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Showing: 161 - 180 of 59511 results

  1. lentiCas9-Blast (Lentiviral Prep)

    Viral prep
    #52962-LV
    Purpose
    Ready-to-use Lentiviral Prep particles produced from lentiCas9-Blast (#52962). In addition to the viral particles, you will also receive purified lentiCas9-Blast plasmid DNA. Lentiviral particles carrying Cas9 and blasticidin resistance. This virus is used to make stable cell lines expressing Cas9.
    Depositor
    Feng Zhang
    Publication
    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:
    Promoter
    EFS-NS
    Available since
    July 14, 2016
    Availability
    Academic Institutions and Nonprofits only

  2. lentiCRISPR v2-Blast

    Plasmid
    #83480
    Purpose
    Mammalian expression of Cas9 and sgRNA scaffold
    Depositor
    Mohan Babu
    Insert
    Blasticidin S deaminase
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    Replaced puromycin N-acetyltransferase on the ori…
    Promoter
    EF-1α
    Available since
    Nov. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    83480-plasmid-map-sequence-id-423158

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  3. PX552

    Plasmid
    #60958
    Purpose
    pAAV-U6sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). AAV plasmid for sgRNA cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei.
    Depositor
    Feng Zhang
    Publication
    Swiech et al Nat Biotechnol. 2014 Oct 19. doi: 10.103
    Inserts
    U6_(SpaI)_sgRNA
    Syn_EGFP-KASH
    Use
    AAV, CRISPR, and Mouse Targeting
    Expression
    Mammalian
    Promoter
    Synapsin and U6
    Available since
    Nov. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    60958-plasmid-map-sequence-id-382829

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  4. pAAV-hSyn-DIO-hM3D(Gq)-mCherry (AAV2)

    Viral prep
    #44361-AAV2
    Purpose
    Ready-to-use AAV2 particles produced from pAAV-hSyn-DIO-hM3D(Gq)-mCherry (#44361). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM3D(Gq)-mCherry plasmid DNA. Syn-driven, Cre-dependent, hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Publication
    Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d
    Promoter
    Syn
    Tags
    mCherry (Cre-dependent)
    Available since
    Nov. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  5. pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA

    Plasmid
    #20297
    Purpose
    Cre-activated AAV expression of humanized ChR2 with H134R mutation fused to mCherry for optogenetic activation
    Depositor
    Karl Deisseroth
    Has service
    AAV1, AAV5, AAV8, AAV9, and AAV Retrograde
    Insert
    channelrhodopsin-2
    Use
    AAV
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    humanized ChR2 gene with histidine 134 changed to…
    Promoter
    EF1alpha
    Available since
    June 23, 2009
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    20297-plasmid-map-sequence-id-354478

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  6. FHRE-Luc

    Plasmid
    #1789
    Purpose
    Reporter plasmid for FOXO3a. Forkhead responsive element with luciferase readout.
    Depositor
    Michael Greenberg
    Publication
    Brunet et al Cell 1999 Mar 19;96(6):857-68.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FHRE (FOXO3 Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    3 copies of FRE
    Available since
    Jan. 5, 2006
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1789-plasmid-map-sequence-id-339925

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  9. pcDNA3-TLR9-YFP

    Plasmid
    #13642
    Depositor
    Doug Golenbock
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Toll-Like Receptor 9 (TLR9 Human)
    Tags
    YFP
    Expression
    Mammalian
    Available since
    June 12, 2007
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    13642-plasmid-map-sequence-id-392328

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  10. pAAV-hSyn-hChR2(H134R)-EYFP

    Plasmid
    #26973
    Purpose
    AAV expression of humanized ChR2 with H134R mutation fused to EYFP driven by human Synapsin I promoter for optogenetic excitation
    Depositor
    Karl Deisseroth
    Has service
    AAV1, AAV2, AAV5, AAV9, AAV PHP.eB, and AAV Retrograde
    Insert
    hChR2(H134R)
    Use
    AAV
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    H134R
    Promoter
    hSyn
    Available since
    Feb. 4, 2011
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    26973-plasmid-map-sequence-id-199313

