We narrowed to 6,847 results for: GFP expression plasmids

1. pAAV-Ef1a-fDIO-eGFP-2A-Cre-WPRE

   Plasmid

   #203842

   Purpose

   Flp-dependent Cre and EGFP expression

   Depositor

   Ivo Spiegel

   Article

   Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

   Insert

   Cre
   

   Use

   AAV

   Tags

   EGFP-2A

   Expression

   Mutation

   Promoter

   Available since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. mEGFP-NbALFA

   Plasmid

   #159986

   Purpose

   For the expresson and purification of the EGFP-tagged anti-ALFA tag nanobody (NbALFA)

   Depositor

   Motoyuki Hattori

   Article

   Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

   Insert

   mEGFP-NbALFA
   

   Use

   Tags

   8histidine-HRV3C protease cleavage site

   Expression

   Bacterial

   Mutation

   3GS linker between mEGFP and NbALFA

   Promoter

   Available since

   Oct. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB_TRE_sg61-PDGFR_EF1a_eGFP

   Plasmid

   #186310

   Purpose

   Plasmid for inducible mammalian cell expression of the sg61 helixCAM and constitutive expression of an identifying fluorescent protein (eGFP). Flanked with PiggyBac sites for easy line construction

   Depositor

   George Church

   Article

   Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

   Inserts

   sg61-PDGFR
   eGFP
   

   Use

   Tags

   His, myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB_TRE_Z18-PDGFR_EF1a_eGFP

   Plasmid

   #186304

   Purpose

   Plasmid for inducible mammalian cell expression of the Z18 helixCAM and constitutive expression of an identifying fluorescent protein (eGFP). Flanked with PiggyBac sites for easy line construction.

   Depositor

   George Church

   Article

   Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

   Inserts

   Z18-PDGFR
   eGFP
   

   Use

   Tags

   HA, myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCS2_hMGFP-60A

   Plasmid

   #200393

   Purpose

   A plasmid IVT template containing a humanized Monster Green Fluorescent Protein (hMGFP) CDS followed by a template-encoded poly(A) tail. Linearization at an Esp3i site.

   Depositor

   Xiao Wang

   Article

   Aditham et al ACS Chem Biol. 2022 Dec 16;17(12):3352-3

   Insert

   monster GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-C+F-GFP

   Plasmid

   #209699

   Purpose

   Expresses GFP dependent on the combined activity of CRE- and FLP-recombinases

   Depositor

   Peer Wulff

   Article

   Rangel Guerrero et al Neuron. 2024 Apr 10:S0896-6273(24)00197-

   Insert

   GFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CBA

   Available since

   Jan. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pQdCas12a.luxR(mut)-sggfp(A)

   Plasmid

   #236039

   Purpose

   The plasmid pQdCas12a.luxR(mut)-sggfp(A) expresses the dCas12a endonuclease and the sgRNA (design A) targeting the gfp gene. Additionally, it contains a mutation in the luxR gene.

   Depositor

   Radhakrishnan Mahadevan

   Insert

   quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (B) of gfp gene
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   quorum sensing promoter

   Available since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-PERK(LD)-EGFP

   Plasmid

   #225680

   Purpose

   Used to generate stable cell lines that express PERK(LD)-EGFP. This plasmid serves as a control for pLVX-PERK(LD)-EGFP-HOTag3.

   Depositor

   Likun Wang

   Article

   Xu et al Cell Rep. 2024 Jun 25;43(6):114358. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-Syn-GFP

   Plasmid

   #58867

   Purpose

   AAV expression of GFP under the Syn promoter

   Depositor

   Edward Boyden

   Article

   Boyden et al Nat Neurosci. 2005 Sep . 8(9):1263-8.

