We narrowed to 9,536 results for: pho;

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pET28C-mCherry-HNRNPA1LCD

Plasmid

#204405

Purpose

E. coli expression of HNRNPA1 low-complexity domain (186-320) with an N-terminal mCherry tag

Depositor

Samie Jaffrey

Article

Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

Insert

HNRNPA1 (186-320) (HNRNPA1 Human)

Tags

6x-His; mCherry

Expression

Bacterial

Promoter

T7

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pcdna3 flag - ULK2 wt

Plasmid

#27637

Depositor

Reuben Shaw

Article

Egan et al Science. 2011 Jan 28;331(6016):456-61. d

Insert

mouse ULK2 (Ulk2 Mouse)

Tags

flag

Expression

Mammalian

Mutation

Initial M removed from ULK2.

Available Since

March 16, 2011

Availability

Academic Institutions and Nonprofits only
pET21a Wb-iPGM

Plasmid

#180246

Purpose

Bacterial expression plasmid for production of recombinant Wuchereria bancrofti iPGM His10

Depositor

James Inglese

Article

van Neer et al ACS Chem Biol. 2022 Aug 19;17(8):2284-22

Insert

Wuchereria bancrofti iPGM-10His

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pFIN-RK-GFP-mIRBP156-CHER-WPRE

Plasmid

#44351

Depositor

Susan Semple-Rowland

Article

Semple-Rowland et al Mol Vis. 2010 May 27;16:916-34.

Inserts

RK-GFP
mIRBP156-CHER

Use

Lentiviral

Promoter

human rhodopsin kinase (pos 2554-2850) and mouse …

Available Since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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pMSCV mSin1.1 HA

Plasmid

#12582

Depositor

David Sabatini

Article

Frias et al Curr Biol. 2006 Sep 19;16(18):1865-70. d

Insert

mSin1.1 (MAPKAP1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

Nov. 22, 2006

Availability

Academic Institutions and Nonprofits only
MBP-MEK1

Plasmid

#68300

Purpose

N-terminal MBP tagged human MEK1 gene for bacterial expression

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

MBP-MEK1 (MAP2K1 Synthetic, Human)

Tags

His and MBP

Expression

Bacterial

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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MuHKI-pGFPN3

Plasmid

#21919

Depositor

Hossein Ardehali

Article

Sun et al Mol Cell Biol. 2008 Feb . 28(3):1007-17.

Insert

Mutant huamn HKI (with a mutation in the catalytic site in the C-terminal half of the enzyme) in pGFP-N3 (HK1 Human)

Tags

GFP

Expression

Mammalian

Mutation

This is the full length human HKI cDNA sequence w…

Available Since

Nov. 2, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3-3myc-6His-EZH2 21A

Plasmid

#42663

Depositor

Mien-Chie Hung

Article

Cha et al Science. 2005 Oct 14;310(5746):306-10.

Insert

EZH2 (EZH2 Human)

Tags

3Myc and 6His

Expression

Mammalian

Mutation

S21A

Available Since

July 17, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3-3myc-6His-EZH2 21D

Plasmid

#42664

Depositor

Mien-Chie Hung

Article

Cha et al Science. 2005 Oct 14;310(5746):306-10.

Insert

EZH2 (EZH2 Human)

Tags

3Myc and 6His

Expression

Mammalian

Mutation

S21D

Available Since

July 17, 2013

Availability

Academic Institutions and Nonprofits only
pMSCV mSin1.2 myc

Plasmid

#12577

Depositor

David Sabatini

Article

Frias et al Curr Biol. 2006 Sep 19;16(18):1865-70. d

Insert

mSin1.2 (MAPKAP1 Human)

Use

Retroviral

Tags

myc

Expression

Mammalian

Available Since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only
pRK5 Flag Grb10 wt long

Plasmid

#37481

Depositor

David Sabatini

Article

Hsu et al Science. 2011 Jun 10;332(6035):1317-22.

Insert

Grb10 (GRB10 Human)

Tags

Flag

Expression

Mammalian

Mutation

isoform a, 594aa

Available Since

Aug. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pMSCV mSin1.5 myc

Plasmid

#12579

Depositor

David Sabatini

Article

Frias et al Curr Biol. 2006 Sep 19;16(18):1865-70. d

Insert

mSin1.5 (MAPKAP1 Human)

Use

Retroviral

Tags

myc

Expression

Mammalian

Available Since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only
pBABE-puro SHP2 E76A

Plasmid

#8331

Depositor

Ben Neel

Article

Zhang et al Mol Cell Biol 2002 Jun;22(12):4062-72.

