We narrowed to 5,083 results for: mcs

1. EGFP-hPMCA2z/b

   Plasmid

   #47584

   Depositor

   Emanuel Strehler

   Article

   Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

   Insert

   pMCA2z/b (ATP2B2 Human)
   

   Tags

   EGFP-HA-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

2. AV4-EF1-NMNAT2

   Plasmid

   #124970

   Purpose

   Express NMNAT2

   Depositor

   Kazuhiro Oka

   Article

   Ljungberg et al Hum Mol Genet. 2012 Jan 15;21(2):251-67.

   Insert

   NMNAT2, GFP (NMNAT2 Human)
   

   Use

   AAV

   Promoter

   EF1-alpha

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAS4.1w-tet-on-NORAD

   Plasmid

   #125010

   Purpose

   express in mammalian cell

   Depositor

   Ruey-Hwa Chen

   Article

   Tan et al Oncogene. 2019 Jul;38(28):5612-5626. doi

   Insert

   Homo sapiens non-coding RNA activated by DNA damage (NORAD), long non-coding RNA (NORAD Human)
   

   Expression

   Mammalian

   Promoter

   TRE-Tight

   Available Since

   April 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Bov.hsc70.RSET.FL.wt(NarI)

   Plasmid

   #12532

   Depositor

   David McKay

   Article

   Ha et al J Biol Chem. 1997 Oct 31. 272(44):27796-

   Insert

   bovine 70 kDa heat shock cognate protein full length wild type
   

   Expression

   Bacterial

   Mutation

   wild type

   Available Since

   Sept. 1, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNP1 tdTomato toehold switch

   Plasmid

   #107354

   Purpose

   Expresses tdTomato (a DsRed derivative) in Ecoli off a T7 promoter when pNP1 tdTomato trigger is co-expressed

   Depositor

   James Collins

   Article

   Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

   Insert

   toehold switch 5 + tdTomato
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDH-EF1a-tetR-T2A-iRFP

   Plasmid

   #176028

   Purpose

   construct expresses tet repressor (tetR) to reduce leakiness together with the fluorochrome iRFP720

   Depositor

   Irmela Jeremias

   Article

   Liu et al Biomark Res. 2020 Sep 16;8:46. doi: 10.1

   Inserts

   tetR
   iRFP
   

   Use

   Lentiviral

   Available Since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. G1397 DddAtox-C–dSpCas9

   Plasmid

   #157836

   Purpose

   expresses split DddAtox-Cas9 construct in mammalian cells

   Depositor

   David Liu

   Article

   Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

   Insert

   bpNLS–G1397 DddAtox-C–dSpCas9–UGI–UGI–bpNLS
   

   Use

   CRISPR

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV5-ratERK2-Q103A

   Plasmid

   #40816

   Depositor

   Natalie Ahn

   Article

   Emrick et al Proc Natl Acad Sci U S A. 2006 Nov 28;10

   Insert

   MAPK1 (Mapk1 Rat)
   

   Tags

   His6

   Expression

   Mammalian

   Mutation

   Q103A

   Promoter

   CMV

   Available Since

   Oct. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pCMV Gab1(PRD)-mCherry-HOTag3

   Plasmid

   #190026

   Purpose

   Transiently expresses CluMPS for reporting on EML4-ALK oncogene clusters in mammalian cells.

   Depositor

   Lukasz Bugaj

   Article

   Mumford et al Cell Syst. 2024 Feb 21;15(2):166-179.e7.

   Insert

   Gab1(PRD) CluMPS
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. Pu2-3::tag::sl1(G16C)

    Plasmid

    #79002

    Purpose

    Expression of exogenous SL1 in multiple tissues of C. elegans

    Depositor

    Ge Zhan, Xiao Liu

    Article

    Ma et al Nucleic Acids Res. 2016 Aug 23. pii: gkw

    Insert

    sls-1.2 (sls-1.2 Nematode)
    

    Tags

    illumina-adapter

    Expression

    Worm

    Mutation

    G16C

    Promoter

    F08G2.17

    Available Since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNP1 Aqua toehold switch

    Plasmid

    #107356

    Purpose

    Expresses Aquamarine (an eCFP derivative) in Ecoli off a T7 promoter when pNP1 Aqua trigger is co-expressed

    Depositor

    James Collins

    Article

    Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

    Insert

    toehold switch 8 + Aquamarine
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJL1-PD-4xDQNAT

    Plasmid

    #128391

    Purpose

    In vitro expression of H. influenzae protein D with C terminal 4xDQNAT glycosylation sites

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    Protein D
    

    Tags

    4xDQNAT glycosylation tag and 6xHis tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. lentiCRISPR-v2-FE-PuroR

    Plasmid

    #186746

    Purpose

    Modified lentiCRISPRv2 3rd generation lentiviral backbone (Addgene Cat# 52961) containing an FE hairpin

    Depositor

    Richard Sherwood

    Article

    Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. Dynamin I-K44A-non-tagged

    Plasmid

    #32695

    Depositor

    Robert Lefkowitz

    Article

    Ahn et al J Biol Chem. 2002 Jul 19;277(29):26642-5

    Insert

    Dynamin I (Dnm1 Rat)
    

    Expression

    Mammalian

    Mutation

    K44A

    Promoter

    CMV

    Available Since

    Nov. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pX459-HypaCas9-TUBA1B_sgRNA

    Plasmid

    #183889

    Purpose

    pX459V2.0-HypaCas9 plasmid with TUBA1B sgRNA for N-terminal tagging of alpha-tubulin in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    TUBA1B sgRNA spacer (TUBA1B Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX459-HypaCas9-mR2-TUBA1B_sgRNA

    Plasmid

    #183888

    Purpose

    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and TUBA1B sgRNA for N-terminal tagging of alpha-tubulin in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    TUBA1B sgRNA spacer (TUBA1B Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. HSC1-D4Z4-GiP

    Plasmid

    #58542

    Purpose

    Derived from HSC1-GiP where the D4Z4 insulator is cloned upstream of the EF1 alpha promoter

    Depositor

    James Ellis

    Article

    Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1

    Inserts

    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    D4Z4 repeat, partial
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    Human EF1-alpha

    Available Since

    Sept. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUC19-TSV (linearized in VP2)

    Plasmid

    #70032

    Purpose

    pUC19-TSV (linearized in VP2) contains a genome of the Trichodysplasia spinulosa-associated polyomaviruse (GenBank NC_014361) linearized and cloned in the VP2-coding region

    Depositor

    Mariet Feltkamp

    Article

    van der Meijden et al PLoS Pathog. 2010 Jul 29;6(7):e1001024.

    Insert

    TSV full genome, linearized in VP2 gene
    

    Use

    Cloning vector

    Expression

    Bacterial

    Available Since

    Dec. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. 782 pMEActMEK

    Plasmid

    #31166

    Depositor

    Nathan Lawson

    Article

    Covassin et al Dev Biol. 2009 May 15. 329(2):212-26.

    Insert

    MEK (MAP2K1 Human)
    

    Use

    Entry vector

    Mutation

    Ser218Glu Ser222Asp; activated MEKK

    Available Since

    Aug. 11, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pJL1-EPA-DNNNS-DQNRT

    Plasmid

    #128397

    Purpose

    In vitro expression of P. aeruginosa exotoxin A with DNNNS and DQNRT internal glycosylation sites

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    Exotoxin A
    

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    242DNNNS246 and 384DQNRT388 engineered glycosylat…

    Promoter

    T7

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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