Addgene

We narrowed to 26,789 results for: CAL

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pN-iRS3GG

Plasmid

#98858

Purpose

Expresses pheS cassette (in place of an antisense RNA probe, for golden gate cloning) in E. coli (kanR).

Depositor

Lydia Contreras

Article

Vazquez-Anderson et al Nucleic Acids Res. 2017 May 19;45(9):552

Insert

PheS cassette to be selectively replaced by unique asRNA probes

Use

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FLEX-Mac-GFP

Plasmid

#58803

Purpose

AAV-mediated expression of Mac-GFP under the CAG promoter, in floxed/reversed (Cre-dependent) manner

Depositor

Edward Boyden

Article

Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

Insert

Mac-GFP

Use

AAV and Cre/Lox

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pLViP-LifeAct-TagRFPT

Plasmid

#229700

Purpose

Lentiviral expression of LifeAct-TagRFPT in mammalian cells

Depositor

Alpha Yap

Article

Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

Insert

Synthetic construct LifeAct-mTurquoise gene

Use

Lentiviral

Tags

TagRFPT

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDONR223-TAF5-∆ex8

Plasmid

#209048

Purpose

Gateway-compatible entry vector, with insert of the TAF5 isoform lacking the alternative exon-8 (∆ex8)

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

TAF5 deltaexon-8

Use

Gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pLCHKOv3-mClover3

Plasmid

#209034

Purpose

Lentiviral backbone for expressing U6 driven hybrid guide (hg)RNAs with BveI cloning sites, mClover3 and puromycin selection marker

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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Qt-FLAG-TGA-rt9us-antiGFP

Plasmid

#206954

Purpose

Mammalian expression of Qt EMcapsulins targeting GFP with stoichiometry control (~8% surface decoration)

Depositor

Gil Westmeyer

Article

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

QtEnc, antiGFP-intrabody

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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Qt-FLAG-TAA-rt20s-antiGFP

Plasmid

#206955

Purpose

Mammalian expression of Qt EMcapsulins targeting GFP with stoichiometry control (~6% surface decoration)

Depositor

Gil Westmeyer

Article

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

QtEnc, antiGFP-intrabody

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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1M-Qt-FLAG-TGA-rt20s-antiGFP

Plasmid

#206953

Purpose

Mammalian expression of 1M-Qt EMcapsulins targeting GFP with stoichiometry control (~20% surface decoration)

Depositor

Gil Westmeyer

Article

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

MmMT3, QtEnc, antiGFP-intrabody

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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PRPF4-1

Plasmid

#156158

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

PRPF4 (PRPF4 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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SFRS10

Plasmid

#156007

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

SFRS10 (TRA2B Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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RBM8A

Plasmid

#155910

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

RBM8A (RBM8A Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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SFRS2IP

Plasmid

#156012

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

SFRS2IP (SCAF11 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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SFRS17A

Plasmid

#156010

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

SFRS17A (SFRS17A Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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TTR C10S E63C

Plasmid

#190009

Purpose

Expression of human TTR in E coli

Depositor

Peter Wright

Article

Sun et al J Biol Chem. 2022 Jun 18;298(8):102162.

Insert

TTR (TTR Human)

Use

Tags

Expression

Bacterial

Mutation

C10S, E63C

Promoter

T7

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pPodA11

Plasmid

#162843

Purpose

Expression protein designed PodA10 from a TEV cleavable IPTG inducible plasmid with ampicillin resistance

Depositor

Dianne Newman

Article

VanDrisse et al Proc Natl Acad Sci U S A. 2021 Mar 23;11

Insert

Protein Designed Pyocyanin demethylase

Use

Tags

TEV-cleavable 6x-His tag

Expression

Bacterial

Mutation

A53N, I73T, A87V, M99V, A129T from WT PodA

Promoter

T7

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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p3E-SERCA1

Plasmid

#109552

Purpose

Multisite gateway vector for 3' tagging with sarcoplasmic/endoplasmic calcium ATPase 1

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

sarcoplasmic/endoplasmic calcium ATPase 1 (atp2a1 Zebrafish)

Use

Zebrafish plasmids

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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dre-cdk2 3'UTR sicheck2

Plasmid

#121123

Purpose

luciferase assay

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

dre-cdk2 3'UTR (cdk2 Zebrafish)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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CLK1

Plasmid

#155458

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

CLK1 (CLK1 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GCaMP 6m-p2A-ChRmine-Kv2.1-WPRE

Plasmid

#131004

Purpose

bicistronic AAV vector to express GCaMP6m and soma-targeted ChRmine under the control of human Synapsin promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Has Service

AAV8

Insert

ChRmine

Use

AAV

Tags

Kv2.1-HA

Expression

Mutation

Promoter

hSyn

Available since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-FLEX-TeLC-P2A-dTomato

Plasmid

#159102

Purpose

Cre-dependent tetanus toxin light chain construct with nuclear-localized dTomato fluorophore for conditional silencing of neurons.

Depositor

Sandeep Datta

Article

Pashkovski et al Nature. 2020 Jul;583(7815):253-258. doi:

Insert

TeLC-P2A-NLS-dTomato

Use

AAV

Tags

Expression

Mutation

Promoter

hSynapsin

Available since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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