We narrowed to 5,633 results for: chia

1. pNP2157

   Plasmid

   #222732

   Purpose

   pNP1950 - J23119-IS621 bRNA TBL-WT, DBL-WT, HDV; T7-IS621 Recombinase

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

   Insert

   IS621 Helper (WT)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBEL1284

   Plasmid

   #175960

   Purpose

   For expression of LetA-LetB operon from E. coli. N-terminal his tag on LetA, araBAD promoter

   Depositor

   Damian Ekiert, Gira Bhabha

   Article

   Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

   Inserts

   LetA
   LetB
   

   Use

   Tags

   6xHis 2xQH and TEV cleavage site on LetA

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1 MCS-BirA(R118G)-HA

   Plasmid

   #36047

   Purpose

   To fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); HA tag

   Depositor

   Kyle Roux

   Article

   Roux et al J Cell Biol. 2012 Mar 12.

   Has Service

   Cloning Grade DNA

   Insert

   BirA(R118G)-HA
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   R118G (highly promiscuous form)

   Promoter

   Available since

   April 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pNP568

   Plasmid

   #222725

   Purpose

   pNP555 - IS621 RE-LE TBL-WT, DBL-WT bRNA in cis

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

   Insert

   IS621 bridge RNA
   

   Use

   Tags

   sfGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBMN YFP-DHFR(DD)

   Plasmid

   #29326

   Depositor

   Thomas Wandless

   Article

   Iwamoto et al Chem Biol. 2010 Sep 24. 17(9):981-8.

   Insert

   YFP-DHFR(DD)
   

   Use

   Retroviral

   Tags

   HA and YFP

   Expression

   Mutation

   Change Arg 12 to His (R12H),Asn 18 Thr (N18T), Va…

   Promoter

   Available since

   April 1, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET-MCN-10His-mStayGold (E138D)

   Plasmid

   #211361

   Purpose

   Bacterial expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Codon optimised for expression in Escherichia coli. Contains a N-terminal 10xHis tag.

   Depositor

   Mohan Balasubramanian

   Article

   Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

   Insert

   mStayGold
   

   Use

   Tags

   10xHis-tag

   Expression

   Bacterial

   Mutation

   E138D

   Promoter

   T7

   Available since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pNP1830

   Plasmid

   #222728

   Purpose

   pNP561 - T5 Target with T7 Inducible IS621 Recombinase

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

   Insert

   IS621 Recombinase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTP264

   Plasmid

   #149334

   Purpose

   Bacterial expression of Biotin ligase BirA

   Depositor

   Dirk Görlich

   Article

   Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554

   Insert

   Biotin ligase BirA
   

   Use

   Tags

   His14 and bdNEDD8

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pNP1047

   Plasmid

   #222726

   Purpose

   pNP555 - IS621 RE-LE TBL-T5, DBL-WT bRNA in cis

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

   Insert

   IS621 bridge RNA
   

   Use

   Tags

   sfGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. secreted BirA-Flag

    Plasmid

    #64395

    Purpose

    Flag-tagged biotinylation enzyme for co-expression with bio-proteins

    Depositor

    Gavin Wright

    Article

    Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub

    Insert

    BirA (birA E. coli)
    

    Use

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pOxyR_gfp

    Plasmid

    #194154

    Purpose

    Green Fluorescent Reporter of intracellular hydrogen peroxide

    Depositor

    Sven Panke

    Article

    Kardashliev et al Chem Sci. 2021 Oct 29;12(44):14766-14772

    Insert

    transcriptional regulator, OxyR, cognate promoter PoxyS, fluorescent protein sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PoxyR, PoxyS

    Available since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pNP1954

    Plasmid

    #222730

    Purpose

    pNP1723 - IS621 WT Donor and bridgeRNA in trans (TBL-T5, DBL-WT)

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

    Insert

    IS621 bridge RNA
    

    Use

    Tags

    sfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1 mycBioID

    Plasmid

    #35700

    Purpose

    To fuse your protein of interest to the C-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); myc tag

    Depositor

    Kyle Roux

    Article

    Roux et al J Cell Biol. 2012 Mar 12.

