We narrowed to 3,526 results for: mpo

1. pAAV-TRE-FLEX-eNpHR-EYFP

   Plasmid

   #89876

   Purpose

   To generate AAV virus that will express eNpHR-EYFP in Cre-dependent recombination under control of tetO promoter

   Depositor

   Hyungbae Kwon

   Article

   Lee et al Nat Methods. 2017 May;14(5):495-503. doi

   Insert

   eNpHR=EYFP
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Promoter

   TetO

   Available Since

   May 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAL204

   Plasmid

   #22282

   Purpose

   Expresses PhyB-mCFP with prenylation sequence from Kras, for red-light-induced recruitment of PIF to the plasma membrane

   Depositor

   Christopher Voigt

   Article

   Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

   Insert

   pCMV-PhyB(1-908)-10aaLinker-mCFP-Kras4BCT (PHYB Mustard Weed)
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Available Since

   Feb. 19, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn-DIO-GRAB_eCB2.0

   Plasmid

   #164606

   Purpose

   Express the endocannabinoid sensor GRAB_eCB2.0 in Cre positive neurons

   Depositor

   Yulong Li

   Article

   Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

   Insert

   Endocannabinoid sensor GRAB_eCB2.0
   

   Use

   AAV

   Mutation

   S383T

   Promoter

   hSyn

   Available Since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-FAS-GCaMP6f-WPRE

   Plasmid

   #216277

   Purpose

   AAV vector with hSynapsin promoter and LoxFAS sites for Cre-Off expression of GCaMP6f (GCaMP6f will not be expressed in neurons that express Cre recombinase)

   Depositor

   Guohong Cui

   Article

   Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do

   Insert

   GCaMP6f
   

   Use

   AAV and Cre/Lox

   Tags

   6XHIS and Xpress

   Promoter

   human synapsin 1 (hSyn)

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Myc-βarrestin2-LOV-Turbo_pCDNA3

   Plasmid

   #199671

   Purpose

   expresses Myc-βarrestin2-LOV-Turbo in mammalian cells

   Depositor

   Alice Ting

   Article

   Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

   Insert

   LOV-Turbo
   

   Tags

   Myc and βarrestin2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pABCb10-GFP

   Plasmid

   #19031

   Depositor

   Orian Shirihai

   Article

   Graf et al J Biol Chem. 2004 Oct 8. 279(41):42954-6

   Insert

   ATP-binding cassette, sub-family B (MDR/TAP), member 10 (Abcb10 Mouse)
   

   Tags

   HIS

   Expression

   Mammalian

   Available Since

   Oct. 1, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. iRFP682-Smad2

   Plasmid

   #118943

   Purpose

   Mammalian expression of human Smad2 protein

   Depositor

   Won Do Heo

   Article

   Li et al ACS Synth Biol. 2018 Feb 16;7(2):443-451

   Insert

   iRFP682-Smad2 (SMAD2 Human)
   

   Tags

   iRFP682

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLHCX-FLAG-mPKM1

   Plasmid

   #44240

   Depositor

   Lewis Cantley, Matthew Vander Heiden

   Article

   Christofk et al Nature. 2008 Mar 13;452(7184):230-3. doi

   Insert

   Pyruvate Kinase M1 (Pkm Mouse)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 10, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pB[vasa-Cas9 PUb-ZsGreen]

   Plasmid

   #169012

   Purpose

   For germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.

   Depositor

   Max Scott

   Article

   Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

   Insert

   Cas9
   

   Use

   Piggybac

   Expression

   Insect

   Promoter

   Drosophila melanogaster vasa

   Available Since

   April 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCRISPR-cBEST-v2-PermE

    Plasmid

    #209446

    Purpose

    improved pCRISPR-cBEST plasmid with optimized sgRNA cloning site

    Depositor

    Tilmann Weber

    Article

    Maleckis et al ACS Chem Biol. 2024 Feb 10. doi: 10.1021

    Insert

    Codon optimized APOBEC1-nCas9-UGI fusion protein
    

    Use

    CRISPR and Synthetic Biology

    Promoter

    PtipA; sgRNA expression from ermE* promoter

    Available Since

    March 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. GEF-H1 FLARE212.pa

    Plasmid

    #134930

    Purpose

    GEF-H1 biosensor that does not have GEF activity.

