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pVP16-tev-PRT1-C195A
Plasmid#83408Purposebacterial expression vector pVP16 containing a mutant variant of Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studiesDepositorInsertPROTEOLYSIS1 (PRT1) (PRT1 Mustard Weed, E. coli)
UseTagsHis8, MBP, and tev cleavage siteExpressionBacterialMutationC195A substitutionPromoterAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVP16-tev-PRT1-C29A
Plasmid#83407Purposebacterial expression vector pVP16 containing a mutant variant of Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studiesDepositorInsertPROTEOLYSIS1 (PRT1) (PRT1 Mustard Weed, E. coli)
UseTagsHis8, MBP, and tev cleavage siteExpressionBacterialMutationC29A substitutionPromoterAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVP16-tev-PRT1-V28A
Plasmid#83406Purposebacterial expression vector pVP16 containing a mutant variant of Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studiesDepositorInsertPROTEOLYSIS1 (PRT1) (PRT1 Mustard Weed, E. coli)
UseTagsHis8, MBP, and tev cleavage siteExpressionBacterialMutationV28A substitutionPromoterAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458_HHEX_2
Plasmid#72355PurposeEncodes gRNA for 3' target of human HHEX along with Cas9 with 2A GFPDepositorInsertHHEX (HHEX Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHAC111-LST8 (pSW75)
Plasmid#39211DepositorInsertLST8 (LST8 Budding Yeast)
UseTagsExpressionYeastMutationPromoterLST8Available sinceNov. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSR1037
Plasmid#208736PurposeCharacterization plasmid for PSarA1 (sfgfp expressed from PSarA1 on the S. aureus pC194 replicon)DepositorInsertPSarA1
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSR1058
Plasmid#208743PurposeCharacterization plasmid for PHbs (sfgfp expressed from PHbs on the S. aureus pC194 replicon)DepositorInsertPHbs
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPHbsAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pERB264
Plasmid#67764PurposeTags peroxisomes with GFP and HalotagDepositorInsertPEX3-GFP-Halo
UseTagsGFP and HalotagExpressionMammalianMutationPromoterAvailable sinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSR1038
Plasmid#208737PurposeCharacterization plasmid for PCap (sfgfp expressed from PCap on the S. aureus pC194 replicon)DepositorInsertPCap
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPCapAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK7-HA-S6K1-F5A-E389-R3A
Plasmid#8991DepositorInsertS6K1 (Rps6kb1 Rat)
UseTagsHAExpressionMammalianMutationF5A, T389E, R3A (C-terminal RSPRR motif to ASPAA)PromoterAvailable sinceNov. 14, 2005AvailabilityAcademic Institutions and Nonprofits only -
pGL4-23-NegCtrl
Plasmid#163904PurposeNegative control vector for luciferase reporter assaysDepositorInsertLuciferase
UseLuciferaseTagsExpressionMutationPromoterminimalAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pERB254
Plasmid#67762PurposeTags mitochondria with Halotag and GFPDepositorInsertHalo-GFP-Mito
UseTagsGFP and HalotagExpressionMammalianMutationPromoterAvailable sinceDec. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMCSG17-NleL
Plasmid#66716PurposeExpresses NleL (170-782) in E. coliDepositorInsertNleL (NEWENTRY )
UseTagsGST-TEVExpressionBacterialMutationPromoterT7Available sinceJuly 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
p5E-QUAS (L4-R1)
Plasmid#61374Purpose5' Gateway Entry Vector Containing QUASDepositorInsertQUAS
UseTagsExpressionMutationPromoterAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX-HiGlpG
Plasmid#158538PurposeBacterial expression of rhomboid protease HiGlpGDepositorInsertHiGlpG
UseTagsGST tagExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDO23A
Plasmid#27367DepositorInsertpUC/ori, bla
UseGateway donor vectorTagsExpressionBacterialMutationN/APromoterAvailable sinceMay 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRK7-HA-S6K1-F5A-E389-deltaCT
Plasmid#8990DepositorInsertS6K1 (Rps6kb1 Rat)
UseTagsHAExpressionMammalianMutationF5A, T389E, stop codon at amino acid 402PromoterAvailable sinceSept. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
pCxcl1-Firefly
Plasmid#163896PurposeLuciferase reporter for mouse Cxcl1DepositorInsertLuciferase
UseLuciferaseTagsExpressionMutationPromoterCxcl1Available sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only
