1

Plasmid

#20183

Depositor

Raphael Kopan

Article

Ong et al J Biol Chem. 2006 Feb 24. 281(8):5106-19

Insert

Notch 1 Intracellular Domain (Notch1 Mouse)

Use

Tags

3XFLAG

Expression

Mammalian

Mutation

Contains murine Notch 1 Intracellular Domain (Val…

Promoter

CMV

Available since

Dec. 19, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti6/UbC/mSlc7a1

Plasmid

#17224

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2007 Nov 30. 131(5):861-72.

Insert

Slc7a1 (Slc7a1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pBetaActin-mNeonGreen-CAMSAP3

Plasmid

#191322

Purpose

Expresses the fluorescently labeled microtubule minus end marker CAMSAP3 to measure microtubule retrograde flow

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

mNeonGreen-CAMSAP3 (Camsap3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pNCS mOrange

Plasmid

#91764

Purpose

Bacterial expression of a fluorescent protein, monomeric Orange (mOrange)

Depositor

Erik Rodriguez, Roger Tsien

Article

Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

Insert

monomeric Orange

Use

Tags

Hexa-Histidine tag, Xpress epitope for detection …

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 9, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti EF1a GFP-mCherry-mPlin1

Plasmid

#189005

Purpose

Fluorescent reportor for lipophagy

Depositor

Atsushi Hoshino

Article

Minami et al Nat Commun. 2023 Jul 13;14(1):4084. doi:

Insert

GFP, mChery, Plin1 (Plin1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-SynB

Plasmid

#174861

Purpose

Expresses mouse syncytin B in mammalian cells

Depositor

Feng Zhang

Article

Segel et al Science. 2021 Aug 20;373(6557):882-889.

Insert

syncytin B (Synb Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pT3-EF1a-NICD1

Plasmid

#46047

Depositor

Xin Chen

Article

Fan et al J Clin Invest. 2012 Aug 1;122(8):2911-5.

Insert

Notch1 (C-term) (Notch1 Mouse)

Use

Tags

Myc tag

Expression

Mammalian

Mutation

Cytoplasmic domain initiating at amino acid 1753

Promoter

EF1a

Available since

July 2, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-TDsmURFP-IRES-eGFP

Plasmid

#80344

Purpose

Expresses Tandem Dimer smURFP and eGFP in mammalian cells.

Depositor

Erik Rodriguez, Roger Tsien

Article

Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

Inserts

Tandem Dimer smURFP
eGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and IRES

Available since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-f:PGC1

Plasmid

#1026

Depositor

Bruce Spiegelman

Article

Monsalve et al Mol Cell 2000 Aug;6(2):307-16.

Insert

PPAR gamma coactivator 1 alpha (Ppargc1a Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
mVenus-IQ6 WT

Plasmid

#198201

Purpose

FRET acceptor: IQ6 domain of Moysin Va tagged with N-terminal mVenus

Depositor

Christian Wahl-Schott

Article

Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

Insert

MYO5A IQ domain (Myo5a Mouse)

Use

Tags

mVenus

Expression

Mammalian

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEJS614_pTetR-P2A-BFPnls/sgNS

Plasmid

#108650

Purpose

Non-specific Spy sgRNA under U6 promoter

Depositor

Erik Sontheimer

Article

Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

Inserts

Non-specific sgRNA
TetR-P2A-BFP

Use

CRISPR and Lentiviral

Tags

No

Expression

Mammalian

Mutation

Promoter

U6 and hPGK

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pNCS mStrawberry

Plasmid

#91768

Purpose

Bacterial expression of a fluorescent protein, monomeric Strawberry (mStrawberry)

Depositor

Erik Rodriguez, Roger Tsien

Article

Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

Insert

monomeric Strawberry

Use

Tags

Hexa-Histidine tag, Xpress epitope for detection …

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 8, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pT3-EF1aH NICD1

Plasmid

#86500

Purpose

Mammalian expression of Myc tagged NICD1

Depositor

Xin Chen

Article

Fan et al J Clin Invest. 2012 Aug 1;122(8):2911-5.

Insert

Notch1 (C-term) (Notch1 Mouse)

Use

Tags

Myc

Expression

Mammalian

Mutation

Cytoplasmic domain only (AA 1753-2531)

Promoter

EF1a

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV-synaptophysin-Lime

Plasmid

#201016

Purpose

Expresses Lime in synaptic vesicle lumen by fusion to synaptophysin

Depositor

Jaime de Juan-Sanz

Article

Shen et al ACS Sens. 2023 Aug 25;8(8):3014-3022. do

Insert

Synaptophysin-Lime (Syp Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-CCR5

Plasmid

#98943

Purpose

Mammalian expression plasmid for untagged human CCR5

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

CCR5 (CCR5 Human)

Use

Tags

Expression

Mammalian

Mutation

Full-length, wildtype

Promoter

CMV

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS PKC theta

Plasmid

#8426

Depositor

Frederic Mushinski

Article

Mischak et al FEBS Lett 1993 Jul 12;326(1-3):51-5.

Insert

Mouse Protein Kinase C theta (Prkcq Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 22, 2005

Availability

Academic Institutions and Nonprofits only
pET28-SaCas9

Plasmid

#178901

Purpose

SaCas9 (Cas9 from Staphylococcus aureus)

Depositor

Hendrik Milting

Article

Flottmann et al STAR Protoc. 2022 Apr 5;3(2):101276. doi

Insert

SaCas9

Use

Tags

His6-Tag, bipartite nuclear localization signal f…

Expression

Bacterial

Mutation

Promoter

Available since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAT-WT-Myc

Plasmid

#177717

Purpose

ER protein alpha-1-antitrypsin (WT), myc tagged

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

alpha-1-antitrypsin (SERPINA1 Human)

Use

Tags

Myc

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

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