We narrowed to 42,127 results for: Eras
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Plasmid#173430Purposeprotein expression plasmid of 2XSmBiT-SARS-CoV-2 S1-IgG1 FcDepositorInsert2XSmBiT SARS-CoV-2 S1-IgG1 Fc (S SARS-CoV-2)
UseLuciferaseTagshuman IgG1 FcExpressionMammalianMutationfurin cleavage site RRAR to GSASPromoterCMVAvailable SinceJan. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD+7R+12D
Plasmid#178678PurposeBacterial expression of N-terminally 6His tagged A1-LCD with seven Arg residues added, twelve Glu residues added (LCD: low-complexity domain, aa 186-320)DepositorInsertA1-LCD+7R+12D (HNRNPA1 Human)
Tags6xHisExpressionBacterialMutationseven Arg residues added, twelve Glu residues add…Available SinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-I-Sce1-eno2:Tau-IRES-egfp
Plasmid#112205PurposeUsed to make transgenic zebrafish expressing human 4R/0N Tau under the neuronal eno2 promoterDepositorInsertMAPT (MAPT Human)
UseZebrafish expressionTagseno2 exon 2 translational fusion, ires-egfp at c …Promotereno2 from zebrafishAvailable SinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
FGFR1_HUMAN_D0
Plasmid#79719PurposeThis plasmid encodes the kinase domain of FGFR1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL3-noSV40-humanEC1.25_S3-4-5-6
Plasmid#173956PurposeFirefly luciferase enhancer reporter plasmid.DepositorInsertEnhancer regions 3-6 (S3, S4, S5 and S6) from SOX9 enhancer cluster EC1.25
UseLuciferaseMutationNonePromoterSV40 promoterAvailable SinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
lentiMutB3GALT5-blast
Plasmid#208381Purposelentiviral vector for expressing human B3GALT5 with C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequenceDepositorInsertbeta-1,3-galactosyltransferase 5 (B3GALT5 Human)
UseLentiviralTagsmyc, FLAGExpressionMammalianMutationnucleotide change in PAM targeting sequence nt 51…PromoterEF-1 alpha core promoterAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pL452(hygro)-Sf3b1-K700K
Plasmid#90426Purposeselectable HDR vector to introduce silent mutation (position 700) in mus Sf3b1 gene (for use with pL452-Sf3b1-K700E and sgSf3b1(T1) gRNA vectors enabling generation of hemizygous K700E mutation)DepositorInsertSf3b1 - left and right homology arms (Sf3b1 Mouse)
UseHomology-directed repair vectorAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
RP1B FUS 1-163 S12xQ
Plasmid#127193PurposeProtein expression for affinity purificationDepositorInsertFUS 1-163 S12xQ (FUS Human)
TagshexahistidineExpressionBacterialMutationS12xQ: S30Q, S44Q, S48Q, S53Q, S70Q, S84Q, S89Q, …Available SinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBJG1 T7-8xHis-OGT 5N5A
Plasmid#154280PurposeBacterial expression of full length human OGT with 5N5A TPR mutations (inefficient substrate binding). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.DepositorInsertO-GlcNAc Transferase (OGT Human)
Tags8x His, HRV3C protease site, and T7 leaderExpressionBacterialMutationN322A, N356A, N390A, N424A, N458A mutations; bact…Available SinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCCL-PGK-SPdCas9-BFP-DNMT3A(E752A)
Plasmid#71213PurposedCas9 fused to BFP and the human DNMT3A catalytically inactive domainDepositorInsertdSpCas9-BFP-DNMT3A(E752A), DNMT3A catalytic domain with inactivation mutation E752A
TagsdSpCas9-BFP-DNMT3A(E752A)ExpressionMammalianAvailable SinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only