We narrowed to 18,413 results for: multi

1. pZS1[RbsR-L,TreR-L]

   Plasmid

   #60766

   Purpose

   Contains PIq driving expression RbsR-L, and PI driving expression of TreR-L.

   Depositor

   Matthew Bennett

   Article

   Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

   Inserts

   RbsR-L
   TreR-L
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   LacI/GalR repressor chimera. LacI DBD with RbsR L…

   Available Since

   April 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. DBJS-p8.35

   Plasmid

   #246898

   Purpose

   Used for multi-site piggybac transposon insertion of luciferase reporter

   Depositor

   David Bartel

   Insert

   CNOT10::ecDHFR::NanoLuc::MS2v5 + Blasticidin resistance piggybac
   

   Expression

   Mammalian

   Available Since

   Oct. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. DBJS-p8.38

   Plasmid

   #246899

   Purpose

   Used for multi-site piggybac transposon insertion of luciferase reporter

   Depositor

   David Bartel

   Insert

   DDIT4L::ecDHFR::NanoLuc::MS2v5 + Blasticidin resistance piggybac
   

   Expression

   Mammalian

   Available Since

   Oct. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. DBJS-p8.32

   Plasmid

   #246897

   Purpose

   Used for multi-site piggybac transposon insertion of luciferase reporter

   Depositor

   David Bartel

   Insert

   RB1::ecDHFR::NanoLuc::MS2v5 + Blasticidin resistance piggybac
   

   Expression

   Mammalian

   Available Since

   Oct. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. cfSYFP2-N

   Plasmid

   #110631

   Purpose

   Cys-free yellow fluorescent protein for extracellular imaging. SYFP2 variant (C48S/C70M). Molecular brightness is almost comparable to SYFP2.

   Depositor

   Ikuo Wada

   Insert

   cfSYFP2-N
   

   Tags

   Multi-cloning sites

   Expression

   Mammalian

   Mutation

   Cys48Ser and Cys70/Met

   Available Since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPV475

   Plasmid

   #42928

   Depositor

   James Lok

   Insert

   attR2::Ss-era-1 terminator (598 bp)::attL3
   

   Use

   Multi-site gateway donor plasmid

   Available Since

   March 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEvol-pAzFRS.2.t1

   Plasmid

   #73546

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG

   Depositor

   Farren Isaacs

   Article

   Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

   Inserts

   pAzFRS.2.t1
   pAzFRS.2.t1
   

   Expression

   Bacterial

   Available Since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEvol-pAcFRS.2.t1

   Plasmid

   #73544

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG

   Depositor

   Farren Isaacs

   Article

   Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

   Inserts

   pAcFRS.2.t1
   pAcFRS.2.t1
   

   Expression

   Bacterial

   Available Since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDL52 (clone76)

   Plasmid

   #188579

   Purpose

   High-yield production of coenzyme F420 in E. coli

   Depositor

   Gerald Lackner

   Article

   Last et al Metab Eng. 2022 Jul 18. pii: S1096-7176(

   Inserts

   ribA
   ribD
   yigB
   fbiC
   cofC
   cofD
   cofI
   cofE
   

   Expression

   Bacterial

   Mutation

   Codon optimization, synthetic ribosome binding si…

   Promoter

   T7 variants

   Available Since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pORTMAGE311B

    Plasmid

    #120418

    Purpose

    Expresses Lambda Beta and a dominant negative MutL E32K allele, controlled by XylS-Pm expression system for high precision and efficient MAGE experiments at 37°C. RSF1010 Ori; Broad host-range; KanR

    Depositor

    Csaba Pál

    Article

    Szili et al bioRxiv (2018)

    Inserts

    MutL E32K
    Lambda Beta
    XylS
    

    Expression

    Bacterial

    Mutation

    E32K mutation conferring dominant mutator phenoty…

    Promoter

    Pm

    Available Since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. 24xMoonTag-kif18b-STOP-24xSunTag-linker-24xPP7 (3’UTR translation reporter)

    Plasmid

    #128605

    Purpose

    Expresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene, STOP codon, 24xgcn4 peptide array (SunTag peptide array), linker and 24 repeats of PP7 hairpins.