    Has Service

    Cloning Grade DNA

    Insert

    BirA
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    R118G (highly promiscuous form)

    Promoter

    cmv

    Available since

    April 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBbA5c-MevT-MBIS

    Plasmid

    #35150

    Depositor

    Taek Soon Lee, Jay Keasling

    Article

    Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

    Insert

    AtoB(o)-HMGS(o)-HMGR(o)-MK(o)-PMK(o)-PMD-Idi-IspA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    LacUV5,

    Available since

    March 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Nuclear PancACe

    Plasmid

    #215706

    Purpose

    Expresses acetyl-CoA biosensor in the nucleus

    Depositor

    Katharine Diehl

    Article

    Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

    Insert

    PancACe
    

    Use

    Lentiviral

    Tags

    FLAG, HA, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSR83

    Plasmid

    #90211

    Purpose

    Expresses the response regulator OmpR, and expresses mCherry from the Cph8-OmpR regulated PompF-146 promoter

    Depositor

    Jeffrey Tabor

    Article

    Olson et al Mol Syst Biol. 2017 Apr 24;13(4):926. do

    Inserts

    OmpR (ompR E. coli)
    mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEvoltac-EcW-TGA-h14

    Plasmid

    #218764

    Purpose

    encodes EcWRS-h14 and EcW-tRNA-TGA

    Depositor

    Abhishek Chatterjee

    Article

    Italia et al Nat Chem Biol. 2017 Apr;13(4):446-450. d

    Insert

    EcWRS-h14
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    S8A, V144G, V146C

    Promoter

    tacI

    Available since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET-MCN-10His-StayGold

    Plasmid

    #211360

    Purpose

    Bacterial expression of the bright and photostable StayGold fluorescent protein. Codon optimised for expression in Escherichia coli. Contains a N-terminal 10xHis tag.

    Depositor

    Mohan Balasubramanian

    Article

    Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

    Insert

    StayGold
    

    Use

    Tags

    10xHis-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pIRE4-Bpa

    Plasmid

    #155342

    Purpose

    Plasmid encoding the translational pair that enables efficient incorporation of Benzoyl-phenylalanine in response to the amber codon into proteins in mammmalian cells.

    Depositor

    Irene Coin

    Article

    Bottke et al EMBO Rep. 2020 Sep 14:e50437. doi: 10.15

    Insert

    humanized EBpaRS/BstYam
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK/U6

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pVS2

    Plasmid

    #33144

    Depositor

    Michael Rosbash

    Article

    Vodala et al Mol Cell. 2008 Jul 11;31(1):104-13.

    Inserts

    LacO (256 copies)
    GAL-GFP-kd200-GALpA reporter
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    GFP is mutated such that amino acids 64-73 (FSYGV…

    Promoter

    bidirectional GAL1-GAL10 promoter

    Available since

    Dec. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

21. p201N Cas9

    Plasmid

    #59175

    Purpose

    Cas9 driven by double 35S, nptII for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

    Depositor

    Wayne Parrott

    Article

    Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

    Inserts

    Cas9
    nptII
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    2x35S and StUbi-3P

    Available since

    Sept. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. SpyTag-β-Lactamase-SpyCatcher

    Plasmid

    #52656

    Purpose

    Expresses SpyTag-SpyCatcher mediated cyclized version of TEM-1 β-lactamase (BLA). Cyclization of BLA led to an increase in thermal resilience of >60 degrees celsius.

    Depositor

    Mark Howarth

    Article

    Schoene et al Angew Chem Int Ed Engl. 2014 Jun 10;53(2

    Insert

    TEM-1 β-Lactamase
    

    Use

    Tags

    SpyCatcher and SpyTag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    May 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEF1a-BirA-V5-neo

    Plasmid

    #100548

    Purpose

    Expresses C-terminal V5-tagged BirA biotin ligase

    Depositor

    Jian Xu

    Article

    Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.

    Insert

    C-terminal V5-tagged BirA
    

    Use

    Tags

    V5, His

    Expression

    Mammalian

    Mutation

    Promoter

    Human EF1a

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET30a-AlkB-D135S

    Plasmid

    #79051

    Purpose

    Express mutant AlkB protein (D135S) in E. coli BL21(DE3)

    Depositor

    Tao Pan

    Article

    Zheng et al Nat Methods. 2015 Sep;12(9):835-7. doi:

    Insert

    AlkB (alkB E.coli)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    A truncated AlkB with deletion of the amino (N)-t…

    Promoter

    T7

    Available since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET30a-AlkB

    Plasmid

    #79050

    Purpose

    Express wild type AlkB protein in E. coli BL21(DE3)

    Depositor

    Tao Pan

    Article

    Zheng et al Nat Methods. 2015 Sep;12(9):835-7. doi:

    Insert

    AlkB (alkB E.coli)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    A truncated AlkB with deletion of the amino (N)-t…

