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DNA repair library with 1,573 sgRNAs

Pooled Library

#177663

Purpose

CRISPR-interference (CRISPRi) library of 1,573 sgRNAs targeting 476 genes encoding factors involved in DNA repair and associated processes

Depositor

Brittany Adamson

Article

Hussmann et al Cell. 2021 Oct 28;184(22):5653-5669.e25.

Species

Homo sapiens

Use

CRISPR

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only
DNA repair library with 336 sgRNAs

Pooled Library

#177664

Purpose

CRISPR-interference (CRISPRi) library of 366 sgRNAs targeting 118 genes encoding factors involved in DNA repair and associated processes

Depositor

Brittany Adamson

Article

Hussmann et al Cell. 2021 Oct 28;184(22):5653-5669.e25.

Species

Homo sapiens

Use

CRISPR

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only
mCerulean-Paxillin-22

Plasmid

#55385

Purpose

Localization: Focal Adhesions, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Shaner et al J Cell Sci. 2007 Dec 15;120(Pt 24):4247-

Insert

Paxillin

Tags

mCerulean

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2014

Availability

Academic Institutions and Nonprofits only
pKTol2C-EGFP

Plasmid

#85598

Purpose

Tol2 transposon with EGFP driven by minimal-CAGs promoter.

Depositor

Karl Clark, Scott Fahrenkrug

Article

Clark et al BMC Biotechnol. 2007 Jul 17;7:42.

Insert

Tol2 transposon with EGFP

Expression

Mammalian

Promoter

mini-CAG

Available Since

Dec. 15, 2017

Availability

Academic Institutions and Nonprofits only

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35 pCR vegf 121

Plasmid

#13060

Depositor

Nathan Lawson

Article

Lawson et al Dev Cell. 2002 Jul . 3(1):127-36.

Insert

vegf121

Available Since

June 18, 2010

Availability

Academic Institutions and Nonprofits only
PG13-mCherry

Plasmid

#195291

Purpose

mCherry reporter containing 13 copies of the p53-binding consensus sequence

Depositor

Hideyuki Oguro

Article

Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

Insert

mCherry

Expression

Mammalian

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pPTn2P-PTK

Plasmid

#85604

Purpose

Passport transposon with a puromycin-thymidine kinase fusion driven by a PGK promoter.

Depositor

Karl Clark, Scott Fahrenkrug

Article

Clark et al BMC Biotechnol. 2007 Jul 17;7:42.

Insert

Passport transposon with a puromycin-thymidine kinase fusion

Expression

Mammalian

Promoter

PGK

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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BBa_J72214-BBa_J72090

Plasmid

#40782

Depositor

Christopher Anderson

Article

Kittleson et al ACS Synth Biol. 2012 Dec 21;1(12):583-9.

Insert

VioABCDE

Use

Synthetic Biology; Phagemid

Expression

Bacterial

Available Since

Oct. 31, 2012

Availability

Academic Institutions and Nonprofits only

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BBa_J72114-BBa_J72100

Plasmid

#40781

Depositor

Christopher Anderson

Article

Kittleson et al ACS Synth Biol. 2012 Dec 21;1(12):583-9.

Insert

lacZ

Use

Synthetic Biology; Phagemid

Expression

Bacterial

Available Since

Oct. 25, 2012

Availability

Academic Institutions and Nonprofits only

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BBa_J72113-BBa_J72152

Plasmid

#40784

Depositor

Christopher Anderson

Article

Kittleson et al ACS Synth Biol. 2012 Dec 21;1(12):583-9.

Insert

Phagemid + GFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 31, 2012

Availability

Academic Institutions and Nonprofits only

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5LTR-puro-3LTR

Plasmid

#86948

Purpose

PiggyBac transposon

Depositor

Rob Mitra

Article

Qi et al Nucleic Acids Res. 2017 Jan 12. pii: gkw

Insert

PiggyBac transposon

Expression

Mammalian

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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pKTol2P-PTK

Plasmid

#85599

Purpose

Tol2 transposon with a puromycin-thymidine kinase fusion driven by a PGK promoter.

Depositor

Karl Clark, Scott Fahrenkrug

Article

Clark et al BMC Biotechnol. 2007 Jul 17;7:42.

Insert

Tol2 transposon with a puromycin-thymidine kinase fusion

Expression

Mammalian

Promoter

PGK

Available Since

Sept. 16, 2017

Availability

Academic Institutions and Nonprofits only

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mCerulean3-N1

Plasmid

#54730

Purpose

Localization: N1 Cloning Vector, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Type

Empty backbone

Tags

mCerulean3

Expression

Mammalian

Available Since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

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mCerulean3-C1

Plasmid

#54605

Purpose

Localization: C1 Cloning Vector, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Type

Empty backbone

Tags

mCerulean3

Expression

Mammalian

Available Since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

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BBa_J72113-BBa_J72103

Plasmid

#40785

Depositor

Christopher Anderson

Article

Kittleson et al ACS Synth Biol. 2012 Dec 21;1(12):583-9.

Insert

AraC-pBad + coi + cin + repL + pacA

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 31, 2012

Availability

Academic Institutions and Nonprofits only

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pCS2vegf165

Plasmid

#22417

Depositor

Nathan Lawson

Article

Lawson et al Dev Cell. 2002 Jul . 3(1):127-36.

Insert

vegf (vegfaa Zebrafish)

Use

Zebrafish expression

Available Since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only

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BBa_J72110-BBa_J72094

Plasmid

#40778

Depositor

Christopher Anderson

Article

Kittleson et al ACS Synth Biol. 2012 Dec 21;1(12):583-9.

Insert

AraC-pBad + coi

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only

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BBa_J72110-BBa_J72097

Plasmid

#40779

Depositor

Christopher Anderson

Article

Kittleson et al ACS Synth Biol. 2012 Dec 21;1(12):583-9.

Insert

RhaRS-pRhaB + Coi

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only

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pCS2vegf121

Plasmid

#22416

Depositor

Nathan Lawson

Article

Lawson et al Dev Cell. 2002 Jul . 3(1):127-36.

Insert

vegf (vegfaa Zebrafish)

Use

Zebrafish expression

Available Since

Jan. 4, 2010

Availability

Academic Institutions and Nonprofits only

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pJL051

Plasmid

#242609

Purpose

Parental vector for building the dual-sgRNA CRISPRi library. modified mU6-sgRNA library vector

Depositor

Brittany Adamson

Article

Simpson et al bioRxiv [Preprint]. 2023 Aug 20:2023.08.

Insert

sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

modified mouse U6

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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