We narrowed to 17,417 results for: URE

1. pSaPIPLC His

   Plasmid

   #173810

   Purpose

   Expression and purification of mature, His-tagged Staphylococcus aureus (strain FPR3757) phosphaatidyl inositol specific phospholipase C (PI-PLC) from E. coli BL21 CodonPlus (DE3) RIL cells

   Depositor

   Mary F Roberts, Anne Gershenson

   Article

   Goldstein et al Biochemistry. 2012 Mar 27;51(12):2579-87

   Insert

   Staphylococcus aureus plc
   

   Use

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   none

   Promoter

   T7

   Available since

   Aug. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA

   Plasmid

   #61591

   Purpose

   A single vector AAV-Cas9 system containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA.

   Depositor

   Feng Zhang

   Article

   Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

   Inserts

   hSaCas9
   Chimeric guide for SaCas9
   

   Use

   AAV and CRISPR

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX602-AAV-TBG::NLS-SaCas9-NLS-HA-OLLAS-bGHpA;U6::BsaI-sgRNA

   Plasmid

   #61593

   Purpose

   A single vector AAV-Cas9 system containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA.

   Depositor

   Feng Zhang

   Article

   Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

   Inserts

   hSaCas9
   Chimeric guide for SaCas9
   

   Use

   AAV and CRISPR

   Tags

   HA, NLS, and OLLAS tag

   Expression

   Mammalian

   Mutation

   K175R and K736R (deUb mutations)

   Promoter

   Available since

   Feb. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. SaMscL (CDelta26)

   Plasmid

   #79027

   Purpose

   Staphylococcus aureus MscL with C Terminal Truncation of 26 amino acids

   Depositor

   Douglas Rees

   Article

   Liu et al Nature. 2009 Sep 3;461(7260):120-4. doi:

   Insert

   MscL
   

   Use

   Tags

   6x Histidine Tag

   Expression

   Mutation

   Stop codon placed before 26 amino acids at C Term…

   Promoter

   T7 Promoter

   Available since

   June 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-CaMKIIa-C1C2-EYFP

   Plasmid

   #35519

   Depositor

   Karl Deisseroth

   Article

   Kato et al Nature. 2012 Jan 22. doi: 10.1038/nature

   Insert

   ChR1-ChR2 Chimera
   

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKIIa

   Available since

   June 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-CaMKIIa-C1C2-TS-EYFP

   Plasmid

   #35520

   Depositor

   Karl Deisseroth

   Article

   Kato et al Nature. 2012 Jan 22. doi: 10.1038/nature

   Insert

   ChR1-ChR2 Chimera
   

   Use

   Lentiviral

   Tags

   Trafficking signal and EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKIIa

   Available since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAVi U6_gRNA CMV_SadCas9-KRAB

   Plasmid

   #214609

   Purpose

   All-in-one AAVi plasmid expressing S. aureus dCas9-KRAB with sgRNA cassette

   Depositor

   Ralf Gilsbach

   Article

   Laurette et al Circ Res. 2023 Dec 22. doi: 10.1161/CIRC

   Inserts

   gRNA
   SadCas9
   

   Use

   AAV

   Tags

   NP NLS and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A, N580A

   Promoter

   CMV and U6

   Available since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. p3A2

   Plasmid

   #234793

   Purpose

   Mammalian expression of bovine arrestin-2

   Depositor

   Vsevolod Gurevich

   Article

   Pan et al J Biol Chem. 2003 Mar 28;278(13):11623-3

   Insert

   Arrestin-2 (ARRB2 Bovine)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. p3A2-RE

   Plasmid

   #234794

   Purpose

   Mammalian expression of bovine arrestin-2 (R169E)

   Depositor

   Vsevolod Gurevich

   Article

   Pan et al J Biol Chem. 2003 Mar 28;278(13):11623-3

   Insert

   Arrestin-2 (ARRB2 Bovine)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R169E

   Promoter

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA

    Plasmid

    #61592

    Purpose

    A human codon-optimized Cas9 from Staphylococcus aureus (SaCas9).

    Depositor

    Feng Zhang

    Article

    Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

    Has Service

    AAV6

    Insert

    hSaCas9
    

    Use

    AAV and CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. BPK2101

    Plasmid

    #65770

    Purpose

    Bacterial expression plasmid for S. aureus Cas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSaCas9-NLS-3xFLAG-T7-BsaIcassette-Sa-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized SaCas9, and SaCas9 gRNA
    

    Use

    CRISPR

    Tags

    NLS-3xFlag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 (x2)

    Available since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYL112-19

    Plasmid

    #15459

    Depositor

    Chia Lee

    Article

    Luong et al J Microbiol Methods. 2007 Jul . 70(1):18

    Insert

    S. aureus phage L54a integrase
    

    Use

    S.aureus-e. coli shuttle vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 9, 2007

    Availability

    Academic Institutions and Nonprofits only

13. pSR1037

    Plasmid

    #208736

    Purpose

    Characterization plasmid for PSarA1 (sfgfp expressed from PSarA1 on the S. aureus pC194 replicon)

    Depositor

    Lauren Andrews

    Article

    Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

    Insert

    PSarA1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSR1038

    Plasmid

    #208737

    Purpose

    Characterization plasmid for PCap (sfgfp expressed from PCap on the S. aureus pC194 replicon)

    Depositor

    Lauren Andrews

    Article

    Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

    Insert

    PCap
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PCap

    Available since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-AsCas12f1-v5.1+T1-empty

    Plasmid

    #204977

    Purpose

    AsCas12f1-v5.1+T1-based Human cell genome editing

    Depositor

    Quanjiang Ji

    Article

    Wu et al Nature Catalysis (2023) 6, 695–709

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    N70Q/K103R/A104R/S118A/D364R

    Promoter

    PCMV,U6

    Available since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. LacD_WT_pET21a

    Plasmid

    #29785

    Depositor

    SeWon Suh

    Article

    Lee et al FEBS Lett. 2011 Jan 21. 585(2):307-12.

    Insert

    Tagatose 1,6-diphosphate aldolase
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 7, 2011

    Availability

    Academic Institutions and Nonprofits only

17. LacD_L165E_pET21a

    Plasmid

    #29786

    Depositor

    SeWon Suh

    Article

    Lee et al FEBS Lett. 2011 Jan 21. 585(2):307-12.

    Insert

    Tagatose 1,6-diphosphate aldolase
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    L165E

    Promoter

    Available since

    July 7, 2011

    Availability

    Academic Institutions and Nonprofits only

18. LacD_L275S_pET21a

    Plasmid

    #29787

    Depositor

    SeWon Suh

    Article

    Lee et al FEBS Lett. 2011 Jan 21. 585(2):307-12.

    Insert

    Tagatose 1,6-diphosphate aldolase
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    L275S

    Promoter

    Available since

    July 7, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pTcI39-switch

    Plasmid

    #195557

    Purpose

    Temperature state switch expressing low-temperature mWasabi and high-temperature mRFP1

    Depositor

    Mikhail Shapiro

    Article

    Xiong et al ACS Synth Biol. 2022 Jul 15;11(7):2518-2

    Inserts

    mRFP1
    mWasabi
    TcI39
    

    Use

    Tags

    6x His and AANDENYADAS degradation tag

    Expression

    Bacterial

    Mutation

    A67T

    Promoter

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCasSA

    Plasmid

    #98211

    Purpose

    CRISPR-based E. coli/Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus using S. pyogenes Cas9

    Depositor

    Quanjiang Ji

    Article

    Chen et al J Am Chem Soc. 2017 Mar 15;139(10):3790-

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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