We narrowed to 2,376 results for: 683

1. pcDNA3.1-SLC25A46[R257A,R347A]-P2A-Electra1 (pAH36)

   Plasmid

   #227620

   Purpose

   Overexpression of SLC25A46 with R247A and R347A mutations in mammalian cell lines

   Depositor

   Luke Chao

   Article

   Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

   Insert

   SLC25A46 (SLC25A46 Human)
   

   Use

   Tags

   P2A-mElectra1

   Expression

   Mammalian

   Mutation

   R257A,R347A

   Promoter

   CMV, T7

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. GPR6-DuET

   Plasmid

   #213286

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   GPR6 (GPR6 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA 3.1 myc-Rab10-mito

   Plasmid

   #208367

   Purpose

   Mammalian expression of myc-tagged human Rab10 with C-terminal mito signal

   Depositor

   Suzanne Pfeffer

   Article

   Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

   Insert

   myc-Rab10-mito (RAB10 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   wildtype

   Promoter

   Available since

   Dec. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET15b+His SUMO Rab10(Q68L)

   Plasmid

   #207342

   Purpose

   Bacterial expression of His SUMO Rab10(Q68L)

   Depositor

   Suzanne Pfeffer

   Article

   Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

   Insert

   Rab10 (RAB10 Human)
   

   Use

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   Q68L

   Promoter

   Available since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAc5.1B-lambdaN-HA-DmAGO2-RC_B

   Plasmid

   #145981

   Purpose

   Insect Expression of DmAGO2-RC

   Depositor

   Elisa Izaurralde

   Article

   Rehwinkel et al Mol Cell Biol. 2006 Apr . 26(8):2965-75.

   Insert

   DmAGO2-RC (AGO2 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   A deletion of AA 51-56 and an insertion of 23 AA …

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. H2A-mTurquoise-CDC42-G12V-deltaCAAX

   Plasmid

   #176094

   Purpose

   Constitutively active, nuclear localized Cdc42

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

   Insert

   CDC42 (CDC42 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   G12V

   Promoter

   Available since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Opto-GPR6

   Plasmid

   #106002

   Purpose

   Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR6; Opto-GPR6; A4)

   Depositor

   Harald Janovjak

   Article

   Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

   Insert

   Opto-GPR6 (GPR6 Bovine, Human)
   

   Use

   Tags

   Rhodopsin-1D4 and VSV-G

   Expression

   Mammalian

   Mutation

   Chimeric receptor protein

   Promoter

   CMV

   Available since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGL3-Basic-Fascin-Promoter (-210-0)

   Plasmid

   #89826

   Purpose

   Fascin promoter for luciferase assay

   Depositor

   Shengyu Yang

   Article

   Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

   Insert

   Fascin1 (FSCN1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   SV40

   Available since

   May 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. FPGT-TNNI3K gRNA (BRDN0001145543)

   Plasmid

   #75902

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human FPGT-TNNI3K

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   FPGT-TNNI3K,TNNI3K (FPGT-TNNI3K Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. FPGT-TNNI3K gRNA (BRDN0001147862)

    Plasmid

    #75903

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human FPGT-TNNI3K

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    FPGT-TNNI3K,TNNI3K (FPGT-TNNI3K Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. FPGT-TNNI3K gRNA (BRDN0001147836)

    Plasmid

    #75904

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human FPGT-TNNI3K

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    FPGT-TNNI3K,TNNI3K (FPGT-TNNI3K Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. FPGT-TNNI3K gRNA (BRDN0001148036)

    Plasmid

    #75905

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human FPGT-TNNI3K

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    FPGT-TNNI3K,TNNI3K (FPGT-TNNI3K Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLentipuro3/TO/V5-GW/EGFP-Firefly Luciferase

    Plasmid

    #119816

    Purpose

    3rd generation lentiviral plasmid for expression of EGFP Firefly Luciferase fusion protein in mammalian cells

    Depositor

    Ethan Abel

    Article

    Lorenzatti Hiles et al Nat Protoc. 2019 Mar;14(3):738-755. doi:

    Inserts

    Luciferase
    Puromycin
    

    Use

    Lentiviral and Luciferase

    Tags

    EGFP and V5

    Expression

    Mammalian

    Mutation

    Stop codon removed in the frame of the V5 frame v…

    Promoter

    CMV/TO and Human Phosphoglycerate kinase

    Available since

    Jan. 2, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. GFP-RAB10

    Plasmid

    #130883

    Purpose

    Expresses human RAB10 with N-terminal GFP tag

    Depositor

    Michael Bassik

    Article

    Jeng et al Cell Host Microbe. 2019 Oct 9;26(4):551-

    Insert

    RAB10 (RAB10 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. MSCV-human HoxB4-IRES-YFP

    Plasmid

    #91889

    Purpose

    Retroviral expression of human HoxB4

    Depositor

    Brian Sorrentino, Martine Roussel

    Article

    Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

    Insert

    HoxB4 (HOXB4 Human)
    

    Use

    Retroviral

    Tags

    IRES-EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    MMLV gag

    Available since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. TFORF1062

    Plasmid

    #143751

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NFATC1 (NFATC1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. TFORF1061

    Plasmid

    #143750

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NFATC1 (NFATC1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. CamKII-LGI1-pHmScarlet

    Plasmid

    #185538

    Purpose

    Expresses LGI1-pHmScarlet under the CamKII promoter

    Depositor

    Jaime de Juan-Sanz

    Article

    Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

    Insert

    LGI1 (Lgi1 Rat)
    

    Use

    Lentiviral

    Tags

    pHmScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    CamKII

    Available since

    May 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCas9-Geminin

    Plasmid

    #109401

    Purpose

    Expresses Cas9 fusion to Geminin degron domain

    Depositor

    Jean-Paul Concordet

    Article

    Charpentier et al Nat Commun. 2018 Mar 19;9(1):1133. doi:

    Insert

    Geminin (GMNN Human)
    

    Use

    Tags

    Expression

    Mutation

    Geminin degron fragment

    Promoter

    Available since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLKO.1-shCELF4-4405

    Plasmid

    #115466

    Purpose

    Constitutive lentiviral expression

    Depositor

    William Hahn

    Article

    Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

    Insert

    shCELF4-4405 (CELF4 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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