    Promoter

    T7

    Available since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSIM24-GUS

    Plasmid

    #67882

    Purpose

    Plant Gene Expression Vector pSIM24 containing the GUS (Beta-glucuronidase) reporter gene

    Depositor

    Indu Maiti

    Article

    Sahoo et al PLoS One. 2014 Jun 4;9(6):e98988. doi: 1

    Insert

    uidA
    

    Use

    Tags

    Expression

    Plant

    Mutation

    T120A

    Promoter

    M24

    Available since

    Aug. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSLS.402

    Plasmid

    #187202

    Purpose

    Expresses retron Eco1 RT

    Depositor

    Seth Shipman

    Article

    Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

    Insert

    Retron Eco1 Reverse Transcriptase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    mphR

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET15bpATnp

    Plasmid

    #121137

    Purpose

    This vector encode a His6-tagged proteinA-Tnp(hyperactive form) fusion protein.

    Depositor

    Keji Zhao

    Article

    Carter et al Nat Commun. 2019 Aug 20;10(1):3747. doi:

    Insert

    Protein A-Tnp
    

    Use

    Tags

    his

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSLS.492

    Plasmid

    #184957

    Purpose

    Test effect of a1/a2 length on editing rpoB using retron recombineering

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 RT and recombineering ncRNA, rpoB T1534C, a1/a2 length: 22
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    rpoB donor T1534C, a1/a2 length extended to 22 bp

    Promoter

    T7/lac

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMS82

    Plasmid

    #68110

    Purpose

    Integration vector derived from Streptomyces phage phiBT1. Hygromycin resistance gene, oriT, ColEi origin of replication

    Depositor

    Maggie Smith

    Article

    Gregory et al J Bacteriol. 2003 Sep;185(17):5320-3.

    Inserts

    aac3(IV)
    Hyg
    oriT
    attP
    int
    

    Use

    Streptomyces integrating vector

    Tags

    Expression

    Mutation

    Fragment

    Promoter

    Available since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pG108-K - AhpC-Glow

    Plasmid

    #185135

    Purpose

    Bacteroides - Escherichia coli shuttle vector with TetQ and Kanamycin selection markers BS2 is cloned under the Bacteroides thetaiotaomicron ahpC promoter

    Depositor

    Janina Lewis

    Article

    Jones et al Mol Oral Microbiol. 2020 Aug;35(4):181-1

    Inserts

    Glow gene
    Bacteroides thetaiotaomicron ahpC promoter
    

    Use

    Pg106

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pSHDY-Prha-mVenus_rhaS

    Plasmid

    #137662

    Purpose

    Conjugative vector derived from pSHDY. Constitutive expression of the activator rhaS, as well as rhamnose-inducible expression of mVenus.

    Depositor

    Ilka Axmann

    Article

    Behle et al ACS Synth Biol. 2020 Apr 17;9(4):843-855

    Insert

    Prha:mVenus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    rhaS (Arg218Leu)

    Promoter

    Prha

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCT5-bac1.8

    Plasmid

    #119871

    Purpose

    Constitutive expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coli

    Depositor

    Claudia Schmidt-dannert

    Article

    Seo et al Appl Microbiol Biotechnol. 2018 Nov 3. p

    Inserts

    CymR'
    sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    SNP in 592 bp

    Promoter

    PserA promoter and Pveg promoter with CuO sequence

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pOZITX-S1

    Plasmid

    #184925

    Purpose

    Expresses the catalytic S1 subunit of OZITX (EcPltAB)

    Depositor

    Jonathan Javitch

    Article

    Keen et al Commun Biol. 2022 Mar 23;5(1):256. doi:

    Insert

    codon optimized S1 subunit of OZITX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pHCKan-phoAp-GFPuv

    Plasmid

    #139072

    Purpose

    Low phosphate inducible promoter phoAp for GFP expression

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2020 Jun 19;9(6):1483-14

    Insert

    phoAp-GFPuv
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pBES1-LacOx50-Hyg

    Plasmid

    #210261

    Purpose

    Integrates 50xLacO repeats upstream of the BES1 promoter with a hygromycin drug selection marker downstream of the promoter.

    Depositor

    Calvin Tiengwe

    Article

    Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

    Insert

    50xLacO repeats derived from: PMID: 12864855.
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pBLADE_ONLY_C

    Plasmid

    #168050

    Purpose

    Expresses the synthetic gene BLADE FP6 under the constitutive J23101** promoter.

