Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 4,245 results for: Pet2

Showing: 3641 - 3680 of 4245 results

  4. Fast-GCaMP-EF16

    Plasmid
    #52642
    Purpose
    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity
    Depositor
    Samuel Wang
    Article
    Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.
    Insert
    Fast-GCaMP-EF16
    Use
    Tags
    Expression
    Mammalian
    Mutation
    D431N/D433N/D435N/E442A mutated from GCaMP3
    Promoter
    T7
    Available since
    April 23, 2014
    Availability
    Academic Institutions and Nonprofits only

  5. Fast-GCaMP-EF19

    Plasmid
    #52644
    Purpose
    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity
    Depositor
    Samuel Wang
    Article
    Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.
    Insert
    Fast-GCaMP-EF19
    Use
    Tags
    Expression
    Mammalian
    Mutation
    T328D/T330D mutated from GCaMP3
    Promoter
    T7
    Available since
    April 23, 2014
    Availability
    Academic Institutions and Nonprofits only

  10. pET:GACT:GFP

    Plasmid
    #60734
    Purpose
    A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GACT' between -11 and -8.
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Insert
    GFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 5, 2014
    Availability
    Academic Institutions and Nonprofits only
    60734-plasmid-map-sequence-id-97720

    Enlarge

  12. SENP2M497A

    Plasmid
    #61088
    Purpose
    cleave the SUMO off when use PETSUMO vector for express fusion protein
    Depositor
    Cynthia Wolberger
    Article
    Wiener et al Nat Struct Mol Biol. 2013 Sep;20(9):1033
    Insert
    SENP2M497A catalytic domain (SENP2 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    M497A; Catalytic domain is residues 364-589
    Promoter
    T7
    Available since
    Jan. 29, 2015
    Availability
    Academic Institutions and Nonprofits only

  18. CXXC1

    Plasmid
    #32045
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CXXC1 (CXXC1 Human)
    Use
    Tags
    His (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    CXXC domain (amino acids residues 161-222)
    Promoter
    Available since
    Jan. 3, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  19. DNMT3B (3QKJ)

    Plasmid
    #32044
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    DNMT3B (DNMT3B Human)
    Use
    Tags
    His (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    PWWP domain only (amino acids 293-442)
    Promoter
    Available since
    Feb. 22, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  22. pET:GACG:GFP

    Plasmid
    #60735
    Purpose
    A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GACG' between -11 and -8.
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Insert
    GFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 5, 2014
    Availability
    Academic Institutions and Nonprofits only
    60735-plasmid-map-sequence-id-97721

    Enlarge

  23. HDGF2

    Plasmid
    #32021
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    HDGF2 (HDGFL2 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    PWWP domain only (amino acid residues 1-93)
    Promoter
    T7
    Available since
    Jan. 3, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  24. pET:GCAT:GFP

    Plasmid
    #60736
    Purpose
    A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GCAT' between -11 and -8.
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Insert
    GFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 5, 2014
    Availability
    Academic Institutions and Nonprofits only
    60736-plasmid-map-sequence-id-97722

    Enlarge

  26. NmMetQ WT

    Plasmid
    #129647
    Purpose
    Expresses the mature metQ gene from Neisseria meningitides, without the signal sequence
    Depositor
    Douglas Rees
    Article
    Nguyen et al Protein Sci. 2019 Jul 26. doi: 10.1002/p
    Insert
    metQ Gene
    Use
    Tags
    10x Histidine Tag, Enterokinase, and pelB signal …
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Available since
    Sept. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    129647-plasmid-map-sequence-id-254339

    Enlarge

  27. 5KCH (SETDB1)

    Plasmid
    #101881
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SETDB1 (SETDB1 Human)
    Use
    Tags
    6xHis, TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 30, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    101881-plasmid-map-sequence-id-194709

    Enlarge

  28. pET:ACAT:GFP

    Plasmid
    #60740
    Purpose
    A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'ACAT' between -11 and -8.
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Insert
    GFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 5, 2014
    Availability
    Academic Institutions and Nonprofits only
    60740-plasmid-map-sequence-id-97725

    Enlarge

  29. PHF13 (3O70)

    Plasmid
    #28154
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PHD finger protein 13 (PHF13 Human)
    Use
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    PHD-type zinc finger domain (residues 232-281).
    Promoter
    Available since
    Feb. 28, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  35. Fast-GCaMP-RS15

    Plasmid
    #52652
    Purpose
    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity
    Depositor
    Samuel Wang
    Article
    Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.
    Insert
    Fast-GCaMP-RS15
    Use
    Tags
    Expression
    Mammalian
    Mutation
    deleted G47/H48 from GCaMP3
    Promoter
    T7
    Available since
    May 6, 2014
    Availability
    Academic Institutions and Nonprofits only

  36. pET:CCCT:GFP

    Plasmid
    #60738
    Purpose
    A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'CCCT' between -11 and -8.
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Insert
    GFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 18, 2014
    Availability
    Academic Institutions and Nonprofits only
    60738-plasmid-map-sequence-id-97724

    Enlarge

  37. MALT1-DD (2G7R)

    Plasmid
    #25613
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    MALT1-DD (MALT1 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains Death Domain
    Promoter
    Available since
    Aug. 31, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 3641 - 3680 of 4245 results