    Depositor

    Klaus Hahn

    Article

    Azoitei et al J Cell Biol. 2019 Sep 2;218(9):3077-3097

    Insert

    GEF-H1(1-212)-yPet-4X linker-Cerulean3-GEF-H1(213-end)
    

    Tags

    None.

    Expression

    Bacterial and Mammalian

    Promoter

    CMV and T7

    Available Since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCR3-FLAG-Ubc13-WT

    Plasmid

    #43938

    Depositor

    Bryant Darnay

    Article

    Lamothe et al J Biol Chem. 2007 Feb 9;282(6):4102-12.

    Insert

    ubiquitin-conjugating enzyme E2N (UBE2N Human)
    

    Tags

    2x FLAG

    Expression

    Mammalian

    Available Since

    Dec. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. PA-Dre

    Plasmid

    #169706

    Purpose

    Photoactivatable Dre recombinase for AAV production and expression in mammalian cells

    Depositor

    Dali Li

    Article

    Li et al Proc Natl Acad Sci U S A. 2020 Dec 29;11

    Insert

    Dre246-nMag-T2A-pMag-Dre247
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CAG

    Available Since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLGB36

    Plasmid

    #135621

    Purpose

    Suicide vector for allelic replacement in Bacteroides species including B. fragilis strain 638R, erythromycin selection and aTC-inducible ss-Bfe3 counterselection

    Depositor

    Laurie Comstock

    Article

    Ito et al mBio. 2020 Feb 4;11(1). pii: mBio.03238-

    Insert

    bfe3
    

    Use

    Bacteroides suicide vector with inducible counter…

    Tags

    signal sequence for periplasmic targeting

    Promoter

    Bacteroides promoter regualted by TetR transcript…

    Available Since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. GEF-H1 FLARE191.pa

    Plasmid

    #134931

    Purpose

    GEF-H1 biosensor that has GEF activity.

    Depositor

    Klaus Hahn

    Article

    Azoitei et al J Cell Biol. 2019 Sep 2;218(9):3077-3097

    Insert

    GEF-H1(1-191)-yPet-4X linker-Cerulean3-GEF-H1(192-end)
    

    Tags

    None.

    Expression

    Bacterial and Mammalian

    Promoter

    CMV and T7

    Available Since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. p415 TEF roGFP2‐Tsa2ΔCPΔCR

    Plasmid

    #83239

    Purpose

    The catalytically inactive genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCPΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For cytosolic expression.

    Depositor

    Tobias Dick

    Article

    Morgan et al Nat Chem Biol. 2016 Jun;12(6):437-43. do

    Insert

    roGFP2-Tsa2dCpdCr
    

    Tags

    roGFP2 fusion with Tsa2dCpdCr

    Expression

    Yeast

    Promoter

    TEF

    Available Since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. ZIKV_Sensor_27B_LacZ

    Plasmid

    #75006

    Purpose

    Toehold switch sensor to detect Zika Virus

    Depositor

    James Collins, Alexander Green

    Article

    Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

    Insert

    ZIKV_sensor_27B_LacZ
    

    Tags

    LacZ

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAL149

    Plasmid

    #22275

    Purpose

    Expresses PhyB-mCherry with prenylation sequence from Kras, for red-light-induced recruitment of PIF to the plasma membrane

    Depositor

    Christopher Voigt

    Article

    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

    Insert

    PhyB(1-908) (PHYB Mustard Weed)
    

    Use

    Synthetic Biology

    Tags

    mCherry-Kras4BCT (prenylation sequence)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. MSCV-mir-17-92

    Plasmid

    #64100

    Purpose

    expresses mir-17-92

    Depositor

    Lin He

    Article

    Olive et al Elife. 2013 Oct 15;2:e00822. doi: 10.755

    Insert

    miR-17-92 (MIR17HG Human)
    

    Expression

    Mammalian

    Promoter

    MSCV

    Available Since

    June 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-hGfap-R-CaMP1.07-WPRE-SV40

    Plasmid

    #164140

    Purpose

    AAV vector to express the red Ca2+ indicator R-CaMP1.07 in astrocytes.

    Depositor

    Hirokazu Hirai, Hajime Hirase

    Article

    Oe et al Nat Commun. 2020 Jan 24;11(1):471. doi:

    Insert

    R-CaMP1.07
    

    Use

    AAV

    Promoter

    hGFAP

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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