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  11. pMT-puro

    Plasmid
    #17923
    Purpose
    Insect expression vector
    Depositor
    David Sabatini
    Type
    Empty backbone
    Use
    Drosophila metallothionein gene promoter drives e…
    Expression
    Insect
    Available since
    April 29, 2008
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    17923-plasmid-map-sequence-id-211593

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  12. pAAV-hSyn-hM4D(Gi)-mCherry (AAV8)

    Viral prep
    #50475-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-hSyn-hM4D(Gi)-mCherry (#50475). In addition to the viral particles, you will also receive purified pAAV-hSyn-hM4D(Gi)-mCherry plasmid DNA. hSyn-driven hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    Syn
    Tags
    mCherry
    Available since
    Oct. 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  13. Lamp1-RFP

    Plasmid
    #1817
    Purpose
    Mammalian expression of Lamp1 fused to RFP. Used for localization to the lysosome.
    Depositor
    Walther Mothes
    Publication
    Sherer et al Traffic 2003 Nov;4(11):785-801.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    lysosome associated membrane protein 1 (Lamp1 Rat)
    Tags
    RFP
    Expression
    Mammalian
    Mutation
    D50E (not important for function of plasmid)
    Available since
    Sept. 26, 2005
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1817-plasmid-map-sequence-id-350820

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  15. pAAV-Ef1a-mCherry-IRES-Cre (AAV Retrograde)

    Viral prep
    #55632-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-Ef1a-mCherry-IRES-Cre (#55632). In addition to the viral particles, you will also receive purified pAAV-Ef1a-mCherry-IRES-Cre plasmid DNA. Cre and bicistronic (IRES) mCherry expression under the EF1a promoter. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Publication
    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:
    Promoter
    EF1a and Ef1a/IRES
    Tags
    mCherry
    Available since
    Aug. 30, 2017
    Availability
    Academic Institutions and Nonprofits only

  16. Human sgRNA library Brunello in lentiCRISPRv2

    Pooled library
    #73179
    Purpose
    Human sgRNA library in backbone lentiCRISPRv2 targeting 19,114 genes and containing 76,441 unique sgRNAs along with 1000 non-targeting controls
    Depositor
    John Doench, David Root
    Publication
    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103
    Has service
    Lentiviral Prep
    Expression
    Mammalian
    Use
    CRISPR and Lentiviral
    Available since
    Feb. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  17. pAAV2/7

    Plasmid
    #112863
    Purpose
    AAV packaging plasmid expressing Rep/Cap genes
    Depositor
    James M. Wilson
    Insert
    Rep2/Cap7
    Use
    AAV
    Promoter
    Truncated P5
    Available since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    112863-plasmid-map-sequence-id-364707

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  18. pFGC-pcoCas9

    Plasmid
    #52256
    Purpose
    A binary plasmid containing 35SPPDK:pcoCas9:NOS cassette for plant expression of Cas9 and multiple cloning sites for insertion of guide RNA
    Depositor
    Jen Sheen
    Insert
    35SPPDK:pcoCas9:NOS cassette
    Use
    CRISPR; Plant expression
    Promoter
    constitutive 35SPPDK promoter
    Available since
    March 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    52256-plasmid-map-sequence-id-116013

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  19. pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato] (AAV5)

    Viral prep
    #62723-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato] (#62723). In addition to the viral particles, you will also receive purified pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato] plasmid DNA. Cre-dependent ChrimsonR-tdTomato under the control of the Synapsin promoter. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Publication
    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:
    Promoter
    Syn
    Tags
    tdTomato (Cre-dependent)
    Available since
    July 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  20. pAAV.CMV.PI.EGFP.WPRE.bGH

    Plasmid
    #105530
    Purpose
    AAV expression of EGFP from CMV promoter
    Depositor
    James M. Wilson
    Has service
    AAV1, AAV2, AAV5, AAV8, AAV9, AAV PHP.eB, AAV Retrograde, and AAV rh10
    Insert
    EGFP
    Use
    AAV
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    105530-plasmid-map-sequence-id-454420

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Showing: 161 - 180 of 59511 results