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Plasmid#170902PurposeGoldenBraid Double Transcriptional Unit - pG10-90_E-Luc_AtACT2t+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…PromoterpG10-90Available sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2 3'UTR GAC
Plasmid#110401PurposeLuminescence-based glutaminase GAC isoform 3'UTR-mediated RNA stability reporterDepositorInsertGLS glutaminase (GLS Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterSV40Available sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IZ-SEC61B-mCherry-FRB
Plasmid#233349PurposeStable expression of SEC61B-mCherry-FRB in mammalian cells. FRB localizes in the ER lumen.DepositorInsertsUseRetroviralTagsExpressionMammalianMutationT2098L mutant of FKBP-rapamycin binding domain of…PromoterAvailable sinceApril 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSAM_AraC
Plasmid#91569PurposeMariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Arabinose promoter expresses transposase. Ampicillin resistance on backbone.DepositorInsertAraC/PBAD
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-TAPBPR
Plasmid#135500PurposeMammalian expression of FLAG-tagged TAPBPRDepositorInsertTAPBPR (TAPBPL Human)
UseTagsLuminal FLAG epitope tag and Signal peptide from …ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV EFIa DIO LMO11 (GeNL_SS-ChRmine)
Plasmid#219426PurposeCre-dependent expression of luciferase opsin fusion protein for bioluminescent optogeneticsDepositorInsertmNeonGreen, ISN-IE, ChRmine
UseAAVTagsGaussia luciferase signal sequenceExpressionMutationPromoterEF-1alphaAvailable sinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
SacI ML GFP Strand 11 Long
Plasmid#164120PurposeDetect the long Mitochondria-Endoplasmic reticulum contact along with the OMMGFP1-10DepositorInsertSacI ML GFP Strand 11 Long
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRK172-6xHis-0N4R-P301S-tau-HiBiT
Plasmid#187835PurposeExpression of 6xHis-tagged P301S tau of 0N4R isoform with a C-terminal HiBiT tag for bimolecular luminescence with LgBiT.DepositorInsert6XHis-0N4R-P301S-tau-HiBiT
UseTags6xHistidine and HiBiTExpressionBacterialMutationPromoterT7Available sinceSept. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/er-(n2)oxStayGold(c4)
Plasmid#185822PurposeA soluble photostable fluorescent marker of the ER lumen. The StayGold gene has no codon optimization for mammalian expression.DepositorInserter-(n2)oxStayGold(c4)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
SacI ML GFP Strand 11 Short
Plasmid#164121PurposeDetect the short Mitochondria-Endoplasmic reticulum contact along with the OMMGFP1-10DepositorInsertSacI ML GFP Strand 11 Short
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
LiPOP1-NanoLuc
Plasmid#168265PurposeChemo-optogenetic control of necroptotic cell death by coupling LiPOP1 with NanoLuc-aided bioluminescence optogenetic stimulation (NanoLOGS)DepositorInsertMLKL(1-125) (MLKL Human)
UseTagsmCherry, NanoLuc, CRY2-PHRExpressionMammalianMutationH15A, K16A, R17APromoterCMVAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCherry-CRY2-5ptaseOCRL(D523G)
Plasmid#66837PurposeExpression of inositol 5-phosphatase domain of OCRL(D523G - catalytically inactive mutant). Binds to CIBN upon blue light illumination.DepositorInsert5ptaseOCRL (OCRL Human)
UseTagsmCherry-CRY2ExpressionMammalianMutationD523GPromoterCMVAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
TfR-mCh-DHFR
Plasmid#173013PurposeFor light regulated release of transferrin receptor (TfR) from the endoplasmic reticulum. Driven by CMV promoter.DepositorInsertTfR-mCh-DHFR
UseTagsDHFR and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO-VMAT2-WPRE-pA
Plasmid#39339DepositorInsertSlc18a2 (Slc18a2 Mouse)
UseAAV; RTagsExpressionMutationMyc tag inserted in the first lumenal loop betwee…PromoterAvailable sinceOct. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pPHR-iRFP713
Plasmid#103818PurposeSoluble BLInCR mock effector that is recruited to 'localizer' sites upon blue light illuminationDepositorInsertPHR-iRFP713 (CRY2 Mustard Weed)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
DHFR-GFP-th-NL1
Plasmid#173007PurposeFor light regulated release of neuroligin 1 from the endoplasmic reticulum. Driven by synapsin promoter. Thrombin site is included between GFP and NL1.DepositorInsertDHFR-GFP-NL1
UseTagsDHFR and EGFPExpressionMammalianMutationPromoterhuman synapsinAvailable sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CAG-LMO1 (GLuc-ChR2-EYFP)