   Insert

   GFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Syn

   Available since

   Aug. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRS305-mAID-Nb(VHHGFP4)

    Plasmid

    #198412

    Purpose

    Express mAID-Nb(VHHGFP4) under the control of ADH1 promoter in budding yeast

    Depositor

    Takumi Kamura

    Article

    Ogawa et al PLoS Genet. 2023 Jun 14;19(6):e1010731.

    Insert

    mAID-Nb (VHHGFP4)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1 promoter

    Available since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pALS3-Ma-SUMO-sfGFP-WT

    Plasmid

    #212122

    Purpose

    Fluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses SUMO-sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.

    Depositor

    Ryan Mehl

    Inserts

    SUMO-sfGFP-WT
    M. alvus Pyl-tRNA(6)
    

    Use

    Tags

    His6 and SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    araC and lpp

    Available since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-C1-alpha2-chimaerin

    Plasmid

    #59316

    Purpose

    Expression plasmid for GFP-tagged alpha2-chimaerin (GFP at N-terminus of alpha2-chimaerin)

    Depositor

    Peter Scheiffele

    Article

    Beg et al Neuron. 2007 Sep 6;55(5):768-78.

    Insert

    alpha2-chimaerin
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMSCV(gfp)U6sgRNA5(BbsI)-PGKpuroBFP

    Plasmid

    #102798

    Purpose

    Retrovirus for delivery of one sgRNA (self-targeting) - GFP-targeting control plasmid contains GFP instead of PuroR

    Depositor

    Sarah Teichmann

    Article

    Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

    Inserts

    sgRNA
    EGFP
    

    Use

    CRISPR and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    H231L

    Promoter

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. MTOR-F2108L_CD19-CAR-28z-2A-EGFP_AAV6_Donor

    Plasmid

    #211904

    Purpose

    Vector for AAV6 donor production. Targeted CD19-CAR-28z-2A-EGFP transgene integration to the MTOR locus with the dominant rapamycin resistance MTOR-F2108L mutation via homology-directed repair.

    Depositor

    Yannick Doyon

    Article

    Levesque et al bioRxiv 2023.09.14.557485

    Insert

    CD19-CAR-28z-2A-EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK1

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-eA3A-BE5-(NG)-PX458-EGFP

    Plasmid

    #229687

    Purpose

    CRISPR CBE plasmid (C to T edits): eA3A-BE5-NG engineered to include the sgRNA cloning backbone from PX458 and EGFP. Includes BbsI sites for sgRNA cloning downstream of U6 promoter.

    Depositor

    Deborah Marsh

    Article

    Dickson et al Hum Mol Genet. 2023 Jun 27:ddad105. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. FUW-[EF1α-CD19 4-1BB-eGFP]

    Plasmid

    #218342

    Purpose

    Lentiviral transfer plasmids for mammalian expression CD19 CAR

    Depositor

    Haoyi Wang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    CD19 CAR mammalian expression cassette
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-T7-ABE8e-nSpCas9-VRQR-P2A-EGFP (KAC1309)

    Plasmid

    #185913

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized ABE8e-nSpCas9-VRQR-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337R

    Promoter

    CMV and T7

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-T7-ABE8.20m-nSpCas9-VRQR-P2A-EGFP (KAC1166)

    Plasmid

    #185918

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized ABE8.20m-nSpCas9-VRQR-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337R

    Promoter

    CMV and T7

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pB6-Id1-T2A-sfGFP-iCre-ERT2-FNF-TK

    Plasmid

    #139279

    Purpose

    B6J Id1-T2A-sfGFP-iCre-ERT2 allele targeting vector, low copy number

    Depositor

    Mario Capecchi

    Insert

    Id1 (Id1 Mouse)
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    C-terminal fusion of T2A-sfGFP-iCre-ERT2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBS595 tet-hCMV-EGFPcre

    Plasmid

    #11957

    Depositor

    Brian Sauer

    Article

    Le et al Anal Biochem. 1999 Jun 1. 270(2):334-6.

    Insert

    EGFP-cre (cre bacteriophage P1)
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 28, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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