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Plasmid#74511PurposeOverexpression of Grap2 in mammalian cellsDepositorInsertGrap2 (GRAP2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA CrkII Y221F
Plasmid#50732Purposemammalian expression of CrkII Y221F mutantDepositorInsertCrkII Y221F (CRK Human)
UseTagsExpressionMammalianMutationY221FPromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAB043c
Plasmid#79223PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ lambda cI-p38-alpha; PlacIq lacI
UseTagsExpressionBacterialMutationPromoterPlacZ; PlacIqAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGex-4T-1 Jip4 (1-272)
Plasmid#59002PurposeJIP4 serves as an activator of the p38 mitogen-activated protein (MAP) kinase pathway by a mechanism that requires the MAP kinase kinases MKK3 and MKK6DepositorInsertMapk8ip4 (Spag9 Mouse)
UseTagsGstExpressionBacterialMutationJip4 (aa1-272)PromoterAvailable sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 T7 Jip4 (1-272)
Plasmid#58928PurposeJIP4 serves as an activator of the p38 mitogen-activated protein (MAP) kinase pathway by a mechanism that requires the MAP kinase kinases MKK3 and MKK6DepositorInsertMapk8ip4 (Spag9 Mouse)
UseTagsT7ExpressionMammalianMutationJip4 (1-272aa)PromoterAvailable sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 T7 Jip4 (1-151)
Plasmid#58926PurposeJIP4 serves as an activator of the p38 mitogen-activated protein (MAP) kinase pathway by a mechanism that requires the MAP kinase kinases MKK3 and MKK6DepositorInsertMapk8ip4 (Spag9 Mouse)
UseTagsT7ExpressionMammalianMutationJip4 (1-151aa)PromoterAvailable sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 T7 Jip4 (1-212)
Plasmid#58927PurposeJIP4 serves as an activator of the p38 mitogen-activated protein (MAP) kinase pathway by a mechanism that requires the MAP kinase kinases MKK3 and MKK6DepositorInsertMapk8ip4 (Spag9 Mouse)
UseTagsT7ExpressionMammalianMutationJip4 (1-212aa)PromoterAvailable sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCALNL-tdTomato-2A-mSyp::EGFP
Plasmid#191213PurposeExpresses tdTomato protein and mouse Synaptophysin protein fused with EGFP in Cre expressing mammalian cells.DepositorInserttdTomato-T2A-mSyp::EGFP (Syp Mouse)
UsePcagenTagsmSyp::EGFPExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcFLAG-Grap2-R83E
Plasmid#74512PurposeOverexpression of Grap2 R83E in mammalian cellsDepositorInsertGrap2 (GRAP2 Human)
UseTagsFLAGExpressionMammalianMutationR83EPromoterCMVAvailable sinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
CMV-synaptophysin-Lime
Plasmid#201016PurposeExpresses Lime in synaptic vesicle lumen by fusion to synaptophysinDepositorInsertSynaptophysin-Lime (Syp Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR-ERKKTRClover
Plasmid#59138PurposeORF encoding ERK KTR mClover flanked by Gateway sequencesDepositorInsertERK Kinase Translocation Reporter (MAPK1 Human, Mouse)
UseTagsExpressionMutationPromoterAvailable sinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Synaptophysin-TdTomato-IRES2-OHT-Cre
Plasmid#134308PurposeExpresses Synaptophysin-TdTomato and also tamoxifen dependant Cre (OHT-Cre, aka Cre-ER)DepositorInsertsUseTagsTdTomatoExpressionMammalianMutationPromoterCAGAvailable sinceJuly 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
3xFlag-tdMCP-Erk2 (pErk reporter)
Plasmid#226167PurposeUsed to transiently express the pErk reporter in LABEL-seq MAPK activity assays.DepositorInsert3xFlag-tdMCP-Erk2 (MAPK1 Human)
UseTags3xFlag-tdMCPExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB-hSyn-mSyp-RT1-24xGCN4
Plasmid#106300PurposeThe plasmid encodes mouse Synaptophysin sparsely labeled with SunTag24x_v4.DepositorInsertmSyp-RT1-24xGCN4 (Syp Mouse)
UseTags24xGCN4ExpressionMammalianMutationPromoterhuman Synapsin1Available sinceMarch 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETM33_CRK_SH3
Plasmid#178472PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertCRK_SH3 (CRK Synthetic, Human)
UseTagsHis-GSTExpressionBacterialMutationPromoterT7/LacOAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T3-HA-ERK2 GOF
Plasmid#53200PurposeExpresses GST and HA tagged human ERK2 R67S D321N from bacterial expression vectorDepositorInsertERK2 (MAPK1 Human)
UseTagsGST and HAExpressionBacterialMutationR67S/D321NPromoterAvailable sinceJune 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSAD-5PSD95-EGFP-SynPhRFP
Plasmid#217979PurposeG-Deleted Rabies genomic plasmid to express 5PSD95-EGFP and SynPhRFPDepositorUseG-deleted rabiesTagsEGFP and RFPExpressionMutationPromoterNoneAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_MAPK14-P2A-Hygro_Barcode
Plasmid#170239PurposeBarcoded lentiviral vector to express MAPK14 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorInsertMAPK14 (MAPK14 Human)
UseLentiviralTagsV5ExpressionMutationPromoterEF1aAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T3-HA-ERK2 R67S
Plasmid#53201PurposeExpresses GST and HA tagged human ERK2 R67S from bacterial expression vectorDepositorInsertERK2 (MAPK1 Human)
UseTagsGST and HAExpressionBacterialMutationR67SPromoterAvailable sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFIN-RK-GFP-WPRE
Plasmid#44358DepositorInsertRK-GFP (MAPK14 Synthetic, Human)
UseLentiviralTagsExpressionMutationPromoterhuman rhodopsin kinase (pos 2557-2853)Available sinceMay 8, 2013AvailabilityAcademic Institutions and Nonprofits only