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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,562 results for: nls

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Showing: 1821 - 1840 of 6562 results

  1. plenti-Lb-Cas12a-2xNLS

    Plasmid
    #155046
    Purpose
    Lentiviral vector expressing human codon-optimized _C-terminal Myc-tagged Lachnospiraceae bacterium (Lb)-Cas12a nuclease with N- and C-terminal NLS and Neomycin/G418/Geneticin resistance from EF-1alpha promoter
    Depositor
    Benjamin Blencowe, Jason Moffat
    Publication
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    LbCas12a
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS, Myc-tag and SV40 NLS
    Expression
    Mammalian
    Promoter
    EF-1a
    Available since
    Aug. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    155046-plasmid-map-sequence-id-308670

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  5. pSBbi-NLSmCherryNLS Clover-HDHB

    Plasmid
    #190223
    Purpose
    Fluorescent cell cycle reporter
    Depositor
    Laura Heiser
    Publication
    Gross et al Nat Commun. 2023 Jun 10;14(1):3450. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Human DNA Helicase B (amino acids 994-1087) (HELB Human)
    NLS-mCherry-NLS-P2A-Puromycin
    Use
    Transposon
    Tags
    Clover fluorescent protein
    Expression
    Mammalian
    Mutation
    Amino Acids 994-1087
    Promoter
    EF1a and RPBSA
    Available since
    Sept. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    190223-plasmid-map-sequence-id-371298

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  6. DsRed2-Nucleus-4

    Plasmid
    #55839
    Purpose
    Localization: Nucleus, Excitation: 558, Emission: 583
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SV40 T-Antigen Nuclear Localization Signal (NLS)
    Tags
    DsRed2-NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 12, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  8. pCFJ1955

    Plasmid
    #84830
    Purpose
    Minimos transposon with Peft-3:tdTomato:tbb-2 3'UTR and cbr-unc-119 selection. tdTomato was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and synthetic introns
    Depositor
    Andrew Fire
    Publication
    Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10
    Insert
    C. elegans codon optimized tdTomato
    Expression
    Bacterial and Worm
    Promoter
    Peft-3
    Available since
    Dec. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    84830-plasmid-map-sequence-id-163668

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  9. pAL005

    Plasmid
    #233865
    Purpose
    MoClo-YTK URA multigene plasmid with MCS; ABA systems; pTDH3-PhlF-NES-PYL1-tADH1; pTDH3-NLS-VP48-2xOaf1-ABI-tENO1; phlfO7-pLEU2m-MCS-tENO2
    Depositor
    Jeff Hasty
    Publication
    O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102
    Insert
    pTDH3-PhlF-NES-PYL1-tADH1; pTDH3-NLS-VP48-2xOaf1-ABI-tENO1; phlfO7-pLEU2m-MCS-tENO2
    Expression
    Yeast
    Available since
    May 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    233865-plasmid-map-sequence-id-468718

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  12. pBSII-NLS-mEmerald(AM)

    Plasmid
    #244811
    Purpose
    In-Vitro Synthesis of mRNA encoding a Honeybee-optimized mEmerald-labeled Nuclear Localization Signal (NLS) Tag
    Depositor
    Frederic Strobl
    Insert
    mEmerald
    Use
    In-vitro mrna synthesis
    Tags
    SV40 NLS
    Mutation
    coding sequence is optimized for the Honeybee
    Promoter
    T7
    Available since
    Oct. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    244811-plasmid-map-sequence-id-489773

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  13. pBSII-NLS-mTagBFP(AM)

    Plasmid
    #244808
    Purpose
    In-Vitro Synthesis of mRNA encoding a Honeybee-optimized mTagBFP2-labeled Nuclear Localization Signal (NLS) Tag
    Depositor
    Frederic Strobl
    Insert
    mTagBFP2
    Use
    In-vitro mrna synthesis
    Tags
    SV40 NLS
    Mutation
    coding sequence is optimized for the Honeybee
    Promoter
    T7
    Available since
    Oct. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    244808-plasmid-map-sequence-id-489760

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  20. pCFJ1137

    Plasmid
    #84827
    Purpose
    Minimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and synthetic introns
    Depositor
    Andrew Fire
    Publication
    Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10
    Insert
    C. elegans codon optimized GFP
    Expression
    Bacterial and Worm
    Promoter
    Peft-3
    Available since
    Dec. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    84827-plasmid-map-sequence-id-163671

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Showing: 1821 - 1840 of 6562 results