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We narrowed to 6,562 results for: nls

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Showing: 1841 - 1860 of 6562 results

  1. pCMV-PE2-P2A-hMLH1dn

    Plasmid
    #174827
    Purpose
    Mammalian expression of SpCas9 prime editor 2 with P2A-human MLH1dn (codon optimized)
    Depositor
    David Liu
    Publication
    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PE2-P2A-hMLH1dn
    Tags
    SV40 bpNLS
    Expression
    Mammalian
    Mutation
    Detailed in manuscript; deletion of residues 754-…
    Promoter
    CMV
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    174827-plasmid-map-sequence-id-343635

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  4. pcDNA3-mCherry-TSC2-NLS

    Plasmid
    #192441
    Purpose
    Encodes mCherry-tagged wild-type TSC2 isoform 5 targeted to the nucleus.
    Depositor
    Jin Zhang
    Publication
    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry-TSC2-NLS (TSC2 Human)
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    192441-plasmid-map-sequence-id-379077

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  8. pHeEf1a-mEGFP-NLS

    Plasmid
    #205012
    Purpose
    The vector contains 1kbp of the upstream and downstream regions of the ef1a gene from Hypsibius exemplaris, with the mEGFP gene with NLS sequence positioned in between.
    Depositor
    Kazuharu Arakawa
    Publication
    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mEGFP-NLS flanked by 1kbp upstream and downstream of the ef1a gene from Hypsibius exemplaris
    Use
    Tardigrade expression
    Available since
    Aug. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    205012-plasmid-map-sequence-id-405256

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  9. PiggyBac-TDP-43deltaNLS-V5-APEX

    Plasmid
    #229081
    Purpose
    Doxycycline-inducible expression of NLS-mutated TDP-43 fused to V5-tag and APEX for proximity labeling, containing BFP in the backbone
    Depositor
    Eran Hornstein
    Insert
    TDP-43deltaNLS (TARDBP Human)
    Tags
    APEX, BFP, and V5
    Expression
    Mammalian
    Mutation
    K82A, R83A, K84A, K95A, K97A and R98A for mutatio…
    Promoter
    TRE3G
    Available since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    229081-plasmid-map-sequence-id-457517

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  11. plenti-As-Cas12a-2xNLS

    Plasmid
    #155047
    Purpose
    Lentiviral vector expressing human codon-optimized _C-terminal Myc-tagged _Acidaminococcus sp. BV3L6 (As)-Cas12a nuclease with N- and C-terminal NLS and Neomycin/G418/Geneticin resistance from EF-1alpha promoter
    Depositor
    Benjamin Blencowe, Jason Moffat
    Publication
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    AsCas12a
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS, Myc-tag and SV40 NLS
    Expression
    Mammalian
    Promoter
    EF-1a
    Available since
    Aug. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    155047-plasmid-map-sequence-id-308054

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  15. mApple-PCNA-19-NLS-4

    Plasmid
    #54937
    Purpose
    Localization: Nucleus, Excitation: 568, Emission: 592
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PCNA-19 NLS (PCNA Human)
    Tags
    mApple
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article

  16. LN-entry+NLS

    Plasmid
    #128011
    Purpose
    Vector containing the lambdaN-entry expression cassette with SV40 NLS and a separate expression cassette driving mCherry via the traffic jam enhancer
    Depositor
    Julius Brennecke
    Publication
    Batki et al Nat Struct Mol Biol. 2019 Aug;26(8):720-
    Insert
    LN-3xFlag-SV40NLS
    Use
    Lamdan entry vector with nls to generate tetherin…
    Tags
    LN-3xFlag-SV40NLS
    Available since
    Aug. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    128011-plasmid-map-sequence-id-251573

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  17. pMT-myl7-NLS-BirA-2A-mCherry_Ras

    Plasmid
    #80061
    Purpose
    Myl7 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Publication
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Zebrafish biotagging
    Tags
    3x HA
    Expression
    Bacterial
    Promoter
    Myl7 promoter
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80061-plasmid-map-sequence-id-152595

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  18. pET-28b-T7-hAsCas12a-enRVR(E174R/S542R/K548V/N552R)-NLS(nucleoplasmin)-6xHis (AAS1931)

    Plasmid
    #114075
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a-enRVR(E174R/S542R/K548V/N552R) with C-terminal NLS and His-tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized AsCas12a-enRVR (E174R/S542R/K548V/N552R) with C-terminal NLS and His-tag
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    E174R, S542R, K548V and N552R
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    114075-plasmid-map-sequence-id-220517

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  20. pEJS581-pCSDest2-AcrIIC4Hpa-FLAG-NLS

    Plasmid
    #113436
    Purpose
    Expresses a FLAG/NLS tagged type II-C anti-CRISPR protein from H. parainfluenzae in mammalian cells
    Depositor
    Erik Sontheimer
    Publication
    Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    type II-C anti-CRISPR
    Use
    CRISPR
    Tags
    FLAG/NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    113436-plasmid-map-sequence-id-225078

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Showing: 1841 - 1860 of 6562 results