Addgene

We narrowed to 372 results for: abo.5

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pMSCV-FKBP-IRSp53(trunc C5)

Plasmid

#221277

Purpose

Expression of IRSp53 C-terminal truncation 5 (247-275) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

IRSp53 C-terminal truncation 5 (247-275) (Baiap2 Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Expression

Mutation

Promoter

Available since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pROS_phleo-Tps1/Gsy2

Plasmid

#196612

Purpose

Encoding guide RNAs for the knock out of TPS1 and GSY2 genes

Depositor

Matthias Heinemann

Article

Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

Insert

TPS1, GSY2 (TPS1, GSY2 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

SNR52

Available since

March 23, 2023

Availability

Academic Institutions and Nonprofits only

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pUGP1.1

Plasmid

#196615

Purpose

Plasmid used for the C-terminal tagging of Ugp1 with mCherry and the auxin-inducible degron in yeast

Depositor

Matthias Heinemann

Article

Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

Insert

UGP1::mCherry-AID(71-114)-NatMX (UGP1 Budding Yeast)

Use

Tags

AID(71-114) and mCherry

Expression

Yeast

Mutation

Promoter

Native promoter

Available since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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pIRES2-EGFP-deltaNp53:WTp53

Plasmid

#49244

Purpose

expresses human p53 deltaN linked to full length p53 with C terminal His tag and also expresses EGFP in mammalian cells

Depositor

Dylan Taatjes

Article

Lin et al Aging Cell. 2013 Oct;12(5):863-72. doi:

Insert

p53 deltaN and p53 WT (TP53 Human)

Use

Tags

His and IRES-EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pDUET-aaCPRct-A13AMO

Plasmid

#20116

Depositor

Jay Keasling

Article

Chang et al Nat Chem Biol. 2007 May . 3(5):274-7.

Insert

p450 reductase and Amorphadiene oxidase

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
pET-28b C7H1G6_6H N-terminal

Plasmid

#223434

Purpose

C7H1G6 GNAT expression vector with N-terminal His6 tag

Depositor

Emily Balskus

Article

Mehta et al Nat Med. 2023 Mar;29(3):700-709. doi: 10

Insert

C7H1G6 GNAT

Use

Tags

His6

Expression

Mutation

Promoter

Available since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pET-28b R6CZ24_6H C-terminal

Plasmid

#223435

Purpose

R6CZ24 Thiolase expression vector with C-terminal His6 tag

Depositor

Emily Balskus

Article

Mehta et al Nat Med. 2023 Mar;29(3):700-709. doi: 10

Insert

R6CZ24 Thiolase

Use

Tags

His6

Expression

Mutation

Promoter

Available since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pET-28b Salmonella NAT_6H C-terminal

Plasmid

#223436

Purpose

Salmonella NAT expression vector with C-terminal His6 tag

Depositor

Emily Balskus

Article

Mehta et al Nat Med. 2023 Mar;29(3):700-709. doi: 10

Insert

Salmonella NAT

Use

Tags

His6

Expression

Mutation

Promoter

Available since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pSLQ4428 pHR (EF1a-mCherry-P2A-RfxCas13d-2xNLS-ecDHFR-3xFLAG-WPRE)

Plasmid

#214885

Purpose

Lentiviral vector encoding TMP-regulatable RfxCas13d-DD fusion

Depositor

Stanley Qi

Article

Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

Insert

EF1a-mCherry-P2A-RfxCas13d-2xNLS-ecDHFR-3xFLAG-WPRE

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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pQCXIB Flag MFF WT

Plasmid

#74384

Purpose

Retroviral expression vector containing Flag tagged WT MFF isoform 5

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

Flag-MFF (MFF Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2016

Availability

Academic Institutions and Nonprofits only
pIRESh-GFPII-SM-Dlk1

Plasmid

#108932

Purpose

GFP-tagged vector expressing full length isoform of Dlk1

Depositor

Anne Ferguson-Smith

Article

Ferron et al Nature. 2011 Jul 20;475(7356):381-5. doi

Insert

Dlk1 (Dlk1 Mouse)

Use

Tags

GFPII

Expression

Mammalian

Mutation

Promoter

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti.PGK.blast-Flag-MFF-WT

Plasmid

#74379

Purpose

Lentiviral expression vector containing flag tagged WT MFF isoform 5

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

Flag-MFF (MFF Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

PGK

Available since

April 13, 2016

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag MFF iso5 S172A

Plasmid

#74382

Purpose

Flag tagged human S172A MFF isoform 5 mutant

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

Flag-MFF (MFF Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Ser 172 Ala (numbering based on MFF isoform 1)

Promoter

Available since

April 13, 2016

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag MFF iso5 S155,172A

Plasmid

#74383

Purpose

Flag tagged human S155,172A MFF isoform 5 mutant

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

Flag-MFF (MFF Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

See 155 Ala, Ser 172 Ala (numbering based on MFF …

Promoter

Available since

April 13, 2016

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag MFF iso5 S155A

Plasmid

#74381

Purpose

Flag tagged human S155A MFF isoform 5 mutant

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

Flag-MFF (MFF Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Ser 155 Ala (numbering based on MFF isoform 1)

Promoter

Available since

April 13, 2016

Availability

Academic Institutions and Nonprofits only
mCherry-CRY2-INPP5E

Plasmid

#79561

Purpose

expression of mCherry-tagged CRY2PHR fused to inositol 5-phosphatase domain of human INPP5E with mutated C-terminal CaaX-motif

Depositor

Olof Idevall-Hagren, Pietro De Camilli

Article

Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

Insert

INPP5E (INPP5E Human)

Use

Tags

CRY2 (photolyase homology region domain of Arabid…

Expression

Mammalian

Mutation

C-terminal region, aa 214_644, C641A mutation to …

Promoter

CMV

Available since

July 11, 2016

Availability

Academic Institutions and Nonprofits only
pLenti.PGK.blast-Flag-MFF-S155,172D

Plasmid

#74442

Purpose

Lentiviral expression vector containing flag tagged S155,172D MFF isoform 5 mutant

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

Flag-MFF (MFF Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Ser 155 Asp, Ser 172 Asp (Numbering based on MFF …

Promoter

Available since

May 10, 2016

Availability

Academic Institutions and Nonprofits only
pLenti.PGK.blast-Flag-MFF-S155,172A

Plasmid

#74380

Purpose

Lentiviral expression vector containing flag tagged S155,172A MFF isoform 5 mutant

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

Flag-MFF (MFF Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Ser 155 Ala, Ser 172 Ala (Numbering based on MFF …

Promoter

Available since

April 14, 2016

Availability

Academic Institutions and Nonprofits only
pLenti.PGK.blast-Flag-MFF-S155,172E

Plasmid

#74443

Purpose

Lentiviral expression vector containing flag tagged S155,172E MFF isoform 5 mutant

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

Flag-MFF (MFF Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Ser 155 Glu, Ser 172 Glu (Numbering based on MFF …

Promoter

Available since

April 14, 2016

Availability

Academic Institutions and Nonprofits only
pQCXIB Flag MFF S155,172A

Plasmid

#74387

Purpose

Retroviral expression vector containing Flag tagged S155,172A MFF isoform 5 mutant

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

Flag-MFF (MFF Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Ser 155 Ala, Ser 172 Ala (Numbering based on MFF …

Promoter

CMV

Available since

May 13, 2016

Availability

Academic Institutions and Nonprofits only