Insert

SHP2 (PTPN11 Human)

Use

Retroviral

Expression

Mammalian

Mutation

E76A (activated mutant)

Available Since

June 17, 2005

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.2/V5-DEST SNAP29-V5

Plasmid

#69821

Purpose

Expresses SNAP29-V5 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

Synaptosomal-associated protein 29 (SNAP29 Human)

Use

Retroviral

Tags

V5

Expression

Mammalian

Promoter

cmv

Available Since

April 26, 2016

Availability

Academic Institutions and Nonprofits only

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pMSCV mSin1.4 myc

Plasmid

#12578

Depositor

David Sabatini

Article

Frias et al Curr Biol. 2006 Sep 19;16(18):1865-70. d

Insert

mSin1.4 (MAPKAP1 Human)

Use

Retroviral

Tags

myc

Expression

Mammalian

Available Since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only
pCAG/ATP11C(E184Q)-HA

Plasmid

#209251

Purpose

Mammalian expression of ATP11C mutant E184Q

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

Insert

ATP11C (ATP11C Human)

Tags

HA

Expression

Mammalian

Mutation

E184Q

Available Since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG/ATP8A1(E191Q)-HA

Plasmid

#209240

Purpose

Mammalian expression of ATP8A1 mutant E191Q

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

Insert

ATP8A1 (ATP8A1 Human)

Tags

HA

Expression

Mammalian

Mutation

E191Q

Available Since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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EE-LRRK2

Plasmid

#193583

Purpose

Expresses full-length LRRK2 with FYVE-CC2 domains from HRS and 3xFLAG on the N-terminus

Depositor

Mark Cookson

Article

Kluss et al Proc Natl Acad Sci U S A. 2022 Oct 25;11

Insert

Leucine-rich repeat kinase 2 (LRRK2 Human)

Tags

FYVE-CC2 and 3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCV mSin1.1 myc

Plasmid

#12576

Depositor

David Sabatini

Article

Frias et al Curr Biol. 2006 Sep 19;16(18):1865-70. d

Insert

mSin1.1 (MAPKAP1 Human)

Use

Retroviral

Tags

myc

Expression

Mammalian

Available Since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3-MTS-WT-c-Src-FLAG

Plasmid

#44652

Depositor

Yoshimi Homma

Article

Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.

Insert

v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)

Tags

FLAG tag and mitochondria-targeting sequence (res…

Expression

Mammalian

Promoter

CMV promoter

Available Since

April 26, 2013

Availability

Academic Institutions and Nonprofits only

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pET28aAviHisCD72cCTLD

Plasmid

#129066

Purpose

generation of biotinylated mouse CD72c CTLD protein using bacteria

Depositor

Takeshi Tsubata

Article

Akatsu et al J Exp Med. 2016 Nov 14;213(12):2691-2706

Insert

CD72c (Cd72 Mouse)

Expression

Bacterial

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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TMEM16F-I521K-peGFP-N1

Plasmid

#228588

Purpose

Expresses TMEM16F-I521K-peGFP in mammalian cells

Depositor

Huanghe Yang

Article

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

TMEM16F/anoctamin 6 (Ano6), transcript variant 2 (Ano6 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

I521K plus a 3 amino acid N-terminal truncation (…

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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EGFR-ET1

Plasmid

#44185

Depositor

Timothy Springer

Article

Mi et al Biochemistry. 2008 Sep 30;47(39):10314-2

Insert

EGFR-ET1 (EGFR Human)

Tags

3C cleavge site, protein C peptide tag, his6, SBP…

Expression

Mammalian

Mutation

I104V and D254N relative to NP_005219.2

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pEGFP-C3-PLK4 K41M-3xFLAG

Plasmid

#69838

Purpose

This plasmid encodes kinase dead PLK4 isoform 1 (K41M mutation) with an N-terminal EGFP tag and C-terminal 3xFLAG tag

Depositor

Michel Bornens

Article

Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

Insert

PLK4 (PLK4 Human)

Tags

3xFLAG tag and EGFP

Expression

Mammalian

Mutation

K41M

Promoter

CMV

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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hSUMO1-HIS

Plasmid

#118361

Purpose

This plasmid expresses the full length human SUMO1.

Depositor

Lea Sistonen

Article

Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

Insert

hSUMO1 (SUMO1 Human)

Tags

HIS

Expression

Mammalian

Mutation

Please see Depositor Comments

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCV mSin1.2 HA

Plasmid

#12583

Depositor

David Sabatini

Article

Frias et al Curr Biol. 2006 Sep 19;16(18):1865-70. d

Insert

mSin1.2 (MAPKAP1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

Nov. 30, 2006

Availability

Academic Institutions and Nonprofits only
TMEM16F-I611K-peGFP-N1

Plasmid

#228581

Purpose

Expresses mouse TMEM16F-I611K-eGFP in mammalian cells

Depositor

Huanghe Yang

Article

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

TMEM16F/anoctamin 6 (Ano6), transcript variant 2 (Ano6 Mouse)

Expression

Mammalian

Mutation

I611K plus a 3 amino acid N-terminal truncation (…

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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TrHKI-pGFPN3

Plasmid

#21918

Depositor

Hossein Ardehali

Article

Sun et al Mol Cell Biol. 2008 Feb . 28(3):1007-17.

Insert

Truncated human HKI (lacking exon 1 sequence that encodes for the mitochondrial binding domain) in pGFP-N3 plasmid (HK1 Human)

Tags

GFP

Expression

Mammalian

Mutation

This is the truncated human HKI cDNA (lacking exo…

Available Since

Oct. 1, 2009

Availability

Academic Institutions and Nonprofits only

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pCMV HA hRB delta CDK + T373

Plasmid

#58912

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T373 restored

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with T373 restored

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
sh-SRF

Plasmid

#100797

Purpose

expression of shRNA targeting SRF

Depositor

Joseph Gordon

Article

Mughal et al Cell Death Dis. 2015 Oct 29;6:e1944. doi

Insert

rat SRF

Use

RNAi

Promoter

H1

Available Since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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