We narrowed to 2,375 results for: pcas9

1. pYPQAT14

   Plasmid

   #223386

   Purpose

   T-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for dicot plants; NGG PAM; wide working window; A3A/Y130F-CBE_V01 was driven by ZmUbi1 and sgRNA was by AtU3; BASTA for plant selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   ZmUbi-hA3A-Y130F-SpCas9-D10A-UGI-AtU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. PX459-sgRNA047_Podxl

   Plasmid

   #232932

   Purpose

   Expression vector for a sgRNA against the mouse Podxl locus and SpCas9 with T2A-Puromycin resistance gene.

   Depositor

   Danny Reinberg

   Article

   Lucero et al bioRxiv, March 19, 2025

   Insert

   Cas9-T2A-PuroR (Podxl S. pyogenes)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1 Cas9-P2A-dTomato-PS9

   Plasmid

   #231911

   Purpose

   For packaging spCas9, dTomato mRNA into SARS-CoV-2 virus-like particles (VLPs) with a 'PS9' packaging signal

   Depositor

   Sriram Neelamegham

   Article

   Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

   Insert

   Cas9-P2A-dTomato
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1 Cas9-P2A-dTomato-T20

   Plasmid

   #231912

   Purpose

   For packaging spCas9, dTomato mRNA into SARS-CoV-2 virus-like particles (VLPs) with a 'T20' packaging signal

   Depositor

   Sriram Neelamegham

   Article

   Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

   Insert

   Cas9-P2A-dTomato
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330-PCNT

   Plasmid

   #227284

   Purpose

   Expresses SpCas9 and a sgRNA targeting the N-terminus of PCNT for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting N-terminus of PCNT (PCNT Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX458-PLK4

   Plasmid

   #227310

   Purpose

   Expresses SpCas9 and a sgRNA targeting the C-terminus of PLK4 for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting C-terminus of PLK4 (PLK4 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCPH3.1-GS3

   Plasmid

   #204762

   Purpose

   Expression of Cas9 and human H3.1

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

   Insert

   H3.1 (H3C6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCPH2A-GS3

   Plasmid

   #204753

   Purpose

   Expression of Cas9 and human H2A

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

   Insert

   H2A (H2AC20 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCPH2A.1-GS3

   Plasmid

   #204754

   Purpose

   Expression of Cas9 and human H2A.1

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

   Insert

   H2A.1 (H2AC17 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCPH2A.2-GS3

    Plasmid

    #204755

    Purpose

    Expression of Cas9 and human H2A.2

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

    Insert

    H2A.2 (H2AC18 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCPH2A.J-GS3

    Plasmid

    #204757

    Purpose

    Expression of Cas9 and human H2A.J

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

    Insert

    H2AJ (H2AJ Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. psgRNA-GST-EGFP-GPIBY55

    Plasmid

    #213720

    Purpose

    To express a single-guide RNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPIBY55 under a CMV promoter.

    Depositor

    Qilai Huang

    Article

    Ma et al Front Mol Biosci. 2022 Sep 29;9:1016090.

    Insert

    EGFP
    

    Use

    CRISPR

    Tags

    GST

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. psgRNA-GST-EGFP-GPIDAF

    Plasmid

    #213719

    Purpose

    To express a single-guide RNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPIDAF under a CMV promoter.

    Depositor

    Qilai Huang

    Article

    Ma et al Front Mol Biosci. 2022 Sep 29;9:1016090.

    Insert

    EGFP
    

    Use

    CRISPR

    Tags

    GST

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. psgRNA-GST-EGFP-GPICEAM7

    Plasmid

    #213721

    Purpose

    To express a single-guide RNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPICEAM7 under a CMV promoter.

    Depositor

    Qilai Huang

    Article

    Ma et al Front Mol Biosci. 2022 Sep 29;9:1016090.

    Insert

    EGFP
    

    Use

    CRISPR

    Tags

    GST

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCLV3Cas-LT2

    Plasmid

    #210550

    Purpose

    Binary vector expressing the Lineage Tracing system. Cas9 expression driven by CLV3 promoter. LT1 reporter

    Depositor

    Ortrun Mittelsten Scheid

    Article

    Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

    Insert

    CLV3p::spCas9::PEA3At::U6p::AAVS1gRNA::pHTR5::LT2::mCherrySSM
    

    Use

    Tags

    Expression

    Plant

    Mutation

    1bp insertion after AAVS1 target sequence that di…

    Promoter

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. ABE-RA5.5

    Plasmid

    #208316

    Purpose

    Expresses ABE-RA5.5 in mammalian cells

    Depositor

    Weixin Tang

    Article

    Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    TadA-RA5.5-SpCas9 D10A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106V, …

    Promoter

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. HSF2 KO

    Plasmid

    #200208

    Purpose

    gRNA for HSF2 KO

    Depositor

    Sheila Teves

    Article

    Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

    Insert

    HSF2 KO (Hsf2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available Since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. TBPL1 KO 1

    Plasmid

    #200210

    Purpose

    gRNA for TBPL1 KO

    Depositor

    Sheila Teves

    Article

    Kwan et al Elife. 2023 Mar 30;12:e83810. doi: 10.75

    Insert

    TBP1 KO 1 (Tbpl1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available Since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. TBPL1 KO 2

    Plasmid

    #200211

    Purpose

    gRNA for TBPL1 KO

    Depositor

    Sheila Teves

    Article

    Kwan et al Elife. 2023 Mar 30;12:e83810. doi: 10.75

    Insert

    TBP1 KO 2 (Tbpl1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available Since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTij_sg_mmu_Med6_C_terminal

    Plasmid

    #197869

    Purpose

    CRISPR/Cas9/sgRNA plasmid for cutting Med6 C terminal and building MED6-mEmerald/Halo knock-in mESCs

    Depositor

    James Zhe Liu

    Article

    Dong et al bioRxiv 2022.10.26.513917

    Insert

    Med6 (Med6 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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