Plasmid#72888Purposefusion protein of Gaussia luciferase, Channelrhodopsin-2, and enhanced yellow fluorescent protein for bioluminescent optogeneticsDepositorInsertGaussia luciferase, Channelrhodopsin-2, EYFP
UseTagsExpressionBacterial and MammalianMutationPromoterCAGAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
DHFR-GFP-th-GluA1
Plasmid#173008PurposeFor light regulated release of GluA1 from the endoplasmic reticulum. Driven by synapsin promoter. Thrombin site is included between GFP and GluA1.DepositorInsertDHFR-GFP-th-GluA1
UseLentiviralTagsGFPExpressionMammalianMutationPromoterhuman synapsinAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP EGFP-ER [TM(SAC1)]
Plasmid#186475PurposeExpression of the Endoplasmic Reticulum marker GFP-ER [TM(SAC1)] in mammalian cellsDepositorInsertSAC1 (SACM1L Human)
UseRetroviralTagsEGFPExpressionMammalianMutationC-terminal transmembrane region of the protein: 5…PromoterAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-EYFP
Plasmid#103815PurposeSoluble BLInCR construct that can interact with PHR constructs upon illumination with blue lightDepositorInsertCIBN (CIB1 Mustard Weed)
UseTagsEYFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBBK51 pHomL-RPL18Bp-Neon(gfp)-Link-SCS2tmh-SSA1t-HomR (AmpR)
Plasmid#179035PurposeVector containing a yeast integration cassette for RPL18B-driven expression of Neon(gfp)-Link-SCS2tmh, a marker for the endoplasmic reticulumDepositorInsertRPL18Bp-Neon(gfp)-Link-SCS2tmh
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCIneoLuc-PP1A
Plasmid#128348PurposeVector for LUMIER assayDepositorInsertPP1A (PPP1CA Human)
UseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-mCherry
Plasmid#103816PurposeSoluble BLInCR construct that can interact with PHR constructs upon illumination with blue lightDepositorInsertCIBN (CIB1 Mustard Weed)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPHR-mCherry-NLS
Plasmid#103819PurposeSoluble BLInCR mock effector that is recruited to 'localizer' sites upon blue light illuminationDepositorInsertPHR-mCherry-NLS (CRY2 Mustard Weed)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPHR-EYFP-NLS
Plasmid#103820PurposeSoluble BLInCR mock effector that is recruited to 'localizer' sites upon blue light illuminationDepositorInsertPHR-EYFP-NLS (CRY2 Mustard Weed)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBBK92 pHomL-TDH3p-Scarlet(rfp)-Link-SCS2tmh-SSA1t-HomR (AmpR)
Plasmid#179076PurposeVector containing a yeast integration cassette for expressing TDH3p-Scarlet(rfp)-Link-SCS2tmh, an overexpressed (bright) marker for the endoplasmic reticulumDepositorInsertTDH3p-Scarlet(rfp)-Link-SCS2tmh
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK72 pHomL-RPL18Bp-Scarlet(rfp)-Link-SCS2tmh-SSA1t-HomR (AmpR)
Plasmid#179056PurposeVector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-SCS2tmh, a marker for the endoplasmic reticulumDepositorInsertRPL18Bp-Scarlet(rfp)-Link-SCS2tmh
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPHR-EYFP
Plasmid#103817PurposeSoluble BLInCR mock effector that is recruited to 'localizer' sites upon blue light illuminationDepositorInsertPHR-EYFP (CRY2 Mustard Weed)
UseTagsEYFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCerulean3-ER-5
Plasmid#55418PurposeLocalization: Endoplasmic Reticulum, Excitation: 433, Emission: 475DepositorInsertER (CALR Human)
UseTagsmCerulean3ExpressionMammalianMutationER Signal PeptidePromoterCMVAvailable sinceDec. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN_NES_V5_eMagAF_C74_Sec61b
Plasmid#235637PurposeEncodes ALIBi C-terminal fragment, residues 74-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, nuclear export sequence, Sec61b for targeting ALIBi to endoplasmic reticulumDepositorInsertALIBi C-terminal fragment
UseTagsNES-V5-eMagAF and Sec61bExpressionMammalianMutationresidues 74-321PromoterAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMBS-872
Plasmid#218015PurposeNanoLuc luminescence reporterDepositorInsertNanoLuc
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBCC086
Plasmid#202213PurposeLevel 2 empty backbone for strain-specific barcode DNA tag through Tn7 bacterial insertion, Gentamicin selectable marker and far-red-FP fluorescent marker mPlumDepositorInsertpBCC25, pBCC33, pBCC55, pBCC27, pBCC26, pBCC28, pICH41822 (G5)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBCC091
Plasmid#202218PurposeLevel 2 empty backbone for strain-specific barcode DNA tag through Tn7 bacterial insertion, Tetracyline selectable marker and far-red-FP fluorescent marker mPlumDepositorInsertpBCC25, pBCC30, pBCC55, pBCC27, pBCC26, pBCC28, pICH41822 (G5)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBCC100
