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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 13,123 results for: 112

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Showing: 1881 - 1900 of 13123 results

  6. pRTC2-puro

    Plasmid
    #190567
    Purpose
    Expresses the full-length human L1 (L1.3) retrotransposon and an engineered neomycin retrotransposition reporter to monitor retrotransposition efficiency in cultured mammalian cells.
    Depositor
    Anthony Furano
    Article
    Cook et al Proc Natl Acad Sci U S A. 2015 Apr 7;112
    Insert
    L1 (L1.3) with reporter cassette
    Expression
    Mammalian
    Promoter
    SV40
    Available Since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    190567-plasmid-map-sequence-id-393296

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  8. p5’UTR-ORF1

    Plasmid
    #190566
    Purpose
    Holding vector containing the contiguous 5’UTR and ORF1 regions of human L1 (L1.3) to facilitate subcloning of ORF1 mutants into the full-length L1 reporter pRTC2-puro (Cook et al., 2015).
    Depositor
    Anthony Furano
    Article
    Cook et al Proc Natl Acad Sci U S A. 2015 Apr 7;112
    Insert
    Contiguous regions of the human L1 (L1.3) 5’UTR and ORF1
    Use
    For dna propagation only
    Expression
    Bacterial
    Promoter
    N/A
    Available Since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    190566-plasmid-map-sequence-id-393237

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  12. pAC-crRNA-Km

    Plasmid
    #158712
    Purpose
    Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells and a non-targeting crRNA
    Depositor
    Yi-Cheng Sun
    Article
    Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1
    Insert
    AcGFP (for crRNA cloning)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    TetO
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    158712-plasmid-map-sequence-id-321925

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  15. mCerulean-hCOL1A1

    Plasmid
    #140112
    Purpose
    Expresses fluorescently-tagged human Type I procollagen α1 chain with mCerulean replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site
    Depositor
    Sergey Leikin
    Insert
    Type I procollagen α1 (COL1A1 Human)
    Tags
    mCerulean
    Expression
    Mammalian
    Mutation
    COL1A1 exons 2-3 replaced by fluorescent tag
    Promoter
    CMV
    Available Since
    May 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    140112-plasmid-map-sequence-id-296778

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  17. pJV53-Cas12a

    Plasmid
    #158706
    Purpose
    CRISPR system used for genome editing in Mycobacterium smegmatis, expresses codon-optimized FnCpf1 and the recombination proteins gp60 and gp61
    Depositor
    Yi-Cheng Sun
    Article
    Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1
    Insert
    FnCpf1
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    TetO
    Available Since
    Jan. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    158706-plasmid-map-sequence-id-320865

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  20. pRT801

    Plasmid
    #68112
    Purpose
    Integration vector derived from Streptomyces phage phiBT1. Apramycin resistance gene, oriT, ColE1 origin of replication.
    Depositor
    Maggie Smith
    Article
    Gregory et al J Bacteriol. 2003 Sep;185(17):5320-3.
    Inserts
    aac3(IV)
    oriT
    attP
    int
    Use
    Streptomyces integrating vector
    Available Since
    July 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    68112-plasmid-map-sequence-id-248995

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Showing: 1881 - 1900 of 13123 results