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pX458-Ef1a-dCas9-VPR-H2B-GFP (CRISPRa)

Plasmid

#175571

Purpose

Inactivated Cas9 (dCas9) fusion to VPR for the purpose of targeted activation

Depositor

Joseph Brzezinski

Type

Empty backbone

Use

CRISPR

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-dCas9-VPR-H2B-mCherry (CRISPRa)

Plasmid

#175572

Purpose

Inactivated Cas9 (dCas9) fusion to VPR for the purpose of targeted activation

Depositor

Joseph Brzezinski

Type

Empty backbone

Use

CRISPR

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-dCas9-KRAB-MECP2-H2B-GFP (CRISPRi)

Plasmid

#175573

Purpose

Inactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repression

Depositor

Joseph Brzezinski

Type

Empty backbone

Use

CRISPR

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-dCas9-KRAB-MECP2-H2B-mCherry (CRISPRi)

Plasmid

#175574

Purpose

Inactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repression

Depositor

Joseph Brzezinski

Type

Empty backbone

Use

CRISPR

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_TOMM20-CLIP-tag2

Plasmid

#254210

Purpose

Mamalian cell expression of CLIP-tag2 on mitochondiral surface

Depositor

Kai Johnsson

Article

Johnsson et al bioRxiv 2026.03.05.709795

Insert

TOMM20-CLIP-tag2

Expression

Mammalian

Promoter

CMV

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_H2B-CLIP-tag2

Plasmid

#254207

Purpose

Mamalian cell expression of nuclear CLIP-tag2

Depositor

Kai Johnsson

Article

Johnsson et al bioRxiv 2026.03.05.709795

Insert

H2B-CLIP-tag2

Expression

Mammalian

Promoter

CMV

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_Lifeact-CLIP-tag2

Plasmid

#254208

Purpose

Mamalian cell expression of CLIP-tag2 on actin filaments

Depositor

Kai Johnsson

Article

Johnsson et al bioRxiv 2026.03.05.709795

Insert

Lifeact-CLIP-tag2

Expression

Mammalian

Promoter

CMV

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_Cep41-CLIP-tag2

Plasmid

#254209

Purpose

Mamalian cell expression of CLIP-tag2 associated to microtubules

Depositor

Kai Johnsson

Article

Johnsson et al bioRxiv 2026.03.05.709795

Insert

Cep41-CLIP-tag2

Expression

Mammalian

Promoter

CMV

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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MAC3-tag-N

Plasmid

#185482

Purpose

Gateway compatible destination vectors containing StrepIII/HA/UltralD

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Type

Empty backbone

Tags

StrepIII-HA-UltraID

Expression

Bacterial and Mammalian

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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MAC3-tag-C

Plasmid

#185481

Purpose

Gateway compatible destination vectors containing StrepIII/HA/UltralD

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Type

Empty backbone

Tags

StrepIII-HA-UltraID

Expression

Bacterial and Mammalian

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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MAC_C_RON

Plasmid

#187783

Purpose

MAC-tagged gene expression of human RON

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

RON

Expression

Mammalian

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pECFP18aaEYFP

Plasmid

#109330

Purpose

ECFP-EYFP FRET positive control

Depositor

Gabriele Kaminski Schierle

Article

Kaminski et al Methods Mol Biol. 2014;1076:445-54. doi:

Insert

ECFP-EYFP FRET positive control

Expression

Mammalian

Mutation

ECFP linked to EYFP with 18 aa linker GLRSRAQASNS…

Promoter

CMV

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pLys-M1 backbone

Plasmid

#109386

Purpose

Empty plasmid of the pLys-M1 construct. This is medium copy (p15A), CmR

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

Type

Empty backbone

Expression

Bacterial

Available Since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

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MAC_C_DUX4

Plasmid

#187799

Purpose

MAC-tagged gene expression of human DUX4

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

DUX4 (DUX4 Human)

Expression

Mammalian

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223_EGFR_p.ELREA_746del

Plasmid

#82911

Purpose

Gateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

EGFR (EGFR Human)

Use

Gateway entry vector

Mutation

Deletion of residues 746-750 (ELREA)

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_EGFR_p.769_770insASV

Plasmid

#82880

Purpose

Gateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

EGFR (EGFR Human)

Use

Gateway entry vector

Mutation

ASV insertion between AA 769 and 770

Promoter

None

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR223_EGFR_p.774_775insHV

Plasmid

#82852

Purpose

Gateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

EGFR (EGFR Human)

Use

Gateway entry vector

Mutation

HV insertion between AA 774 and 775

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_EGFR_p.773_774insH

Plasmid

#82855

Purpose

Gateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

EGFR (EGFR Human)

Use

Gateway entry vector

Mutation

Additional H residue inserted between AA 773 and …

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pBABEpuro-ERBB2

Plasmid

#40978

Depositor

Matthew Meyerson

Article

Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

Insert

ERBB2 (ERBB2 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pKP332 (Lenti-OSK1)

Plasmid

#21627

Depositor

Tim Townes

Article

Chang et al Stem Cells. 2009 May . 27(5):1042-9.

Insert

hOCT4 - 2A - hSOX2 - 2A - hKLF4 (POU5F1, SOX2, KLF4 Porcine teschovirus-1, Human)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

Nov. 12, 2009

Availability

Academic Institutions and Nonprofits only

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