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We narrowed to 12,189 results for: LEA

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Showing: 1821 - 1840 of 12189 results

  1. Stx1a-12aa-mCer

    Plasmid
    #53236
    Purpose
    Fluorescent probe: Syntaxin 1A, C-terminally-tagged with monomeric Cerulean, connected with a 12 amino acid linker. Expressed in mammalian cells, used with SNAP25B-12aa-mCit to measure FRET & dispers.
    Depositor
    Robert Zucker
    Publication
    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.
    Insert
    Syntaxin 1A (Stx1a Rat)
    Tags
    12aa-linker and mCerulean
    Expression
    Mammalian
    Available since
    June 9, 2014
    Availability
    Academic Institutions and Nonprofits only

  6. pET22b_PstS_2

    Plasmid
    #78199
    Purpose
    Expression of double-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor
    Depositor
    Martin Webb
    Publication
    Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62
    Insert
    pstS (A17C,A197C)
    Expression
    Bacterial
    Mutation
    A17C; A197C (please see depositor comment below)
    Promoter
    T7
    Available since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    78199-plasmid-map-sequence-id-146049

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  9. COVID-SARS2 NSP14/10

    Plasmid
    #159613
    Purpose
    Bacterial co-expression vector fo Covid-SARS2 NSP14 and NSP10
    Depositor
    Opher Gileadi
    Publication
    Baddock et al Nucleic Acids Res. 2022 Jan 17. pii: 650
    Inserts
    Covid-SARS2 Nsp14 (ORF1ab COVID-SARS2, Synthetic)
    Covid-SARS2 Nsp10
    Tags
    His6, TEV cleavage site and none
    Expression
    Bacterial
    Mutation
    Codon-optimized for E. coli expression
    Promoter
    (Bicistronic) and T7 - LacO
    Available since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    159613-plasmid-map-sequence-id-311056

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  10. pDNA2.0 6H-TEV-KRAS G12V

    Plasmid
    #111850
    Purpose
    Expresses activated human KRAS G12V (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.
    Depositor
    Kenneth Westover
    Publication
    Hunter et al Mol Cancer Res. 2015 Sep;13(9):1325-35.
    Insert
    KRAS G12V (KRAS Human)
    Tags
    6xHis-tag and TEV protease cleavage sequence
    Expression
    Bacterial
    Mutation
    Changed Glycine 12 to Valine
    Promoter
    T5
    Available since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    111850-plasmid-map-sequence-id-213464

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  11. pcDNA3.1-KozATG-dL5-2XG4S-mCer3

    Plasmid
    #73207
    Purpose
    Expresses dL5(E52D)-mCer3 fusion protein in cytosol of mammalian cells, penetrates nucleus. (MBIC5, dL5**, FAP)
    Depositor
    Marcel Bruchez
    Publication
    Telmer et al ACS Chem Biol. 2015 May 15;10(5):1239-46
    Insert
    KozATG-dL5-2XG4S-mCer3
    Tags
    The FAP and mCerulean3 are fused with 2 copies of…
    Expression
    Mammalian
    Mutation
    The dL5** FAP is E50D, L89S, also known as E52D/L…
    Promoter
    CMV
    Available since
    March 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    73207-plasmid-map-sequence-id-139724

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  15. pLL3.7-EF-EYFP-YAP1_5SA-PolyA

    Plasmid
    #112285
    Purpose
    Fluorescently labels human YAP1-2gamma isoform (504 aa) 5SA active mutant - serines 61, 109, 127, 164 and 397 (also known as 381 in other isoforms)
    Depositor
    Erik Sahai
    Publication
    Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.
    Insert
    EYFP-YAP1 (YAP1 Human)
    Use
    Lentiviral
    Mutation
    Human YAP1-2gamma with 5 Serines (61, 109, 127, 1…
    Promoter
    EF-1 alpha
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    112285-plasmid-map-sequence-id-227437

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  19. pAAV-hSyn-GRAB_g5-HT3.0 (AAV8)

    Viral prep
    #208717-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-hSyn-GRAB_g5-HT3.0 (#208717). In addition to the viral particles, you will also receive purified pAAV-hSyn-GRAB_g5-HT3.0 plasmid DNA. Synapsin-driven expression of GRAB_g5-HT3.0 sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Yulong Li
    Publication
    Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi
    Promoter
    Syn
    Available since
    Sept. 22, 2025
    Availability
    Academic Institutions and Nonprofits only

  20. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV8)

    Viral prep
    #112677-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Brandon Harvey
    Publication
    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3
    Promoter
    EF1a
    Tags
    mCherry (Cre-negative cells), EGFP (Cre-positive cells)
    Available since
    Sept. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
Showing: 1821 - 1840 of 12189 results