    Depositor

    Barbara Di Ventura

    Article

    Romano et al Nat Chem Biol. 2021 Apr 26. pii: 10.1038

    Insert

    Blue light-inducible AraC dimers in Escherichia coli
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23101**

    Available since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pBMN DHFR(DD)-YFP

    Plasmid

    #29325

    Depositor

    Thomas Wandless

    Article

    Iwamoto et al Chem Biol. 2010 Sep 24. 17(9):981-8.

    Insert

    DHFR(DD)-YFP
    

    Use

    Retroviral

    Tags

    HA and YFP

    Expression

    Mutation

    Change Arg 12 to Tyr (R12Y),Gly 67 to Ser (G67S) …

    Promoter

    Available since

    July 1, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pBLADE_ONLY_A

    Plasmid

    #168051

    Purpose

    Expresses the synthetic gene BLADE FP6 under the constitutive J23101** promoter.

    Depositor

    Barbara Di Ventura

    Article

    Romano et al Nat Chem Biol. 2021 Apr 26. pii: 10.1038

    Insert

    Blue light-inducible AraC dimers in Escherichia coli
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23101**

    Available since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. p201H Cas9

    Plasmid

    #59176

    Purpose

    Cas9 driven by double 35S, hygromycin resistance for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

    Depositor

    Wayne Parrott

    Article

    Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

    Inserts

    Cas9
    hph
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    2x35S and StUbi-3P

    Available since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pHCKan-yibDp2-GFPuv

    Plasmid

    #139083

    Purpose

    Low phosphate inducible promoter yibDp2 for GFP expression

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2020 Jun 19;9(6):1483-14

    Insert

    yibDp2-GFPuv
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pXD70LacZ1-promoterless-LacZ

    Plasmid

    #191617

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), promoterless-lacZ

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    lacZ
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pTXRE5-Z

    Plasmid

    #112509

    Purpose

    Reporter plasmid, responsive to activated Ah receptor/Arnt

    Depositor

    Charles Miller

    Article

    Miller CA et al Toxicol Appl Pharmacol. 1999 Nov 1;160(3

    Insert

    RW95-3 with 5x xenobiotic response elements (lacZ Synthetic, E.coli)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    5 XREs+ minimal Cytochrome C

    Available since

    July 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. mCherry-eDHFR

    Plasmid

    #107266

    Purpose

    mCherry-eDHFR in pIVT vector for in vitro transcription

    Depositor

    Michael Lampson

    Article

    Akera et al Science. 2017 Nov 3;358(6363):668-672. d

    Insert

    mCherry-eDHFR (dhfr E.coli)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pMA2640

    Plasmid

    #25434

    Purpose

    Retroviral Tet-On vector for CMV-driven rtTA with EGFP and Blasticidin selection

    Depositor

    Mikhail Alexeyev

    Article

    Alexeyev et al Mol Biol Rep. 2010 Apr . 37(4):1987-91.

    Insert

    reverse tetracycline-regulated transactivator Advanced
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. Dead-Laconic/pcDNA3.1(-)

    Plasmid

    #118627

    Purpose

    To express in mammalian cells a lactate FRET sensor negative control.

    Depositor

    Luis Felipe Barros

    Article

    Baeza-Lehnert et al Methods Mol Biol. 2020;2184:19-30. doi:

    Insert

    Dead-Laconic
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Change Histidine 151 to Aspartic Acid of Escheric…

    Promoter

    CMV Promoter

    Available since

    Dec. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. Traptavidin-E6

    Plasmid

    #59549

    Purpose

    pET21-Tre for expression of traptavidin (high stability mutant of streptavidin) bearing a glutamic acid tag for ion-exchange chromatography

    Depositor

    Mark Howarth

    Article

    Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-

    Insert

    Core Traptavidin-E6
    

    Use

    Tags

    E6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

48. Skp

    Plasmid

    #213139

    Purpose

    Expresses chaperone protein SKP

    Depositor

    Sebastian Hiller

    Article

    Rath et al Nat Commun. 2023 Sep 13;14(1):5648. doi:

    Insert

    SKP (21-161) (skp E.coli)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pET28a_T7-ARG2

    Plasmid

    #106474

    Purpose

    Expresses acoustic reporter genes or gas vesicles (ARG2) in Escherichia coli

    Depositor

    Mikhail Shapiro

    Article

    Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

    Insert

    ARG2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pAR357_SFP_pCDF-1b

    Plasmid

    #122851

    Purpose

    Co-expresses the phosphopantheteine transferase Sfp of Bacillus subtilis to transfer a PPant arm to acyl carrier proteins in Escherichia coli. Untagged; in pCDF-1b vector.

    Depositor

    Martin Grininger

    Article

    Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

    Insert

    Sfp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge