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pEM9177

Plasmid

#213994

Purpose

PrpsM driven expression of SSAGSAAGS-mGFPmut2

Depositor

Marc Erhardt

Article

Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

Insert

SSAGSAAGS-mGFPmut2

Tags

SSAGSAAGS

Expression

Bacterial

Promoter

PrpsM

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pEM10688

Plasmid

#213997

Purpose

PrpsM driven expression of mNeptune25

Depositor

Marc Erhardt

Article

Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

Insert

mNeptune2.5

Expression

Bacterial

Promoter

PrpsM

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pEM8734

Plasmid

#213993

Purpose

PrpsM driven expression of Ypet

Depositor

Marc Erhardt

Article

Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

Insert

Ypet

Expression

Bacterial

Promoter

PrpsM

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pEM12395

Plasmid

#213999

Purpose

PrpsM driven expression of mVenusNB

Depositor

Marc Erhardt

Article

Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

Insert

mVenusNB

Expression

Bacterial

Promoter

PrpsM

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pEM10689

Plasmid

#213998

Purpose

PrpsM driven expression of miniSOG

Depositor

Marc Erhardt

Article

Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

Insert

miniSOG

Expression

Bacterial

Promoter

PrpsM

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pEM13825

Plasmid

#214000

Purpose

PrpsM driven expression of eGFP

Depositor

Marc Erhardt

Article

Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

Insert

eGFP

Expression

Bacterial

Promoter

PrpsM

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pEM8318

Plasmid

#213990

Purpose

PrpsM driven expression of sfmTurquoise2ox

Depositor

Marc Erhardt

Article

Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

Insert

sfmTurquoise2ox

Expression

Bacterial

Promoter

PrpsM

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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BB3_arscen_URA3_Hygro

Plasmid

#118077

Purpose

low copy plasmid, neg. control

Depositor

Michael Sauer

Article

Steiger et al Metab Eng. 2019 Mar;52:224-231. doi: 10.

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDS3-X4

Plasmid

#70140

Purpose

Yeast chr. integration vector with DS3 selection marker

Depositor

Morten Sommer

Article

Rugbjerg et al Metab Eng. 2015 Sep;31:189-97. doi: 10.1

Insert

DS3

Expression

Yeast

Promoter

AgTEF1

Available Since

Dec. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pYD1

Plasmid

#73447

Purpose

expression vector designed for expression, secretion, and display of proteins on the extracellular surface of Saccharomyces cerevisiae cells

Depositor

Dane Wittrup

Article

Kieke et al Protein Eng. 1997 Nov;10(11):1303-10.

Type

Empty backbone

Tags

6xHis, AGA2, T7 peptide, V5 tag, and Xpress tag

Expression

Yeast

Promoter

GAL1

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pTS395_PhCMV-SB100

Plasmid

#169633

Purpose

Expression vector encoding PhCMV driven SB100 sleeping beauty transposase (PhCMV-SB100-pA)

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven SB100

Expression

Mammalian

Promoter

PhCMV

Available Since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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pSMART-HCKan-EV

Plasmid

#202466

Purpose

Empty vector of pSMART-HCKan

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pTrcECT

Plasmid

#75477

Purpose

Inducible expression of ectABC in E. coli

Depositor

Xixian Xie

Article

Ning et al Metab Eng. 2016 Mar 9;36:10-18. doi: 10.

Insert

ectABC

Expression

Bacterial

Promoter

trc

Available Since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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pSLQ9663 - ACTB GFP-P2A CLIP Donor

Plasmid

#202688

Purpose

CLIP donor template for integrating GFP into ACTB locus. Encodes GFP with ACTB homology arms.

Depositor

Stanley Qi

Article

Chavez et al Nat Biomed Eng. 2023 May 1. doi: 10.1038

Insert

CopGFP

Available Since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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pVEC1

Plasmid

#241910

Purpose

Primary screening of PET hydrolases and enzyme condition screening. TphR-based terephthalate (TPA) biosensor

Depositor

Neil Dixon

Article

Alvarez Gonzalez et al Metab Eng Commun. 2024 Nov 6;19:e00250.

