We narrowed to 6,509 results for: cas9 expression plasmid

1. pAAV-CMV-dSa VP64 Hbb

   Plasmid

   #99693

   Purpose

   Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Hbb promoter, vector allows for activation of mouse Hbb, can be packaged and delivered as AAV

   Depositor

   George Church

   Article

   Vora et al bioRxiv 2018. 298620

   Insert

   dCas9 and gRNA targeting Hbb (gRNA: GGGGTAAGGGGAGCAAGGTC) (Hbb Synthetic, Mouse)
   

   Use

   AAV and CRISPR

   Tags

   VP64

   Expression

   Mutation

   dead Cas9

   Promoter

   Available since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. MSP469

   Plasmid

   #65771

   Purpose

   Human expression vector for SpCas9 VQR variant: CMV-T7-humanSpCas9(D1135V/R1335Q/T1337R)-NLS-3xFLAG (VQR variant)

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/R1335Q/T1337R)-NLS-3XFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   D1135V, R1335Q, and T1337R mutations in Cas9

   Promoter

   CMV & T7

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. MSP680

   Plasmid

   #65772

   Purpose

   Human expression vector for SpCas9 EQR variant: CMV-T7-humanSpCas9(D1135E/R1335Q/T1337R)-NLS-3xFLAG (EQR variant)

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E/R1335Q/T1337R)-NLS-3XFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   D1135E, R1335Q, and T1337R mutations in Cas9

   Promoter

   CMV & T7

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. px459 VQR

   Plasmid

   #101715

   Purpose

   sgRNA/Cas9 expression plasmid with Cas9 VQR mutations (NGA PAM)

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 VQR
   

   Use

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   VQR (D1135V, R1335Q and T1337R)

   Promoter

   Available since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. AZ64_pE.DonorCLYBL.TS

   Plasmid

   #199228

   Purpose

   Donor plasmid with CLYBL target sites for ITPN or HMEJ knock-in at the human CLYBL safe harbor locus

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.

   Insert

   Expression unit for EGFP reporter
   

   Use

   Gene targeting donor plasmid

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human PGK1 gene promoter

   Available since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-sgRNA-Lib

   Plasmid

   #53121

   Purpose

   sgRNA expression construction in a 3rd generation lentiviral backbone

   Depositor

   Wensheng Wei

   Article

   Zhou et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCASB

   Plasmid

   #190175

   Purpose

   Plasmid for yeast expression of Cas9; contains a golden gate cloning site to additionally express a gRNA for a target sequence

   Depositor

   Jessica Tyler

   Article

   Aguilar et al Methods Protoc. 2022 Oct 4;5(5):79. doi:

   Insert

   sgRNA cloning site
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Added 5'-agagacc-3' upstream and 5'…

   Promoter

   Available since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pORFE1001

   Plasmid

   #112079

   Purpose

   Overexpression of the AtCAS9 in plant under 2x35S promoter

   Depositor

   Christian Chevalier

   Insert

   AtCAS9
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Plant codon optimized Cas9 S. pyogenes

   Promoter

   2x35S

   Available since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. 4xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7 probasin_mTQ2_FlpO

   Plasmid

   #68356

   Purpose

   -The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex7 and SMAD4 ex2. are expressed by the U6 promoter

   Depositor

   Jannik Elverløv-Jakobsen

   Inserts

   4xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7
   FlpO recombinase
   

   Use

   CRISPR

   Tags

   mTurquoise2 (BFP)

   Expression

   Mammalian

   Mutation

   Promoter

   Synthetic Probasin ARRx2 and U6

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. HCP5

    Plasmid

    #166107

    Purpose

    This plasmid encodes a Cas9 protein as well as a sgRNAs that targets the C-terminus of Cyr1.

    Depositor

    Matthias Heinemann

    Insert

    Cyr1CT-sgRNA (CYR1 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. PX552

    Plasmid

    #60958

    Purpose

    pAAV-U6sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). AAV plasmid for sgRNA cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei.

    Depositor

    Feng Zhang

    Article

    Swiech et al Nat Biotechnol. 2014 Oct 19. doi: 10.103

    Inserts

    U6_(SpaI)_sgRNA
    Syn_EGFP-KASH
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin and U6

    Available since

    Nov. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCSD_2xNES-FRB-ZFPTS2-3xFLAG-2xNLS

    Plasmid

    #107303

    Purpose

    Expresses ZFP-VEGFA-TS2 fused to FRB in mammalian cells

    Depositor

    Scot Wolfe

    Insert

    ZFP_VEGFA-TS2
    

    Use

    CRISPR

    Tags

    2xNES, 3x Flag, 2xNLS, and FRB

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCSD_2xNES-FRB-ZFPTS3-3xFLAG-2xNLS

    Plasmid

    #107304

    Purpose

    Expresses ZFP-VEGFA-TS3 fused to FRB in mammalian cells

    Depositor

    Scot Wolfe

    Insert

    ZFP_VEGFA-TS3
    

    Use

    CRISPR

    Tags

    2xNES, 3x Flag, 2xNLS, and FRB

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. psBIG1a

    Plasmid

    #229957

    Purpose

    bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. psBIG1b

    Plasmid

    #229958

    Purpose

    bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. psBIG2abc

    Plasmid

    #229963

    Purpose

    bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. psBIG2ab

    Plasmid

    #229962

    Purpose

    bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. psBIG2abcd

    Plasmid

    #229964

    Purpose

    bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. psBIG2abcde

    Plasmid

    #229965

    Purpose

    bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. psBIG1d

    Plasmid

    #229960

    Purpose

    bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge