We narrowed to 8,839 results for: sgRNA

1. pLenti-Cas9-ZNF746

   Plasmid

   #207822

   Purpose

   Dual expression of Cas9 and sgRNA targeting ZNF746

   Depositor

   Ben Major

   Article

   LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

   Insert

   ZNF746 sgRNA (ZNF746 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Promoter

   EF-1a; U6

   Available Since

   Dec. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLentiCRISPRv1_COX4

   Plasmid

   #177983

   Purpose

   lentiviral vector expressing Cas9 and a sgRNA targeting COX4

   Depositor

   Jessica Spinelli, David Sabatini

   Article

   Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

   Insert

   sgRNA targeting COX4
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   U6

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT7gRNA-ttn.1_C3

   Plasmid

   #140866

   Purpose

   sgRNA synthesis vector for ttn.1_C3 (zebrafish titin .1).

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   zebrafish ttn.1_C3 sgRNA for in vitro transcription (ttn.1 Zebrafish, Synthetic)
   

   Use

   In vitro transcription of sgrnas

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYTK050

   Plasmid

   #65157

   Purpose

   Encodes sgRNA Dropout as a Type 234 part to be used in the Dueber YTK system

   Depositor

   John Dueber

   Article

   Lee et al ACS Synth Biol. 2015 Apr 14.

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQdCas9.luxR(mut)-sggfp

   Plasmid

   #236185

   Purpose

   The plasmid pQdCas9.luxR(mut)-sggfp expresses the dCas9 endonuclease and the sgRNA targeting the GFP gene. Additionally, this plasmid contains a mutation in the luxR gene.

   Depositor

   Radhakrishnan Mahadevan

   Insert

   Quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas9, and sgRNA for GFP gene
   

   Promoter

   Quorum sensing promoter

   Available Since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLSB-NAT

   Plasmid

   #166698

   Purpose

   Combined sgRNA/Cas9 pLSB vector with natMX6 (cloNAT) marker. Golden Gate-ready for cloning custom sgRNA sequences.

   Depositor

   Robin Allshire

   Article

   Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do

   Inserts

   tRNA promoter : sgRNA cassette
   adh15 promoter : Cas9 codon-optimised for S. pombe
   

   Expression

   Yeast

   Promoter

   adh15 promoter and tRNA promoter

   Available Since

   April 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSLQ1651-sgTelomere(F+E)

   Plasmid

   #51024

   Purpose

   Lentiviral vector that contains an optimized S. pyogenes sgRNA targeting human telomeres

   Depositor

   Stanley Qi, Bo Huang

   Article

   Chen et al Cell. 2013 Dec 19;155(7):1479-91. doi: 1

   Insert

   Optimized sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   mouse U6 promoter

   Available Since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLSB-KAN

   Plasmid

   #166700

   Purpose

   Combined sgRNA/Cas9 pLSB vector with kanMX6 (G418) marker. Golden Gate-ready for cloning custom sgRNA sequences.

   Depositor

   Robin Allshire

   Article

   Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do

   Inserts

   tRNA promoter : sgRNA cassette
   adh15 promoter : Cas9 codon-optimised for S. pombe
   

   Expression

   Yeast

   Promoter

   adh15 promoter and tRNA Ser promoter

   Available Since

   April 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLSB-HYG

   Plasmid

   #166699

   Purpose

   Combined sgRNA/Cas9 pLSB vector with hphMX6 (hygromycin) marker. Golden Gate-ready for cloning custom sgRNA sequences.

   Depositor

   Robin Allshire

   Article

   Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do

   Inserts

   tRNA promoter : sgRNA cassette
   adh15 promoter : Cas9 codon-optimised for S. pombe
   

   Expression

   Yeast

   Promoter

   adh15 promoter and tRNA promoter

   Available Since

   April 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. PDG459 V3

    Plasmid

    #226958

    Purpose

    CBh-SpCas9-2A-Puro, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSLQ1661-sgMUC4-E3(F+E)

    Plasmid

    #51025

    Purpose

    Lentiviral vector that contains an optimized S. pyogenes sgRNA targeting the repetitive sequence of human MUC4 exon 3

    Depositor

    Stanley Qi, Bo Huang

    Article

    Chen et al Cell. 2013 Dec 19;155(7):1479-91. doi: 1

    Insert

    optimized sgRNA (MUC4 Human, Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    mouse U6

    Available Since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. SaCas9v3-Puro

    Plasmid

    #178813

    Purpose

    SaCas9 with 2A-Puro, and a cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Cbh (Cas9-2A Puro) and U6 (gRNA)

    Available Since

    Aug. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. PDG458 V3

    Plasmid

    #226959

    Purpose

    CBh-SpCas9-2A-GFP, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIA123

    Plasmid

    #234038

    Purpose

    E. coli-C. difficile shuttle vector for CRISPR editing in C. difficile. Pxyl::Cas9-opt Pgdh::sgRNA-cdr_0985. PstI site to add DNA for homology directed repair. MscI and NotI for replacement of sgRNA.

    Depositor

    Craig Ellermeier

    Article

    Bollinger et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

    Inserts

    Cas9
    sgRNA
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    Pgdh and Pxyl

    Available Since

    March 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pIA33

    Plasmid

    #120803

    Purpose

    E. coli-C. difficile shuttle vector for CRISPR interference (CRISPRi) in C. difficile; sgRNA targets rfp; Pxyl::dCas9-opt Pgdh::sgRNA-rfp

    Depositor

    Craig Ellermeier, David S. Weiss

    Article

    Muh et al J Bacteriol. 2019 Feb 11. pii: JB.00711-

    Inserts

    deactivated nuclease dCas9, codon-optimized for C. difficile
    single guide RNA targeting red fluorescent protein
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    Base-pairing region targets red fluorescent prote…

    Promoter

    Pgdh and Pxyl

    Available Since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSCBE3-NG

    Plasmid

    #218159

    Purpose

    This plasmid harbors the base editor SCBE3-NG along with an sgRNA cloning cassette, facilitating cytosine base editing at targets bearing NG PAM in Streptomyces.

    Depositor

    Yuhui Sun

    Article

    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

    Inserts

    scbe3-NG
    a programmable sgRNA cloning cassette
    

    Use

    CRISPR; Sgrna transcription

    Expression

    Bacterial

    Mutation

    Mutations in the portion of SpCas9 : D10A / L1111…

    Promoter

    rpsL promoter

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. BLADE

    Plasmid

    #134912

    Purpose

    Empty backbone for cloning sgRNA sequence to be used in nanoblades system

    Depositor

    Emiliano Ricci, Philippe Mangeot, Théophile Ohlmann

    Article

    Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Promoter

    U6

    Available Since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. BLADE-182

    Plasmid

    #134914

    Purpose

    sgRNA targeting GFP to be used in nanoblade system

    Depositor

    Emiliano Ricci, Philippe Mangeot, Théophile Ohlmann

    Article

    Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10

    Insert

    GFP
    

    Use

    CRISPR and Synthetic Biology

    Promoter

    U6

    Available Since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJW1839

    Plasmid

    #154325

    Purpose

    sgRNA Destination Vector for SapTrap

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    SapTrap sgRNA (F+E) vector, R07E5.16 U6 promoter
    

    Use

    CRISPR

    Expression

    Worm

    Mutation

    F+E mutations in sgRNA

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX330-mitf

    Plasmid

    #69801

    Purpose

    Used to make mitf knockout porcine cell line

    Depositor

    Jianguo Zhao

    Article

    Wang et al Sci Rep. 2015 Aug 21;5:13348. doi: 10.10

    Inserts

    mitf-sgRNA
    SpCas9
    

    Expression

    Mammalian

    Promoter

    Chicken Beta-Actin and U6

    Available Since

    Dec. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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