We narrowed to 1,865 results for: lin tian

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pSEM246 - MCS mosTI unc-119 target

Plasmid

#159821

Purpose

Single copy or array insertion by MosTI using split unc-119 selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

MCS mosTI unc-119 target

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pSEM320 - sgRNA mosTI unc-119 array insertion

Plasmid

#159824

Purpose

Single copy or array insertion by MosTI using split unc-119 selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

sgRNA mosTI unc-119 array insertion

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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p415-PtrpC-Cas9-TtrpC-CYC1t

Plasmid

#68059

Purpose

Express humanized Cas9 under the control of trpC promoter and terminator from A. nidulans.

Depositor

Christian Hong

Article

Matsu-Ura et al Fungal Biol Biotechnol. 2015 Jul 15;2:4.

Insert

humanized Cas9

Use

CRISPR; N. crassa, fungi

Expression

Yeast

Available Since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only

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pDestattB_ubi:EGFP (pCM272)

Plasmid

#68339

Purpose

Multisite Gateway destination vector for phiC31-mediated transgenesis in zebrafish, harbors ubiquitin promoter (ubi)-driven EGFP.

Depositor

Christian Mosimann, Leonard Zon

Article

Mosimann et al Dev Dyn. 2013 Aug;242(8):949-63. doi: 10

Insert

EGFP

Use

Zebrafish transgenesis

Promoter

ubi

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pSEM376 - sgRNA - spc4 - array - insertion - unc-119 - locus

Plasmid

#182344

Purpose

sgRNA4 for insertion of extrachromosomal arrays into the ce-unc-119 genomic locus. Use with pSEM371 fragment included in Ex array.

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

Spacer 4

Expression

Worm

Available Since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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pCK082 desmaMCS:minprommCherry,exorh:mCerulean

Plasmid

#200015

Purpose

Expression construct with desmin a regulatory elements, mouse beta-globin minimal promoter mCherry, exorh:mCerulean

Depositor

Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Type

Empty backbone

Use

Zebrafish expression

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pSEM272 - MCS mosTI Pmlc-2_GFP target

Plasmid

#159834

Purpose

Single copy or array insertion by MosTI using split Pmlc-2 GFP selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

MCS mosTI Pmlc-2_GFP target

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pU6-DHFR

Plasmid

#80329

Purpose

Allows for expression of an sgRNA in Toxoplasma gondii and confers resistance to pyrimethamine

Depositor

Sebastian Lourido

Article

Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

Insert

DHFR-TSc3

Use

CRISPR

Promoter

DHFR-TS 5' UTR

Available Since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pSEM255 - sgRNA mosTI Pmlc-2_GFP

Plasmid

#159835

Purpose

Single copy or array insertion by MosTI using split Pmlc-2 GFP selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

sgRNA mosTI Pmlc-2_GFP

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pSEM396 - mosTI unc-119 - NNN - operon - gfp - tbb-2

Plasmid

#182353

Purpose

GFP co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::gfp::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Expression

Worm

Available Since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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pU6-SAG1-DHFR

Plasmid

#80322

Purpose

Encodes a CRISPR sgRNA that allows for mutation of the surface antigen SAG1 in Toxoplasma gondii and confers resistance to pyrimethamine

Depositor

Sebastian Lourido

Article

Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

Insert

SAG1 sgRNA

Use

CRISPR

Promoter

U6

Available Since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-tagRFP-NLS-HA-Bxb1

Plasmid

#65625

Purpose

Vector for cotranslational expression of tagRFP-Bxb1

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

Bxb1 integrase

Tags

HA, SV40 NLS, and tagRFP

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

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pSEM388 - mosTI unc-119 - NNN - mScarlet - tbb-2

Plasmid

#182348

Purpose

mScarlet fusion cloning vector for mosTI(unc-119). Transgene inserted upstream of mScarlet and tbb-2 3' UTR. MCS or Golden Gate (BsaI: tgcc - MCS - aaaa)

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Expression

Worm

Available Since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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pKB33 (pUC19-KANMX-tetO7-CYCpr)

Plasmid

#210472

Purpose

Plasmid with KanMX-tetO7-CYCpr cassette from the yTHC collection. Used for amplifying cassette with homology to regions of interest for genomic integration.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Insert

KanMX-TetO7-CYCpr cassette

Use

Synthetic Biology

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCK081 desmaMCS:minpromCerulean,exorh:mCherry

Plasmid

#200014

Purpose

Expression construct with desmin a regulatory elements, mouse beta-globin minimal promoter mCerulean, exorh:mCherry

Depositor

Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Type

Empty backbone

Use

Zebrafish expression

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pU6-Decoy

Plasmid

#80324

Purpose

Encodes Cas9 and a CRISPR sgRNA that alleviates toxicity to Cas9 in Toxoplasma gondii

Depositor

Sebastian Lourido

Article

Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

Insert

Decoy sgRNA

Use

CRISPR

Promoter

U6

Available Since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

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attB-GFP-Poly(A)

Plasmid

#65524

Purpose

Plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

attB-GFP

Use

Mouse Targeting; Bxb1

Expression

Mammalian

Available Since

Aug. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pSEM390 - mosTI unc-119 - Peft-3 - NNN - tbb-2

Plasmid

#182350

Purpose

Ubiquitous expression cloning vector for mosTI(unc-119). Transgene inserted between Peft-3 and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Expression

Worm

Available Since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

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pDN0606 (pDEST-DHFR F[3] N-term,HygR)

Plasmid

#210488

Purpose

pDEST vector to tag gene of interest with DHFR F[3] on the N-terminus to perform DHFR-PCA in S. cerevisiae.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Type

Empty backbone

Use

Synthetic Biology; Destination vector for gateway…

Expression

Yeast

Promoter

ADH1

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pKB11 (pDEST-DHFR F[1,2] C-term, NatR)

Plasmid

#210485

Purpose

pDEST vector to tag gene of interest with DHFR F[1,2] on the C-terminus to perform DHFR-PCA in S. cerevisiae.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Type

Empty backbone

Use

Synthetic Biology; Destination vector for gateway…

Expression

Yeast

Promoter

ADH1

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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