We narrowed to 15,981 results for: LEA

1. Tol2_B-act_dCas9-Vp64-T2A-Citrine

   Plasmid

   #200248

   Purpose

   Zebrafish transgenics; Plasmid encodes dCas9-Vp64 and could be stably integrated in the zebrafish genome using Tol2 transgenesis

   Depositor

   Tatjana Sauka-Spengler

   Article

   Pelea et al bioRxiv 2023.05.08.539738

   Insert

   dCas9-Vp64-T2A-Citrine
   

   Use

   Zebrafish expression

   Available Since

   July 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-ZFN-AAVS1

   Plasmid

   #89707

   Purpose

   Mammalian expression plasmid for zinc finger nuclease targeting human AAVS1 locus

   Depositor

   Dmitriy Mazurov

   Article

   Zotova et al Viruses. 2017 Nov 2;9(11). pii: v9110325

   Insert

   ZFN-AAVS1
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDH-NLS-copGFP-EF1-BlastiS

   Plasmid

   #132772

   Purpose

   Spills into the cytoplasm during nuclear envelope rupture.

   Depositor

   Jan Lammerding

   Article

   Denais et al Science. 2016 Apr 15;352(6283):353-8. do

   Insert

   NLS-copGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL3-mArg1 promoter/enhancer -31/-3810

   Plasmid

   #34571

   Depositor

   Peter Murray

   Article

   Pauleau et al J Immunol. 2004 Jun 15;172(12):7565-73.

   Insert

   Arg1 promoter/enhancer -31/-3810 (Arg1 Mouse)
   

   Use

   Luciferase

   Tags

   Firefly Luciferase

   Available Since

   Jan. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. SpyTag-sfGFP

   Plasmid

   #184226

   Purpose

   Expresses SpyTag-superfolder GFP in the bacterial cytoplasm. SpyTag enables covalent reaction with SpyCatcher.

   Depositor

   Mark Howarth

   Article

   Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

   Insert

   SpyTag-sfGFP
   

   Tags

   His6, SpyTag, and TEV protease cleavage site

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA-NES-PhoCl-mCherry

   Plasmid

   #87690

   Purpose

   Express NES-PhoCl-mCherry in mammalian cells

   Depositor

   Robert Campbell

   Article

   Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

   Insert

   NES-PhoCl-mCherry
   

   Tags

   nuclear export signal

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGL3-mArg1 base promoter -31/-2365

   Plasmid

   #34570

   Depositor

   Peter Murray

   Article

   Pauleau et al J Immunol. 2004 Jun 15;172(12):7565-73.

   Insert

   Arg1 base promoter -31/-2365 (Arg1 Mouse)
   

   Use

   Luciferase

   Tags

   Firefly Luciferase

   Available Since

   Dec. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pAcGFP-MALAT1M

   Plasmid

   #120382

   Purpose

   Reporter for RNA nuclear localization

   Depositor

   Igor Ulitsky

   Article

   Lubelsky et al Nature. 2018 Mar 1;555(7694):107-111. do

   Insert

   MALAT1 (MALAT1 Human)
   

   Tags

   AcGFP1

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHsp70-Cas9

   Plasmid

   #45945

   Purpose

   The codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter used in Gratz, et al. (2013). Plasmid is low copy number.

   Depositor

   Kate O'Connor-Giles, Melissa Harrison, Jill Wildonger

   Article

   Gratz et al Genetics. 2013 May 24.

   Insert

   Codon optimized Cas9
   

   Use

   CRISPR

   Tags

   3x FLAG

   Mutation

   Silent change from C to A at bp 834 of insert

   Available Since

   July 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTetOne-tdTomato-cNLS

    Plasmid

    #138529

    Purpose

    Inducible expression of tdTomato with nuclear localization signal

    Depositor

    Christopher Grigsby, Ana Teixeira

    Article

    Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

    Insert

    tdTomato
    

    Tags

    3x SV40 cNLS

    Expression

    Mammalian

    Promoter

    TRE3Gs

    Available Since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSQT834

    Plasmid

    #53371

    Purpose

    Csy4 and Cas9 nuclease expression plasmid

    Depositor

    Keith Joung

    Article

    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    Csy4-T2A-Cas9-NLS
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. NET47 pEGFP-N2 (593)

    Plasmid

    #61986

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

    Insert

    NET47 (TM7SF2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pCAG-T3-hCAS-pA

    Plasmid

    #48625

    Purpose

    Expresses CAS9 nuclease in mammalian cells. Used to modify genome of mouse embroys

    Depositor

    Wataru Fujii, Kunihiko Naito

    Article

    Fujii et al Nucleic Acids Res. 2013 Aug 30.

    Insert

    Codon optimized Cas9
    

    Use

    CRISPR

    Tags

    FLAG, NLS, and polyA

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA-NLS-PhoCl-mCherry

    Plasmid

    #87691

    Purpose

    Express NLS-PhoCl-mCherry in mammalian cells

    Depositor

    Robert Campbell

    Article

    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    NLS-PhoCl-mCherry
    

    Tags

    nuclear localization signal

    Expression

    Mammalian

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-mCherry-TSC2

    Plasmid

    #192440

    Purpose

    Encodes mCherry-tagged wild-type TSC2 isoform 5.

    Depositor

    Jin Zhang

    Article

    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

    Insert

    mCherry-TSC2 (TSC2 Human)
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. M-NMn-VP64

    Plasmid

    #48676

    Purpose

    Mammalian NM-VP64 nuclease-null Cas9 activator expression, human optimized

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    Cas9-VP64, nuclease-null
    

    Use

    CRISPR

    Tags

    NLS and VP64

    Mutation

    nuclease-null

    Promoter

    CMV

    Available Since

    Oct. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUB-MSP1D1

    Plasmid

    #161609

    Purpose

    Bacterial expression of mutated membrane scaffold protein ("ultra-bright" MSP1D1) for Nanodisc formation

    Depositor

    Stephen Sligar

    Article

    McLean et al Anal Biochem. 2020 Oct 15;607:113860. do

    Insert

    UD-MSP1D1
    

    Tags

    7-His tag

    Expression

    Bacterial

    Mutation

    mutant of MSP1D1 ( Q138W, L181W, Y236W)

    Promoter

    T7

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCL1032

    Plasmid

    #217962

    Purpose

    Mammalian expression of NASP ('s' isofrom) fused at its N-terminus to eGFP with a TEV protease site separating the two. Puromycin resistance is expressed from a downstream IRES.

    Depositor

    Andrew Bowman

    Article

    Apta-Smith et al EMBO J. 2018 Oct 1;37(19):e98714. doi: 1

    Insert

    NASP (NASP Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. M-ST1n-VP64

    Plasmid

    #48675

    Purpose

    Mammalian ST1-VP64 nuclease-null Cas9 activator expression, human optimized

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    Cas9-VP64, nuclease-null
    

    Use

    CRISPR

    Tags

    NLS and VP64

    Mutation

    nuclease-null (D9A D598A H599A N622A)

    Promoter

    CMV

    Available Since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV-H2GH

    Plasmid

    #182202

    Purpose

    Adeno-associated viral vector to express histone2B-HaloTag-GFP fusion, a nuclear-localized Halotag-GFP fusion construct

    Depositor

    Joshua Kritzer

    Article

    Deprey et al ACS Chem Biol. 2022 Jan 15. doi: 10.1021

    Insert

    histone2B-HaloTag-GFP
    

    Use

    AAV

    Available Since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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