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GTT-ir

Plasmid

#81112

Purpose

Gene Tagging vector TRP/iRFP - Plasmid for tandem infrared RFP (tdiRFP) labeling of endogenous proteins with a TRP selection marker, originating from pSIVt (ID:81092)

Depositor

Serge Pelet

Article

Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

Insert

tdiRFP

Use

Cre/Lox

Tags

tdiRFP

Expression

Yeast

Available Since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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GTL-r

Plasmid

#81195

Purpose

Gene Tagging vector LEU/RFP - Plasmid for monomeric Cherry (mCherry) labeling of endogenous proteins with a LEU selection marker, originating from pSIVl (ID:81090)

Depositor

Serge Pelet

Article

Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

Insert

mCherry

Use

Cre/Lox

Tags

mCherry

Expression

Yeast

Available Since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pXN22-Dest

Plasmid

#105085

Purpose

Yeast mating-based Split Ubiquitin Assay, prey vector for Gateway cloning

Depositor

Christopher Grefen

Article

Grefen et al Curr Protoc Neurosci. 2007 Oct;Chapter 5

Type

Empty backbone

Tags

NubG-3xHA

Expression

Yeast

Available Since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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STARR-seq luciferase validation vector_ORI_neg.cont

Plasmid

#99315

Purpose

Negative control for luciferase validation vectors

Depositor

Alexander Stark

Article

Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

Insert

neutral region; chr3: 55198740-55200041

Use

Luciferase; Starr-seq luciferase validation vector

Expression

Mammalian

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pINDUCER-21-RUNX1-P2A-ERG

Plasmid

#97045

Purpose

Expresses RUNX1-P2A-ERG in mammalian cells. *Please see notes below on P2A cleaving efficiency.

Depositor

George Daley

Article

Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

Insert

Runt Related Transcription Factor 1, ETS-Related Gene

Use

Lentiviral

Expression

Mammalian

Promoter

Tet on

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-Rheb (Q64L)-pcw107

Plasmid

#64607

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

RHEB (RHEB Human)

Use

Lentiviral

Tags

FLAG

Mutation

Q64L

Promoter

PGK

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
pGEX-RhoA

Plasmid

#12202

Depositor

Jonathan Chernoff

Article

Bagrodia et al J Biol Chem. 1995 Sep 29. 270(39):22731-

Insert

RhoA (RHOA Human)

Tags

GST

Expression

Bacterial

Available Since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

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pDONR223_STAT3_WT

Plasmid

#82235

Purpose

Gateway Donor vector containing STAT3, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

STAT3 (STAT3 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pGG-9.47_VP1,3 #2515

Plasmid

#74237

Purpose

Modified plasmid with a different backbone that yields higher of AAV-AS vectors. Encodes VP1 and VP3 of AAV9.47

Depositor

Miguel Sena-Esteves

Article

Choudhury et al Mol Ther. 2015 Dec 28. doi: 10.1038/mt.2

Inserts

AAV2 Rep
AAV9.47 VP1
AAV9.47 VP3

Use

AAV

Available Since

April 6, 2016

Availability

Academic Institutions and Nonprofits only

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Teto Ngn2

Plasmid

#62223

Purpose

Inducible expression of Ngn2

Depositor

Kristin Baldwin

Article

Blanchard et al Nat Neurosci. 2015 Jan;18(1):25-35. doi:

Insert

Neurog2 (Neurog2 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

March 5, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-fDIO-GFP-IRES-tTA

Plasmid

#118026

Purpose

AAV vector for sparse and bright labeling in a cell-type specific manner

Depositor

Minmin Luo

Article

Lin et al Nat Methods. 2018 Nov 19. pii: 10.1038/s

Insert

TRE-fDIO-GFP-IRES-tTA

Use

AAV

Mutation

See depositor comments below

Promoter

TRE

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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U6-sgRNA(MS2)_EF1a-MS2-P65-HSF1

Plasmid

#92120

Purpose

Expression plasmid for both MS2-P65-HSF1 activator helper complex and sgRNA with two MS2 loops at tetraloop and stemloop 2 contains BsaI sites for insertion of spacer sequences.

Depositor

Ervin Welker

Article

Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

Insert

MS2-P65-HSF1 (HSF1 Synthetic, Human)

Use

CRISPR

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-BAZ2A

Plasmid

#65373

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

BAZ2A (BAZ2A Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-TagRFP657

Plasmid

#96939

Purpose

Retroviral plasmid containing TagRFP657 for labeling of cells

Depositor

Lee Grimes

Article

Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

Insert

Tag Red Fluorescent Protein 657

Expression

Mammalian

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1_SEPT2

Plasmid

#71547

Purpose

mammalian expression of Sept 2 with a GFP fusion

Depositor

Elias Spiliotis

Article

Bowen et al J Cell Biol. 2011 Jul 25;194(2):187-97.

Insert

Septin 2 (Septin2 Mouse)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2016

Availability

Academic Institutions and Nonprofits only

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pRRL MND GFP

Plasmid

#36247

Depositor

David Rawlings

Article

Astrakhan et al Blood. 2012 Mar 19.

Insert

GFP (eGFP )

Use

Lentiviral

Promoter

MND

Available Since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA4-Beclin1 243-450

Plasmid

#24392

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

Insert

Beclin1 (BECN1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains amino acids 243-450.

Available Since

April 1, 2010

Availability

Academic Institutions and Nonprofits only
6xHis-SNAP-PI(4)P Probe

Plasmid

#211509

Purpose

For expression of recombinant biosensor for detection of Phosphatidylinositol-4-Phosphate (PI(4)P) from bacteria

Depositor

Hannes Maib, David Murray

Article

Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

Insert

SidC (P4C)

Tags

6xhis-SNAP

Expression

Bacterial

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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6xHis-SNAP-PI(3)P Probe

Plasmid

#211508

Purpose

For expression of recombinant biosensor for detection of Phosphatidylinositol-3-Phosphate (PI(3)P) from bacteria

Depositor

Hannes Maib, David Murray

Article

Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

Insert

FYVE-Hrs x 2 (PIKFYVE Human)

Tags

6xhis-SNAP

Expression

Bacterial

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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NM1-5S-tRNA-SgH

Plasmid

#128178

Purpose

Expresses gRNA using fusion of R. toruloides 5S rRNA and tRNA-Arg as promoter

Depositor

Huimin Zhao

Article

Schultz et al Biotechnol Bioeng. 2019 Apr 30. doi: 10.

Insert

gRNA cloning cassette

Use

CRISPR

Expression

Yeast

Promoter

5S rRNA-tRNA(Arg)

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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