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Plasmid#158718PurposeCRISPR-assisted-NHEJ system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses NHEJ machinery of M. marinum and recX.DepositorInsertrecX
UseTagsExpressionBacterialMutationPromoterPmycAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR E2 HSPter-35Sp-mito
Plasmid#158727PurposePlant mitoTALEN vector assembly, Entry clone 2 with attL1 L4DepositorInsertA terminator of Arabidopsis heat shock protein, CAMV35S promoter, and mitochondrial presequence
UseOtherTagsExpressionMutationPromoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-DPP4-G418
Plasmid#158453PurposeConstitutive lentiviral expression of human DPP4.DepositorInsertDPP4 (DPP4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR E3 pR6A L3-L2
Plasmid#158733PurposePlant mitoTALEN vector assembly, Entry clone 3 with attL3 L2 (last NG)DepositorInsertTALEN ORF without TAL repeat
UseTALENTagsExpressionMutationPromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR E3 pR7A L3-L2
Plasmid#158734PurposePlant mitoTALEN vector assembly, Entry clone 3 with attL3 L2 (last NI)DepositorInsertTALEN ORF without TAL repeat
UseTALENTagsExpressionMutationPromoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR E1 pF7A L1-L4
Plasmid#158730PurposePlant mitoTALEN vector assembly, Entry clone 1 with attR4 R3 (last NI)DepositorInsertTALEN ORF without TAL repeat
UseTALENTagsExpressionMutationPromoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR E1 pF6A L1-L4
Plasmid#158729PurposePlant mitoTALEN vector assembly, Entry clone 1 with attR4 R3 (last NG)DepositorInsertTALEN ORF without TAL repeat
UseTALENTagsExpressionMutationPromoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
mTAGBFP2 Peroxisome visual barcode
Plasmid#158668Purposetagging clonal populations with Peroxisomal mTAGBFP2 fluorescent protein. Adding Peroxisome localization signal to mTAGBFP2 WCDepositorInsertmTAGBFP2
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG::Nucbow
Plasmid#158996PurposeMulticolor nuclear-restricted labeling with a genome-integrating piggyBac transposon vector (randomly expressing H2B-tethered mEYFP, mCherry or mCerulean upon Cre recombination)DepositorInsertNucbow
UseTagsAll FPs are fused to H2BExpressionMammalianMutationPromoterCAGAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ132-tRNA2.0
Plasmid#158394PurposeGolden Gate entry vector to express the 2nd gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterWithout promoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pXPR_502_sgCD45
Plasmid#158705PurposeCD45 CRISPRa controlDepositorInsertCD45 (PTPRC Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR SFFV GLP1R(S301A)-EGFP
Plasmid#158123PurposeLentiviral plasmid for expression of GLP1R(S301)-EGFP fusion with SFFV promoter.DepositorInsertGLP1R (GLP1R )
UseLentiviralTagsEGFPExpressionMammalianMutationSerine 301 changed to alanine to enhance membrane…PromoterSFFVAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pdCas9- AsiA_m2.1
Plasmid#158065PurposeExpressing evolved CasTA of dCas9 fused AsiA variant 2.1DepositorInsertdCas9-AsiA_m2.1
UseTagsExpressionBacterialMutationPromoterTetOAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDOC-K-glmS-GFPfor
Plasmid#158059PurposeDonor plasmid for insertion of eGFP and the constitutive acpP promoter into the S. Typhimurium chromosome. GFP is in the forward orientation relative to the kanamycin resistance selectable markerDepositorInsertGreen Fluorescent Protein optimised for excitation with UV light
UseSynthetic BiologyTagsExpressionBacterialMutationPromoteracpPAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLIK 3xFLAG-MAVS hygro
Plasmid#158634PurposeLentiviral expression vector for an inducible 3xFLAG-tagged MAVS constructDepositorInsertMAVS (MAVS Human)
UseLentiviralTags3XFLAGExpressionMutationPromoterAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
GAL FLAG25QDProGFP p306
Plasmid#15831DepositorInserthtt 25Q Delta Pro (HTT Human)
UseTagsFLAG and GFPExpressionYeastMutationProline-rich region removedPromoterAvailable sinceOct. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-SomaGCaMP7
Plasmid#158759PurposeCell body-targeted GCaMP7f, under synapsin promoter: GCaMP7f followed by a linker, and the EE-RR coiled coil motif (GCaMP7f-27-EE-RR). As bright as conventional GCaMP7f.DepositorInsertSomaGCaMP7
UseAAVTagsExpressionMammalianMutationPromotersynapsin promoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
PLX306 iCre tetO tomato rtTA
Plasmid#158050Purposelenti-viral construct with Cre recombinase and doxycycline inducible expression of tdTomatoDepositorInserttdTomato
UseTagsnoneExpressionMammalianMutationPromotertetO promoterAvailable sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pscALPSpuro-HsACE2 (human)
Plasmid#158081PurposeExpresses human ACE2 in mammalian cellsDepositorInsertHuman ACE2 (ACE2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-HA_mouse_Adar2_Tv1
Plasmid#158110Purposeexpresses mADAR2 shortest transcriptDepositorInsertmAdar2 (Adarb1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only