We narrowed to 5,339 results for: 290

1. pLenti-UMI

   Plasmid

   #222694

   Purpose

   CRISPR-screening

   Depositor

   Ulrich Elling

   Article

   Michlits et al Nat Methods. 2017 Dec;14(12):1191-1197.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   PGK without BsmBI cutsite

   Promoter

   Available Since

   Aug. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFsynW SYT1 reporter

   Plasmid

   #197275

   Purpose

   Encodes for viral expression of wild type Synaptotagmin 1 (SYT1) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).

   Depositor

   Edwin Chapman

   Article

   Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

   Insert

   SYT1 and HaloTag (Syt1 Rat)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   N/A

   Promoter

   hSyn (human synapsin I promoter)

   Available Since

   July 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. PIK3CA-WT (pFastBac)

   Plasmid

   #16644

   Depositor

   Bert Vogelstein

   Article

   Samuels et al Science. 2004 Apr 23. 304(5670):554.

   Insert

   PIK3CA (PIK3CA Human)
   

   Use

   Baculoviral

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 10, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCALNL-NaChBac

   Plasmid

   #125580

   Purpose

   Cre-dependent expression of NaChBac (FLAG-tagged) in mammalian cells.

   Depositor

   Takeshi Imai

   Article

   Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

   Insert

   NaChBac
   

   Use

   Cre/Lox

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1 LIC 6A CARD8-FLAG

   Plasmid

   #169948

   Purpose

   Mammalian expression of CARD8-FLAG

   Depositor

   Hao Wu

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBEL2319

   Plasmid

   #195697

   Purpose

   Cryo-EM of 6xHis-Foldon-(Gly-Ser-Linker)-MlaC

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

   Insert

   Foldon-MlaC
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   6xHis-Foldon-(Gly-Ser-Linker)-MlaC

   Promoter

   Available Since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pETDuet RagA-RagC

   Plasmid

   #99641

   Purpose

   Bacterial expression of wildtype Rag GTPase dimer

   Depositor

   Kuang Shen, David Sabatini

   Article

   Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

   Inserts

   RagA (RRAGA Human)
   RagC (RRAGC Human)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Dec. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCR1319

   Plasmid

   #111116

   Purpose

   Expresses UBE2T_+_202311076.23-P1P2 sgRNA in pCR1068

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

   Insert

   UBE2T gRNA (UBE2T Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDB.His.MBP.3C CARD8 CARD WT

   Plasmid

   #164022

   Purpose

   Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 UPA-CARD

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Tags

   His6-MBP-3C

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMM2-hPMCA4b

    Plasmid

    #47759

    Purpose

    mammalian expression of human PMCA4b

    Depositor

    Emanuel Strehler, John Penniston

    Article

    Adamo et al Biochem J. 1992 Aug 1;285 ( Pt 3):791-7.

    Insert

    PMCA4b (ATP2B4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available Since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST

    Plasmid

    #105448

    Purpose

    Cation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites in a viral vector

    Depositor

    Hillel Adesnik

    Article

    Pegard et al Nat Commun. 2017 Oct 31;8(1):1228. doi:

    Has Service

    AAV9

    Insert

    ChrimsonR-mRuby2-ST
    

    Use

    AAV

    Tags

    mRuby2 and soma targeting motif

    Expression

    Mutation

    K176R in Chrimson

    Promoter

    hSyn

    Available Since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-mCherry-CyrCR1

    Plasmid

    #128768

    Purpose

    Expresses mCherry in mammalian cells. ~200 nt of Cyrano inserted in 3'UTR with miR-7 site replaced by miR-17 site.

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    Cyrano conserved region (CR1) with intact miR-7 site (OIP5-AS1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. PET-28a-YTH domain of YTHDF3

    Plasmid

    #228777

    Purpose

    Protein expression plasmid for YTH domain of YTHDF3 in E.Coli.

    Depositor

    Huiqing Zhou

    Article

    Wang et al RSC Chem Biol. 2024 Jul 25;5(9):914-923.

    Insert

    YTH domain of YTHDF3 (YTHDF3 Human)
    

    Use

    Tags

    6*His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. hHSF1 K80R

    Plasmid

    #118350

    Purpose

    Plasmid expresses hHSF1 with K80 in DBD mutated to R.

    Depositor

    Lea Sistonen

    Article

    Westerheide et al Science. 2009 Feb 20. 323(5917):1063-6.

    Insert

    hHSF1 (HSF1 Human)
    

    Use

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    K80R

    Promoter

    Available Since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG/ATP10D(E215Q)-HA

    Plasmid

    #209248

    Purpose

    Mammalian expression of ATP10D mutant E215Q

    Depositor

    Hye-Won Shin

    Article

    Naito et al J Biol Chem. 2015 Jun 12;290(24):15004-1

    Insert

    ATP10D (ATP10D Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    E215Q

    Promoter

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.2/Capsid

    Plasmid

    #120816

    Purpose

    Ectopic expression of ZIKV protein

    Depositor

    Jan Rehwinkel

    Article

    Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

    Insert

    Capsid ZIKV
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.2/Envelope

    Plasmid

    #120821

    Purpose

    Ectopic expression of ZIKV protein

    Depositor

    Jan Rehwinkel

    Article

    Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

    Insert

    Envelope ZIKV
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pU6-sgRNA EF1Alpha-puro-T2A-BFP.CYRANO

    Plasmid

    #128755

    Purpose

    Expresses CYRANO gRNA for CRISPRi

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    CYRANO sgRNA (OIP5-AS1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available Since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBH-UAS-secA5-YFP

    Plasmid

    #32359

    Purpose

    Live imaging of apoptotic cells in zebrafish

    Depositor

    Randall Peterson

    Article

    van Ham et al FASEB J. 2010 Nov;24(11):4336-42. Epub 2

    Insert

    secreted human annexin 5 (ANXA5 Human)
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    UAS

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMXs-Ngn2

    Plasmid

    #32928

    Purpose

    Retroviral expression of mouse Ngn2

    Depositor

    Kevin Eggan

    Article

    Son et al Cell Stem Cell. 2011 Sep 2;9(3):205-18.

    Insert

    Ngn2 (Neurog2 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    5'LTR

    Available Since

    Nov. 30, 2011

    Availability

    Academic Institutions and Nonprofits only