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pCMMP-MCS-IRES-Puro

Plasmid

#36952

Depositor

Bill Sugden

Publication

Lee et al Blood. 2008 Feb 15;111(4):2280-9. Epub 2

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available since

May 16, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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FDP-P10B2-A75-RZ-URA3

Plasmid

#130874

Purpose

Integration cassette for OrthoRep containing mKate driven by promoter 10B2 with a hardcoded polyA tail

Depositor

Chang Liu

Publication

Zhong et al ACS Synth Biol. 2018 Dec 21;7(12):2930-2

Insert

mKate

Use

Expression of mkate, leu2(q180*), and ura3 on ort…

Available since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-hsyn-GRAB_rDA-mut

Plasmid

#140558

Purpose

Expresses the red fluorescent DA-insensitive control sensor GRAB_rDA-mut in neurons

Depositor

Yulong Li

Publication

Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

Has service

AAV9

Insert

Red fluorescent DA-insensitive sensor GRAB_rDA-mut (control sensor)

Use

AAV

Promoter

hSyn

Available since

July 29, 2020

Availability

Academic Institutions and Nonprofits only

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pEB2-E2-Crimson

Plasmid

#104010

Purpose

Plasmid encoding E2-Crimson

Depositor

Philippe Cluzel

Publication

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

E2-Crimson

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to DsRed (R2D, S4T, K5E, E10P,…

Promoter

proC

Available since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pEB1-mVenus NB

Plasmid

#103986

Purpose

Plasmid encoding mVenus NB

Depositor

Philippe Cluzel

Publication

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mVenus NB

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F46L, F64L, …

Promoter

proC

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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FlagMBNL3

Plasmid

#96901

Purpose

expression of Flag-tagged human MBNL3

Depositor

Thomas Cooper

Publication

Orengo et al Nucleic Acids Res. 2006;34(22):e148. Epu

Insert

human MBNL3 cDNA (MBNL3 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pLysscys255

Plasmid

#36429

Depositor

Emine Gunhan, Pete Lollar

Publication

Gunhan et al Protein Expr Purif. 2008 Apr;58(2):203-9

Insert

Saporin-3/cys255

Expression

Bacterial

Mutation

cys added at position 255

Promoter

T7

Available since

May 10, 2012

Availability

Academic Institutions and Nonprofits only

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GFP-JNK1

Plasmid

#86830

Purpose

mammalian expression of GFP-JNK1

Depositor

Rony Seger

Publication

Zehorai et al Mol Cell Biol. 2014 Jan;34(2):259-70. do

Insert

JNK1 (MAPK8 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pBS_12x-601_MA1

Plasmid

#114358

Purpose

12x Widom 601 nucleosome sequence

Depositor

Scot Wolfe

Publication

Swygert et al Proc Natl Acad Sci U S A. 2018 Dec 4;115

Insert

12X601-MA1

Use

Unspecified

Expression

Mammalian

Available since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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Renilla luciferase-Pol III

Plasmid

#37380

Depositor

Norbert Perrimon

Publication

Nybakken et al Nat Genet. 2005 Dec;37(12):1323-32. Epub

Insert

PolIII-Renilla control reporter (RpIII128 Fly)

Use

Luciferase

Expression

Mammalian

Promoter

RNA PolIII 128 subunit (RpIII128)

Available since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles
pNkx-GFP

Plasmid

#45497

Depositor

Sean Wu

Publication

Wu et al Cell. 2006 Dec 15;127(6):1137-50. Epub 2

Insert

2.5kb mouse Nkx2.5 enhancer and promoter fragment (Nkx2-5 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

2.5kb Nkx2.5 enhancer and promoter fragment

Available since

Aug. 22, 2013

Availability

Academic Institutions and Nonprofits only

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1105-Desmoglein2-FLAG-eGFP

Plasmid

#36989

Depositor

Kathleen Green

Publication

Klessner et al Mol Biol Cell. 2009 Jan;20(1):328-37. Ep

Insert

Dsg2 (DSG2 Human)

Tags

FLAG and eGFP

Expression

Mammalian

Promoter

CMV

Available since

June 5, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRluc-LC3wt

Plasmid

#105002

Purpose

Mammalian expression of Rluc-LC3wt. Can be used for measuring autophagic flux.

Depositor

Marja Jããttelã

Publication

Farkas et al Methods Enzymol. 2017;588:1-13. doi: 10.

Insert

LC3B (Map1lc3b Rat)

Use

Luciferase

Tags

Rluc with C124A substitution

Expression

Mammalian

Available since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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LVXN-Neo-NSD2-E1099K

Plasmid

#86011

Purpose

express NSD2 E1099K mutant in mammalian cells

Depositor

Darrin Stuart

Publication

Jaffe et al Nat Genet. 2013 Nov;45(11):1386-91. doi:

Insert

NSD2 E1099K mutant (NSD2 Human)

Tags

Flag tag D Y K D D D D K

Expression

Mammalian

Mutation

E1099K; K1002R (please see depositor comments bel…

Promoter

CMV

Available since

Jan. 13, 2017

Availability

Academic Institutions and Nonprofits only
pECJ3

Plasmid

#75465

Purpose

Toggle switch plasmid containing lacI-pdt3

Depositor

James Collins

Publication

Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

Insert

lacI-pdt3

Expression

Bacterial

Promoter

pLtetO

Available since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRluc-LC3G120A

Plasmid

#105003

Purpose

Mammalian expression of Rluc-LC3G120A. Can be used for measuring autophagic flux.

Depositor

Marja Jããttelã

Publication

Farkas et al Methods Enzymol. 2017;588:1-13. doi: 10.

Insert

LC3B (Map1lc3b Rat)

Use

Luciferase

Tags

Rluc with C124A substitution

Expression

Mammalian

Mutation

Glycine 120 mutated to alanine, prevents lipidati…

Available since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pmyc-TNRC6B

Plasmid

#10978

Depositor

Thomas Tuschl

Publication

Meister et al Curr Biol. 2005 Dec 6;15(23):2149-55. do

Insert

TNRC6B (TNRC6B Human)

Tags

myc

Expression

Mammalian

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV.GFAP.SF-iGluSnFR.A184S

Plasmid

#106192

Purpose

Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Publication

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Has service

AAV1

Insert

SF-iGluSnFR.A184S

Use

AAV

Mutation

GltI: A184S

Promoter

GFAP

Available since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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GST-PRMT6

Plasmid

#34700

Depositor

Steven Clarke

Publication

Frankel et al J Biol Chem. 2002 Feb 1;277(5):3537-43.

Insert

PMRT6

Tags

GST

Expression

Bacterial

Promoter

tac

Available since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only
pEB1-SCFP3A

Plasmid

#103970

Purpose

Plasmid encoding SCFP3A

Depositor

Philippe Cluzel

Publication

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

SCFP3A

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F64L, S65T, …

Promoter

proC

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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