We narrowed to 3,815 results for: LYC;

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LENTICRISPR-HNRNPD_sgRNA1

Plasmid

#201598

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

HNRNPD (HNRNPD Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-PABPC1_sgRNA1

Plasmid

#201608

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

PABPC1 (PABPC1 Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pFBP-2_6m.mutant

Plasmid

#162842

Purpose

Yeast expression of the mutant #2_6m for the RNA-based sensor of fructose-1,6-bisphosphate #2_6

Depositor

Matthias Heinemann

Article

Ortega et al Cell Chem Biol. 2021 Apr 22. pii: S2451-

Insert

2_6m_RNA-device

Expression

Yeast

Available Since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Δ18 B.1.351v2/wtRBD

Plasmid

#169467

Purpose

Encodes SARS-CoV-2 B.1.351v2/wtRBD Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

Insert

SARS-CoV-2 B.1.351v2/wtRBD Spike truncated to remove 18 amino acids (S )

Expression

Mammalian

Available Since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNAFut9

Plasmid

#161021

Purpose

a fucosyltransferase 9 (Fut9) expression plasmid, containing full-length mouse Fut9 cloned into pcDNA3.1+

Depositor

Andreas Faissner

Article

Hennen et al J Biol Chem. 2011 May 6;286(18):16321-31

Insert

Fucosyltransferase 9 (Fut9 Mouse)

Expression

Mammalian

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pFB1_hMSH2_G674A

Plasmid

#134874

Purpose

Expression human MSH2 G674 mutant protein using baculovirus

Depositor

Peggy Hsieh

Article

Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

Insert

human MSH2_G674A (MSH2 Human)

Expression

Insect

Mutation

Changed Glycine to Alanine at aa#674

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223 LC3B2 G120A

Plasmid

#123076

Purpose

Gateway entry clone encoding human MAP1LC3B2 G120A

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3 beta 2 (MAP1LC3B2 Human)

Use

Gateway Cloning

Mutation

Glycine 120 to Alanine

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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gh267

Plasmid

#106866

Purpose

expression of gRNA targeting ALG13

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

ALG13 (ALG13 Human)

Expression

Mammalian

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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gh173

Plasmid

#106834

Purpose

expression of gRNA targeting UGGT2

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

UGGT2 (UGGT2 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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gh139

Plasmid

#106809

Purpose

expression of gRNA targeting EXTL3

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

EXTL3 (EXTL3 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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gh68

Plasmid

#106739

Purpose

expression of gRNA targeting B3GNT2

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

B3GNT2 (B3GNT2 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pMDC43-SEIPIN2

Plasmid

#96977

Purpose

Express GFP-tagged Arabidopsis SEIPIN2 gene in plants.

Depositor

Kent Chapman

Article

Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

Insert

SEIPIN2 (AT1G29760 Mustard Weed)

Tags

GFP

Expression

Plant

Promoter

35S

Available Since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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pGEXndoE(E662Q)

Plasmid

#47716

Purpose

Expresses GST-EndoE(E662Q) from E. faecalis in E. coli

Depositor

Mattias Collin, Vincent Fischetti

Article

Collin et al J Biol Chem. 2004 May 21;279(21):22558-7

Insert

ndoE

Tags

GST-EndoE(E186Q) and Glutathione S-transferase

Expression

Bacterial

Mutation

Changed Glu-662 to Gln

Promoter

tac

Available Since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

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pSC-hTDG-IntE2

Plasmid

#52270

Purpose

Bacterial expression of human TDG C-terminally fused to GyrA intein, SUMO-E2 and GST

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Insert

hTDG (TDG Human)

Tags

GyrA-intein-Ubc9-GST

Expression

Bacterial

Promoter

T7

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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p413GPD-hTPI Lys13Arg

Plasmid

#50722

Purpose

expression of the human TPI Lys13Arg allele in yeast

Depositor

Markus Ralser

Article

Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

Insert

Triosephosphate isomerase 1 (TPI1 Human)

Expression

Yeast

Mutation

changed Lysine 13 to Argenine

Promoter

GPD

Available Since

Feb. 20, 2014

Availability

Academic Institutions and Nonprofits only

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p413GPD-hTPI Ile170Thr

Plasmid

#50721

Purpose

expression of the human TPI Ile170Thr allele in yeast

Depositor

Markus Ralser

Article

Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

Insert

Triosephosphate isomerase 1 (TPI1 Human)

Expression

Yeast

Mutation

Isoleucine 170 to Threonine

Promoter

GPD

Available Since

Feb. 20, 2014

Availability

Academic Institutions and Nonprofits only

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p413GPD-hTPI Ile170Val

Plasmid

#50720

Purpose

expression of the human TPI Ile170Val allele in yeast

Depositor

Markus Ralser

Article

Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

Insert

Triosephosphate isomerase 1 (TPI1 Human)

Expression

Yeast

Mutation

Isoleucine 170 to Valine

Promoter

GPD

Available Since

Feb. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pET20b- hTPI Ile170Val

Plasmid

#50724

Purpose

overexpression of the human TPI1 Ile170Val allele in E.coli

Depositor

Markus Ralser

Article

Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

Insert

Triosephosphate isomerase 1 (TPI1 Human)

Tags

6xHIS tag

Expression

Bacterial

Mutation

changed Isoleucine 170 to Valine

Promoter

T7

Available Since

Jan. 31, 2014

Availability

Academic Institutions and Nonprofits only

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Vh-HA-2xDHFR

Plasmid

#20218

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

2xDHFR (folA E. coli)

Tags

HA and Vh

Expression

Mammalian

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
pCINTBeta1

Plasmid

#14067

Depositor

Richard Hynes

Article

Tamkun et al Cell. 1986 Jul 18. 46(2):271-82.

Insert

Chicken Beta 1 Integrin (ITGB1 Chicken)

Expression

Mammalian

Mutation

PstI-BglI fragment was blunt end cloned and EcoRI…

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only