We narrowed to 26,705 results for: fer

1. pelB-MBP-BB0323-ss

   Plasmid

   #137067

   Purpose

   E. coli expression clone (T7lac promoter) for mature BB0323 (aa 20-377) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage

   Depositor

   Debra Hansen

   Article

   Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

   Insert

   AAC66700.1
   

   Use

   Tags

   PelB signal peptide + His10 + maltose binding pro…

   Expression

   Bacterial

   Mutation

   mature BB0323: lacks the BB0323 signal peptide

   Promoter

   T7lac

   Available since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pOmnibac_ZZ_linker_ybbR_MAP7

   Plasmid

   #159718

   Purpose

   Expresses MAP7 in Sf9 cells with a ZZ affinity tag and a ybbR tag

   Depositor

   Ahmet Yildiz

   Article

   Ferro et al Science. 2022 Jan 21;375(6578):326-331.

   Insert

   MAP7 (Microtubule Associated Protein 7) (MAP7 Human)
   

   Use

   Tags

   ZZ affinity tag, ybbR tag

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pmiR-report-MYB-WT

   Plasmid

   #141114

   Purpose

   Reporter plasmid of WT MYB in mammalian cells.

   Depositor

   Jianjun Chen

   Article

   Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

   Insert

   CMYB (MYB Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pmiR-report-MYB-mut

   Plasmid

   #141115

   Purpose

   Reporter plasmid of mutant MYB in mammalian cells.

   Depositor

   Jianjun Chen

   Article

   Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

   Insert

   MYB (MYB Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   A to T mutations at m6A sites

   Promoter

   Available since

   Dec. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pPB-EF1A-HALO-hLRRK2 (LIR1 mutant WEVL -> AEVA)

   Plasmid

   #229742

   Purpose

   Expresses Halo tagged human LRRK2 with LIR1 point mutations in mammalian cells. Stable expression with piggybac transposase.

   Depositor

   Shawn Ferguson

   Article

   Bentley-DeSousa et al J Cell Biol. 2025 Feb 3;224(2):e20231015

   Insert

   LRRK2 (LRRK2 Human)
   

   Use

   Tags

   Halo

   Expression

   Mammalian

   Mutation

   LIR1 mutant WEVL -> AEVA

   Promoter

   EF1A

   Available since

   Feb. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pPB-EF1A-HALO-hLRRK2 (LIR2 mutant WTFI -> ATFA)

   Plasmid

   #229743

   Purpose

   Expresses Halo tagged human LRRK2 with LIR2 point mutations in mammalian cells. Stable expression with piggybac transposase.

   Depositor

   Shawn Ferguson

   Article

   Bentley-DeSousa et al J Cell Biol. 2025 Feb 3;224(2):e20231015

   Insert

   LRRK2 (LRRK2 Human)
   

   Use

   Tags

   Halo

   Expression

   Mammalian

   Mutation

   LIR2 mutant WTFI -> ATFA

   Promoter

   EF1A

   Available since

   Feb. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pPB-EF1A-HALO-hLRRK2 (LIR1+2 mutant WEVL -> AEVA / WTFI -> ATFA)

   Plasmid

   #229744

   Purpose

   Expresses Halo tagged human LRRK2 with LIR1 and LIR2 point mutations in mammalian cells. Stable expression with piggybac transposase.

   Depositor

   Shawn Ferguson

   Article

   Bentley-DeSousa et al J Cell Biol. 2025 Feb 3;224(2):e20231015

   Insert

   LRRK2 (LRRK2 Human)
   

   Use

   Tags

   Halo

   Expression

   Mammalian

   Mutation

   LIR1+2 mutant WEVL -> AEVA / WTFI -> ATFA

   Promoter

   EF1A

   Available since

   Feb. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGL3-ACSL4-(-1899)

   Plasmid

   #202514

   Purpose

   Expressing luciferase driven by human ACSL4 promoter (bases -1 to -1899 with respect to exon1 start site)

   Depositor

   Ming Chen

   Article

   Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

   Insert

   ACSL4 promoter (partial) (ACSL4 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGL3-ACSL4-(-706/-277)

   Plasmid

   #202511

   Purpose

   Expressing luciferase driven by human ACSL4 promoter (bases -277 to -706 with respect to exon1 start site)

   Depositor

   Ming Chen

   Article

   Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

   Insert

   ACSL4 promoter (partial) (ACSL4 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGL3-ACSL4-(-254)