Insert

tphR, tphK, sfGFP

Use

Synthetic Biology

Mutation

none

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pCTCON2-Aga1p

Plasmid

#158141

Purpose

Expresses Aga1p in yeast

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

Aga1p

Expression

Yeast

Promoter

GAL10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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p274 EF1a_EGFP_hH11

Plasmid

#164851

Purpose

EGFP-expression vector for human H11 locus

Depositor

Philip Jordan

Article

Pryzhkova et al Tissue Eng Regen Med. 2020 Apr;17(2):223

Insert

EGFP

Expression

Mammalian

Promoter

EF1-alpha

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pEEK2

Plasmid

#83492

Purpose

Expression plasmid for conjugational transfer into cyanobacteria

Depositor

Pia Lindberg

Article

Miao et al Metab Eng Commun. 2017 Jul 29;5:45-53. d

Type

Empty backbone

Use

Synthetic Biology

Tags

Strep-tag

Expression

Bacterial

Promoter

Ptrc core

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pBEST-OR2-OR1-Pr-UTR1-deGFP-T500

Plasmid

#40019

Depositor

Vincent Noireaux

Article

Shin et al J Biol Eng. 2010 Jun 24;4:8.

Insert

deGFP

Expression

Bacterial

Promoter

OR2-OR1-Pr (bacteriophage Lambda with one mutatio…

Available Since

Oct. 1, 2012

Availability

Academic Institutions and Nonprofits only

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pENTR-p14FAST

Plasmid

#50025

Purpose

pENTR vector for reptilian orthoreovirus p14 fusion-associated small transmembrane (FAST) protein

Depositor

Jamie Davies

Article

Cachat et al J Biol Eng. 2014 Nov 19;8(1):26. eCollec

Insert

p14FAST

Use

Entry vector

Available Since

Dec. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pVH21-Tier1-PhEF1a

Plasmid

#169561

Purpose

Tier-1 PhEF1a-driven expression vector (PhEF1a-MCS-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Type

Empty backbone

Expression

Mammalian

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pBA2kIKmA2

Plasmid

#39213

Depositor

Anastasios Melis

Article

Lindberg et al Metab Eng. 2010 Jan;12(1):70-9. Epub 200

Insert

Kudzu Isoprene Synthase

Use

Synthetic Biology

Mutation

Deleted the predicted chloroplast transit peptide

Promoter

psbA2

Available Since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only
pIYC08

Plasmid

#64743

Purpose

Yeast expression plasmid: acsL641P, ALD6, ADH2, ERG10

Depositor

Jens Nielsen

Article

Chen et al Metab Eng. 2013 Jan;15:48-54. doi: 10.10

Insert

ADH2, ALD6, acsL641P, ERG10

Expression

Yeast

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only

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pTS1019_Tier3(PB)

Plasmid

#169649

Purpose

Tier-3 vector for stable integration of up to three cassettes via piggyBac transposase (ITR-A1::A2::A3-ITR)

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Type

Empty backbone

Expression

Mammalian

Available Since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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p273 EF1a_mCherry2_hH11

Plasmid

#164852

Purpose

mCherry2-expression vector for human H11 locus

Depositor

Philip Jordan

Article

Pryzhkova et al Tissue Eng Regen Med. 2020 Apr;17(2):223

Insert

mCherry2

Expression

Mammalian

Promoter

EF1-alpha

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pMST-713

Plasmid

#118081

Purpose

ptet-on::cexA::ttrpC

Depositor

Michael Sauer

Article

Steiger et al Metab Eng. 2019 Mar;52:224-231. doi: 10.

Insert

CexA

Promoter

ptet-on

Available Since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pSpal2At

Plasmid

#78286

Purpose

Expresses A. thaliana PAL2 in E. coli

Depositor

David Nielsen

Article

McKenna et al Metab Eng. 2011 Sep;13(5):544-54. doi: 1

Insert

PAL2 (PAL2 Mustard Weed)

Expression

Bacterial

Mutation

*see below

Promoter

lac

Available Since

June 1, 2016

Availability

Academic Institutions and Nonprofits only

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pEM7066

Plasmid

#214004

Purpose

PrpsM driven expression of ssrA-tagged mScarlet-I (LVA)

Depositor

Marc Erhardt

Article

Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

Insert

mScarlet-I-LVA

Expression

Bacterial

Promoter

PrpsM

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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cp196V1.2

Plasmid

#52100

Purpose

A improved cpmCherry variant

Depositor

Robert Campbell

Article

Carlson et al Protein Eng Des Sel. 2013 Dec;26(12):763

Insert

cp196V1.2

Tags

6 His tag

Expression

Bacterial

Promoter

pBAD promotor

Available Since

April 14, 2014

Availability

Academic Institutions and Nonprofits only
JBEI-12050

Plasmid

#84982

Purpose

Isopentenol production

Depositor

Taek Soon Lee

Article

Kang et al Metab Eng. 2016 Mar;34:25-35. doi: 10.10

Insert

MevTo-T21-MK-aphA

Expression

Bacterial

Promoter

LacUV5

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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