We narrowed to 17,055 results for: PAN

Showing: 1841 - 1860 of 17055 results

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pcDNA3.1/Puro-CAG-JEDI-1P-P2A-mCherry-CAAX

Plasmid

#202606

Purpose

Genetically encoded voltage indicator (GEVI) JEDI-1P fused to membrane-anchored mCherry via P2A, expressed under strong mammalian promoter (CAG)

Depositor

Francois St-Pierre

Article

Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

Insert

JEDI-1P-P2A-mCherry-CAAX

Expression

Mammalian

Promoter

CAG

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO5.U6.DR130(CasRX)_Non-targeting sgRNA.EFS.tRFP657

Plasmid

#212962

Purpose

Lentiviral plasmid for the measurement of RfxCas13d (CasRx) nuclease activity. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.

Depositor

Roland Rad

Article

Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s

Insert

DR1_CasRx_non-targeting spacer

Use

CRISPR and Lentiviral

Tags

TagRFP657

Expression

Mammalian

Promoter

U6

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO5.U6.DR130(CasRX)_GFP-targeting sgRNA.EFS.tRFP657

Plasmid

#212963

Purpose

Lentiviral plasmid for the measurement of RfxCas13d (CasRx) nuclease activity. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.

Depositor

Roland Rad

Article

Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s

Insert

DR1_CasRx_GFP targeting spacer

Use

CRISPR and Lentiviral

Tags

TagRFP657

Expression

Mammalian

Promoter

U6

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-DIO-GRAB_ATP1.0

Plasmid

#167581

Purpose

Express the ATP sensor GRAB_ATP1.0 in Cre positive cells

Depositor

Yulong Li

Article

Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

Insert

ATP sensor GRAB_ATP1.0 (P2RY1 Human)

Use

AAV

Promoter

EFS

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pAM5188

Plasmid

#120087

Purpose

Cyanobacteria cloning vector based on Synechococcus elongatus PCC 7942 small plasmid, harboring a toxin-antitoxin cassette to improve the plasmid stability without antibiotic selection.

Depositor

Susan Golden

Article

Chen et al Microbiology. 2016 Dec;162(12):2029-2041

Insert

pAM4788 where the promoter/reporter device (PconII-LTRBS-yemGFP) was replaced with a MCS from the pBluescript vector (pBS MCS).

Use

Other

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-GfaABC1D-GRAB_ATP1.0mut

Plasmid

#167580

Purpose

Express the ATP-insensitive control sensor GRAB_ATP1.0mut in astrocytes

Depositor

Yulong Li

Article

Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

Insert

ATP-insensitive sensor GRAB_ATP1.0mut (control sensor) (P2RY1 Human)

Use

AAV

Promoter

GfaABC1D

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-JEDI-1P-Kv-WPRE

Plasmid

#202608

Purpose

Double floxed soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector expressed under the mammalian promoter (EF1a)

Depositor

Francois St-Pierre

Article

Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

Insert

JEDI-1P-Kv

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1a

Available Since

June 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-GRAB_ATP1.0mut

Plasmid

#167578

Purpose

Express the ATP-insensitive control sensor GRAB_ATP1.0mut in neurons

Depositor

Yulong Li

Article

Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

Insert

ATP-insensitive sensor GRAB_ATP1.0mut (control sensor) (P2RY1 Human)

Use

AAV

Promoter

hSyn

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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hKv2.1-HA

Plasmid

#111525

Purpose

Expresses human WT KCNB1 in mammalian cells

Depositor

Federico Sesti

Article

Cotella et al J Neurosci. 2012 Mar 21;32(12):4133-44.

Insert

human Kv2.1 + HA (KCNB1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pAcCas12nHS_RV6_PT1_empty

Plasmid

#203810

Purpose

Plasmid containing a CMV-driven NLS-AcCas12n-PT1-NLS(human-codonoptimized)cassette and a U6-driven sgRNA_V6 cassette(containing BsaI sites for spacer insertion). Carbenicillin resistance.

Depositor

Quanjiang Ji

Article

Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

Insert

AcCas12nHS_RV6_PT1

Use

CRISPR

Promoter

CMV

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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NgOE FUNgGW

Plasmid

#92231

Purpose

Lentiviral expression of human Ng

Depositor

Weifeng Xu

Article

Hwang et al Biol Psychiatry. 2021 Feb 1;89(3):256-26

Insert

Ng (NRGN Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

hUbC

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-FRT-JEDI-1P-Kv-WPRE

Plasmid

#202617

Purpose

Genetically encoded voltage indicator (GEVI) JEDI-1P flanked by FRT in AAV production vector expressed under the mammalian promoter (EF1a), FLP recombinase-dependent

Depositor

Francois St-Pierre

Article

Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

Insert

FRT-JEDI-1P-Kv

Use

AAV; Flp/frt

Expression

Mammalian

Promoter

EF1a

Available Since

June 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCA15-Ubc-NLS- scFV-tdTomato

Plasmid

#199446

Purpose

Expression of scFV-tdTomato that binds to the GCN4 peptide from the SunTag System in mammalian cells

Depositor

Baohui Chen

Article

Xu et al J Virol. 2022 Dec 21;96(24):e0092022. do

Insert

scFV-tdTomato

Use

Lentiviral

Expression

Mammalian

Promoter

human ubiquitin C (UBC)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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Neurod1-Atoh7HA3

Plasmid

#44438

Depositor

William Klein

Article

Mao et al Development. 2013 Feb;140(3):541-51. doi

Insert

Atoh7-3xHA knock-in (Atoh7 Mouse)

Use

Cre/Lox and Mouse Targeting

Tags

Neurod1 left arm, Neurod1 right arm, and loxC2-Re…

Expression

Mammalian

Available Since

July 23, 2013

Availability

Academic Institutions and Nonprofits only

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pYPQ239A (dFnCas12a)-TV

Plasmid

#136379

Purpose

CRISPRa Gateway entry clone for catalytically dead FnCas12a fused with TV activation system

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

dFnCas12a-TV

Use

CRISPR

Expression

Plant

Mutation

D917A

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ141-ZmUbi-RZ-Aa3.8.4

Plasmid

#136375

Purpose

Gateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 3.8 with MS2 at the third stem loop is used

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ292 (AaCas12b)-D570A-TV

Plasmid

#136380

Purpose

CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

AaCas12b (D570A)-TV

Use

CRISPR

Expression

Plant

Mutation

D570A

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ290-D570A

Plasmid

#129673

Purpose

Gateway entry clone for CRISPR-Cas12b systems

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

AacCas12b

Use

CRISPR; Gateway compatible aaccas12b-d570a entry …

Expression

Plant

Mutation

D570A; AacCas12b is rice codon optimized

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ292-D570A-SRDX

Plasmid

#129684

Purpose

Gateway entry clone for CRISPR-Cas12b systems

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

AaCas12b-SRDX

Use

CRISPR; Gateway compatible aacas12b entry clone

Expression

Plant

Mutation

D570A; AaCas12b is rice codon optimized

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ290-D570A-SRDX

Plasmid

#129682

Purpose

Gateway entry clone for CRISPR-Cas12b systems

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

AacCas12b-SRDX

Use

CRISPR; Gateway compatible aaccas12b entry clone

Expression

Plant

Mutation

D570A; AacCas12b is rice codon optimized

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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