175,750 results
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Plasmid#113644PurposePlasmid contains the aminoacyl-tRNA synthetase and tRNA genes from methanococcus jannaschii for charging tyrosine at amber stop codons.DepositorInsertMjYRS and tRNA gene
UseSynthetic BiologyAvailable SinceJuly 27, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
MLRV3:HRE-P53-MAPK/JNK-FOXO-TGFβ
Plasmid#178320PurposeMultiplex luciferase reporter vector, with luciferase reporters for the pathways HRE, P53, AP-1, FOXO and SMAD.DepositorInsertsHRE RedFirefly reporter
P53 FLuc reporter
AP-1 Renilla reporter
FOXO NLuc Reporter
SMAD GrRenilla reporter
UseLuciferase and Synthetic BiologyExpressionMammalianAvailable SinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
VE-cad KI gRNA1
Plasmid#92310PurposeCRISPR-GFP-gRNA for cutting VEcadDepositorAvailable SinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
NylCA-TS_pET28a
Plasmid#215419PurposeExpression construct for NylCA-TS gene, codon optimized for expression in E. coliDepositorInsertNylCA-TS
ExpressionBacterialMutationS111G/A137L/E263QAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
piggyBAC-H2B mCherry-XL (R125K)
Plasmid#189926PurposeExpresses nuclear-localized mCherry-XL (R125K) in mammalian cellsDepositorInsertmCherry-XL (R125K)
TagsH2BExpressionMammalianMutationdiffers from mCherry by R125K, W143S, I161V, Q163…PromoterCMV7Available SinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAS
Plasmid#60847PurposeExpresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome editing in yeastDepositorInsertsS. pyogenese Cas9
RNA pol III promoter (tRNA-Tyr)
hepatitis delta virus ribozyme, genomic
sgRNA
UseCRISPR and Synthetic BiologyTagsNLS/His8 and TTT 3' extension prior to sgRNAExpressionBacterial and YeastMutationL4 is UUCG tetraloop and guide targets LYP1 (CATA…Available SinceJan. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pME-Sun1-2xsfGFP-6xMYC-pA (JDW 681)
Plasmid#224489PurposeGateway compatible middle entry clone containing Sun1 reporter fusion to 2 copies of sfGFP and 6 MYC tags (Nuclear envelope reporter and affinity tag)DepositorInsertSun1-2xsfGFP-6xMYC-pA
UseGateway CloningAvailable SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR CD22-Megf10 CAR
Plasmid#113016Purposefor production of lentivirus encoding CD22 CAR-P Megf10DepositorInsertCD22 CAR-P Megf10 intracellular domain
UseLentiviralTagsGFPExpressionMammalianAvailable SinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETCON-HtsptLB12v.3
Plasmid#45121DepositorInsertHtsptLB12v.3
TagsAga2p for yeast surface display and c-myc epitope…ExpressionYeastPromoterGALAvailable SinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pQE80_T5_P9-ehaA_LacI
Plasmid#186317PurposePlasmid for inducible E coli expression of the P9 helixCAM.DepositorInsertP9-ehaA
ExpressionBacterialAvailable SinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only