    Plasmid

    #202512

    Purpose

    Expressing luciferase driven by human ACSL4 promoter (bases -1 to -254 with respect to exon1 start site)

    Depositor

    Ming Chen

    Article

    Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

    Insert

    ACSL4 promoter (partial) (ACSL4 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGL3-ACSL4-(-706)

    Plasmid

    #202513

    Purpose

    Expressing luciferase driven by human ACSL4 promoter (bases -1 to -706 with respect to exon1 start site)

    Depositor

    Ming Chen

    Article

    Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

    Insert

    ACSL4 promoter (partial) (ACSL4 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pU6-chiRNA_dCrkgRNA1

    Plasmid

    #131139

    Purpose

    Encodes gRNA targeting Drosophila crk locus. Cuts 110bp upstream of the transcription start site. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013).

    Depositor

    Mark Peifer

    Article

    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

    Insert

    dCrk gRNA1 (Crk Fly)
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pU6-chiRNA_dCrkgRNA2

    Plasmid

    #131140

    Purpose

    Encodes gRNA targeting Drosophila crk locus. Cuts 99bp beyond the 3' UTR. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013).

    Depositor

    Mark Peifer

    Article

    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

    Insert

    dCrk gRNA1 (Crk Fly)
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. mini-YFP-hDF-C2F-TM

    Plasmid

    #72600

    Purpose

    mini human dysferlin (from domain F to transmembrane ) tagged with YFP

    Depositor

    Renzhi Han

    Article

    Cheng et al Nat Med. 2014 Oct;20(10):1187-92. doi: 1

    Insert

    dysferlin (DYSF Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    from domain F to transmembrane domain

    Promoter

    CMV

    Available since

    April 15, 2016

    Availability

    Academic Institutions and Nonprofits only

15. CLYBL-TO-hNGN2-BSD-mApple

    Plasmid

    #124229

    Purpose

    Targets the human CLYBL safe harbor locus for integration of dox-inducible NGN2 for differentiation of iPSCs into cortical neurons. Contains a floxed BSD-NLS-mApple selection cassette.

    Depositor

    Michael Ward

    Article

    Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

    Insert

    NGN2 (NEUROG2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pOmnibac_ZZ_linker_K963_GFP_SNAP

    Plasmid

    #159727

    Purpose

    Expresses kinesin-1 (amino acids 1-963) in Sf9 cells with a C-terminal GFP and SNAP tag

    Depositor

    Ahmet Yildiz

    Article

    Ferro et al Science. 2022 Jan 21;375(6578):326-331.

    Insert

    KIF5B (full length, 1-963 amino acids), K963 (KIF5B Human)
    

    Use

    Tags

    GFP and SNAP tags and ZZ affinity tag

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pelB-MBP-BB0238-ss

    Plasmid

    #137046

    Purpose

    E. coli expression clone (T7lac promoter) for mature BB0238 (aa 21-256) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Insert

    AAC66635.2 (BB_0238 Borrelia burgdorferi B31)
    

    Use

    Tags

    PelB signal peptide + His10 + maltose binding pro…

    Expression

    Bacterial

    Mutation

    mature BB0238: lacks the BB0238 signal peptide

    Promoter

    T7lac

    Available since

    April 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pF CAG luc IRES neo

    Plasmid

    #98294

    Purpose

    For constitutive expression of firefly luciferase from the CAG promoter together with an IRES-linked neo resistance gene

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Inserts

    Firefly luciferase
    IRES
    Neomycin resistance gene (NeoR)
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pOmnibac_ZZ_linker_ybbR_Tau

    Plasmid

    #159717

    Purpose

    Expresses tau in Sf9 cells with a ZZ affinity tag and a ybbR tag

    Depositor

    Ahmet Yildiz

    Article

    Ferro et al Science. 2022 Jan 21;375(6578):326-331.

    Insert

    Microtubule-associated protein tau (MAPT Human)
    

    Use

    Tags

    ZZ affinity tag, ybbR tag

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pelB-MBP-P66-ss

    Plasmid

    #137045

    Purpose

    E. coli expression clone (T7lac promoter) for mature P66 (aa 22-618) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Insert

    AAC66949.1 (p66 Borrelia burgdorferi B31)
    

    Use

    Tags

    PelB signal peptide + His10 + maltose binding pro…

    Expression

    Bacterial

    Mutation

    mature P66: lacks the P66 signal peptide

    Promoter

    T7lac

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge