We narrowed to 4,131 results for: p2a

1. mCherry-NES-SspB-Tiam-DHPH-P2A-iLIDcaax

   Plasmid

   #173871

   Purpose

   Mammalian expression of mCherry-NES-SspB-Tiam-DHPH-P2A-iLIDcaax (opto-Rac1)

   Depositor

   Takao Nakata

   Article

   Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

   Insert

   mCherry-NES-SspB-Tiam-DHPH-P2A-iLIDcaax
   

   Use

   Tags

   C-terminal CAAX-box and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-EF1α-DIO-GtACR2-P2A-Voltron2ST-W3SL

   Plasmid

   #217676

   Purpose

   AAV-mediated, Cre-dependent co-expression of GtACR2 and soma-targeted Voltron2 (ORCHID) for assessing inhibitory receptor driving force through voltage imaging with concurrent activation of GtACR2.

   Depositor

   Joseph Raimondo

   Insert

   GtACR2-P2A-Voltron2ST
   

   Use

   AAV

   Tags

   Soma targeting sequence (Kv2.1) on Voltron2 gene …

   Expression

   Mammalian

   Mutation

   Promoter

   EF‐1α

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. MF87_PB-EF1a-TET3G-P2A-ERT2-Gal4-BGHpA

   Plasmid

   #176630

   Purpose

   Integrated in CHO-K1 to make parental polyclonal lines for all other MultiFate cell lines

   Depositor

   Michael Elowitz

   Article

   Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.

   Insert

   EF1a-TET3G-P2A-ERT2-Gal4-BGHpA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-CAG-SV40NLSf-mScarlet-P2A-hLuc-WPRE

   Plasmid

   #203539

   Purpose

   AAV reporter genome plasmid

   Depositor

   Ben Deverman

   Article

   Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

   Insert

   NLS-mScarlet-2A-mScarlet
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-SynI-CreOn-FlpOff-mNaChBacMUT-P2A-EGFP

   Plasmid

   #176281

   Purpose

   Viral vector for co-expression of non-functional NaChBac and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.

   Depositor

   Martin Riccomagno

   Article

   Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

   Insert

   mNaChBacMUT-P2A-EGFP
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   E191K

   Promoter

   human Synapsin I

   Available since

   Jan. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. SECURE BE3(R33A/K34A)-P2A-EGFP (pJUL1410)

   Plasmid

   #123615

   Purpose

   CAG promoter expression plasmid for rAPOBEC1(R33A/K34A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A/K34A).

   Depositor

   Keith Joung

   Article

   Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

   Insert

   BE3(R33A/K34A)-P2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R33A/K34A in rAPOBEC1, D10A in SpCas9

   Promoter

   CAG

   Available since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAF0362 Ef1a-Cp36_attP-Puro-p2a-mCherry donor

   Plasmid

   #193469

   Purpose

   Cp36 Donor with promoter for mcherry and puro expression

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Cp36_attP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-SynI-CreOn-FlpOff-Kir2.1-P2A-EGFP

   Plasmid

   #176278

   Purpose

   Viral vector for co-expression of Kir2.1 and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.

   Depositor

   Martin Riccomagno

   Article

   Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

   Insert

   Kir2.1-P2A-EGFP (Kcnj2 Mouse, Synthetic)
   

   Use

   AAV and Cre/Lox

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   human Synapsin I

   Available since

   Jan. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CaMKIIa-eArchT3.0-P2A-EGFP-WPRE-hGHpA

   Plasmid

   #51110

   Purpose

   Forebrain principal neuron expression of eArchT3,0 with physically uncoupled EGFP fluorophore

   Depositor

   Jonathan Ting

   Insert

   eArchT3.0
   

   Use

   AAV

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKIIa

   Available since

   April 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. XLone-Puro beta-catenin P2A eGFP-NLS

    Plasmid

    #155189

    Purpose

    PiggybacTransposon-based tunable and temporal expression control of mutated beta-catenin and nuclear eGFP

    Depositor

    Xiaoping Bao

    Article

    Chang et al Biomaterials. 2022 May 6;285:121569. doi

    Insert

    beta-catenin (Ctnnb1 Mouse)
    