Plasmid#202226PurposeLevel 2 empty backbone for strain-specific barcode DNA tag through Tn7 bacterial insertion, Kanamycin selectable marker and far-red-FP fluorescent marker mPlumDepositorInsertpBCC25, pBCC32, pBCC55, pBCC27, pBCC26, pBCC28, pICH41822 (G5)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
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PDE4B-Sensor
Plasmid#208929Purposein vitro JNK-Sensor for luminescent detection of JNK-activityDepositorInsertPDE4B docking sequence+Phospho site and Smbit (PDE4B Human)
UseTagsHISx6, MBP, and SmbitExpressionBacterialMutationPromoterT7Available sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBBK91 pHomL-TDH3p-Neon(gfp)-Link-SCS2tmh-SSA1t-HomR (AmpR)
Plasmid#179075PurposeVector containing a yeast integration cassette for expressing TDH3p-Neon(gfp)-Link-SCS2tmh, an overexpressed (bright) marker for the endoplasmic reticulumDepositorInsertTDH3p-Neon(gfp)-Link-SCS2tmh
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV Tmem43-V5
Plasmid#175111PurposeLentiviral expression of mouse Tmem43-V5DepositorInsertTmem43 (Tmem43 Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2 3'UTR KGA
Plasmid#110402PurposeLuminescence-based glutaminase KGA isoform 3'UTR-mediated RNA stability reporterDepositorInsertGLS glutaminase (GLS Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterSV40Available sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CMV-LMO2 (GLuc-VChR1-EYFP)
Plasmid#72889Purposefusion protein of Gaussia luciferase, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogeneticsDepositorInsertsGaussia luciferase, Volvox channelrhodopsin 1, EYFP
Volvox channelrhodopsin 1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pET30a-HS-Tn5
Plasmid#127916PurposeProkaryotic expression of Hyperstable Tn5 (HS-Tn5) for Illumina library preperation. Developed for the large scale ChIP-Seq and ATAC-Seq experiments for the fruitENCODE and Maize Cistrome projects.DepositorInsertHyperstable Tn5
UseTagsE coli elongation factor Ts and Mxe intein - Chit…ExpressionBacterialMutationE54K, L372PPromoterT7Available sinceJuly 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-luciferase
Plasmid#55764Purposeluciferase reporter for in vivo bioluminescence imagingDepositorInsertCMV enhancer/chicken beta actin promoter
UseTagsExpressionMammalianMutationPromoterCMV enhancer/chicken beta actin promoterAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEFIRES-P-ACSL3-mCherry
Plasmid#87158PurposeFluorescent protein targeted to lipid droplets (LDs) from the endoplasmic reticulum (ER)DepositorInsertACSL3 (ACSL3 Human)
UseTagsmCherryExpressionMammalianMutationPromoterEF-1-alphaAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSEP-LAMP1-RFP
Plasmid#167970PurposeExpresses the chimeric construct SEP-LAMP1-RFP where superecliptic pHluorin (SEP) is fused to the luminal domain of LAMP1-RFPDepositorInsertLAMP1 (Lamp1 Rat)
UseTagsSEP and mRFPExpressionMammalianMutationD50EPromoterCMVAvailable sinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-Calr-KDEL-IRES-Puromycin
Plasmid#134664PurposeExpresses EGFP-tagged endoplasmic reticulum markerDepositorInsertcalreticulin (CALR Human)
UseLentiviralTagsEGFPExpressionMammalianMutationdeleted amino acids 18-413PromoterEF1aAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
DHFR-mNeon-GluA1
Plasmid#173009PurposeFor light regulated release of GluA1 from the endoplasmic reticulum. Driven by synapsin promoter. Intracellular trafficking can be better visualized with mNeon.DepositorInsertDHFR-mNeon-GluA1
UseTagsDHFR and mNeonExpressionMammalianMutationPromoterChicken beta actin (CAG)Available sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
ER-CanlonicSF
Plasmid#178343PurposeTo explore endoplasmic reticulum lactate dynamicsDepositorInsertER-CanlonicSF
UseTagsER Retention Signal, ER Signal Exon 1, and ER Sig…ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-Cybt5-IRES-Puromycin
Plasmid#134869PurposeExpresses EGFP-tagged endoplasmic reticulum markerDepositorInsertcytochrome b5 type A (CYB5A Human)
UseLentiviralTagsEGFPExpressionMammalianMutationdeleted amino acids 1-98PromoterEF1aAvailable sinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-NLS-CBGreen-FIRE-puro
Plasmid#139195PurposeLuminescent reporter of ERK activityDepositorInsertCBG99
UseLuciferase and RetroviralTagsFra1 PEST domain fragment (FIRE) and nuclear loca…ExpressionMammalianMutationSee depositor comments belowPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only