    Depositor

    Marvin Tanenbaum

    Article

    Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

    Inserts

    24xMoonTag
    kinesin family member 18b (KIF18B Human)
    24xSunTag
    

    Expression

    Mammalian

    Promoter

    Pcmv

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEvol-pAzFRS.1.t1

    Plasmid

    #73547

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of p-azido-l-phenylalanine, into proteins in E coli in response to the amber codon, TAG

    Depositor

    Farren Isaacs

    Article

    Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

    Inserts

    pAzFRS.1.t1
    pAzFRS.1.t1
    

    Expression

    Bacterial

    Available Since

    March 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEMT-TPE2A-Mef2c-Tdtomato-Gata4-Tbx5

    Plasmid

    #111818

    Purpose

    Quadcistronic construct: Co-express four genes by three 2A peptides -- T2A, P2A and E2A

    Depositor

    Li Qian

    Article

    Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

    Inserts

    Mef2c (Mef2c Mouse)
    Tdtomato
    Gata4 (Gata4 Mouse)
    Tbx5
    

    Use

    Cloning intermediate

    Available Since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDL52 (clone45)

    Plasmid

    #188578

    Purpose

    High-yield production of coenzyme F420 in E. coli

    Depositor

    Gerald Lackner

    Article

    Last et al Metab Eng. 2022 Jul 18. pii: S1096-7176(

    Inserts

    ribA
    ribD
    yigB
    fbiC
    cofC
    cofD
    cofI
    cofE
    

    Expression

    Bacterial

    Mutation

    Codon optimization, synthetic ribosome binding si…

    Promoter

    T7 variants

    Available Since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEvol-pAcFRS.1.t1

    Plasmid

    #73545

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG

    Depositor

    Farren Isaacs

    Article

    Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

    Inserts

    pAcFRS.1.t1
    pAcFRS.1.t1
    

    Expression

    Bacterial

    Available Since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pETM30-C9ORF78_F8A

    Plasmid

    #193369

    Purpose

    Expression of C9ORF78 F8A variant in E.coli with N-terminal His6-GST-tag which is cleavable with TEV protease

    Depositor

    Markus Wahl

    Article

    Bergfort et al Nat Commun. 2022 Mar 3;13(1):1132. doi:

    Insert

    C9ORF78_F8A (C9orf78 Human)
    

    Tags

    His6-GST-TEV

    Expression

    Bacterial

    Mutation

    F8A, the mutation is located in the binding inter…

    Promoter

    T7

    Available Since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. T7pr-His6-MBP-TEV-dCas9-BirA*

    Plasmid

    #159994

    Purpose

    Bacterial expression of dCas9-BirA*

    Depositor

    Jacob Corn

    Article

    Wang et al Mol Cell. 2020 Jul 16;79(2):221-233.e5.

    Insert

    dCas9-BirA*
    

    Tags

    6X-His, MBP, 3X-FLAG, NLS

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    T7

    Available Since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. LSB-hsa-miR-92a-1-5p

    Plasmid

    #103750

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-92a-1-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-92a-1-5p target (MIR92A1 Human)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHD-sfGFP-ScarlessDsRed-GMR-eya(shRNA)

    Plasmid

    #240228

    Purpose

    Parental donor plasmid for scarless sfGFP-fusion knock-in fly lines. Red fluorescent fly eye marker to select for knock-in events, small fly eye marker to select against backbone integration.

    Depositor

    Norbert Perrimon, Justin Bosch

    Article

    Bosch et al bioRxiv 2025.07.09.659702; doi: https://

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Insect

    Available Since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHD-T2A-Gal4-3xP3DsRed-GMR-eya(shRNA)

    Plasmid

    #240229

    Purpose

    Parental donor plasmid for T2A-Gal4 knock-in fly lines. Red fluorescent fly eye marker to select for knock-in events, small fly eye marker to select against backbone integration.

    Depositor

    Norbert Perrimon, Justin Bosch

    Article

    Bosch et al bioRxiv 2025.07.09.659702; doi: https://

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Insect

    Available Since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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