    Use

    Unspecified; Piggybac transposon

    Tags

    P2A eGFP NLS

    Expression

    Mammalian

    Mutation

    Sites phosphorylated by GSK-3beta have been mutat…

    Promoter

    TRE3G

    Available since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. AttB_ACE2[dEcto]_IRES-mCherry-H2A-P2A-PuroR

    Plasmid

    #171596

    Purpose

    Ectodomain deleted negative control ACE2 from Bxb1 landing pad

    Depositor

    Kenneth Matreyek

    Article

    Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

    Inserts

    ACE2 (ACE2 Human)
    mCherry
    H2A
    Pac
    

    Use

    Synthetic Biology; Promoterless bxb1 dna recombin…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pVEGFR2_TEV(N)_NES_TCS(Q'L)_HA-dCas9(N)_P2A_Puro-WPRE

    Plasmid

    #101104

    Purpose

    Encodes dCas9(N) fused to VEGFR2

    Depositor

    Tudor Fulga

    Article

    Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

    Insert

    VEGFR2-dCas9(N)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV hUbC-Cas9-P2A-Puro_BsmBI-sgRNA-BsmBI

    Plasmid

    #188703

    Purpose

    Lentivirus transfer plasmid encoding Cas9, puromycin resistance, and a BsmBI array for cloning 4 sgRNAs

    Depositor

    David Tobin

    Article

    Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. RabV CVS-N2c(deltaG)-mCherry-P2A-FlpO

    Plasmid

    #73471

    Purpose

    Expresses mCherry and FlpO for monosynaptic circuit mapping and genetic manipulation

    Depositor

    Thomas Jessell

    Article

    Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

    Insert

    mCherry-P2A-FlpO
    

    Use

    Neurotropic virus

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. LZF1735 pAAV_hSyn-DiO-somArchon1-EGFP-P2A-somCheRiff

    Plasmid

    #126512

    Purpose

    Optopatch4. Simultaneous optical recording and manipulation of electrical activity in neurons

    Depositor

    Adam Cohen

    Article

    Fan et al Cell. 2020 Jan 23. pii: S0092-8674(20)30

    Insert

    somArchon1-EGFP-P2A-somCheRiff
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. Human NANOS3-P2A-mCherry knock-in targeting construct

    Plasmid

    #59721

    Purpose

    Gene targeting construct for human NANOS3 locus, introducing a P2A-mCherry reporter gene. Contains an frt-PGK-Neo-PA-frt cassette that can be excised.

    Depositor

    Jacob Hanna

    Article

    Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:

    Insert

    P2A mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-CBE4max-xCas9(3.7)-P2A-EGFP (RTW4637)

    Plasmid

    #140000

    Purpose

    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with xCas9(3.7)(D10A/A262T/R324L/S409I/E480K/E543D/M694I/E1219V) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized CBE4max xCas9(3.7) with P2A-EGFP
    

    Use

    CRISPR

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; xCas9(3.7)=A262T/R324L/S409I/E480K/…

    Promoter

    CAG

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. RabV CVS-N2c(deltaG)-mCherry-P2A-Cre

    Plasmid

    #73472

    Purpose

    Expresses mCherry and Cre for monosynaptic circuit mapping and genetic manipulation

    Depositor

    Thomas Jessell

    Article

    Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

    Insert

    mCherry-P2A-Cre
    

    Use

    Neurotropic virus

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. DIO-EF1a-FLEX-H2B-GFP-P2A-N2c(G)

    Plasmid

    #73476

    Purpose

    Encodes the G protein to enable transsynaptic RAV infection

    Depositor

    Thomas Jessell

    Article

    Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

    Insert

    H2B-GFP-P2A-N2c(G)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDH-EF1S-Nluc-P2A-copGFP-T2A-Puro

    Plasmid

    #73032

    Purpose

    Express fluorescent and luminescence dual reporter

    Depositor

    Kazuhiro Oka

    Insert

    Nluc-P2A-copGFP-T2A-Puro
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Truncated EF1

    Available since

    Jan. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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