Addgene

We narrowed to 7,590 results for: Lif;

Showing: 1881 - 1900 of 7590 results

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UAS-4x(tRNA::hiw{sgRNA})

Plasmid

#187884

Purpose

Gal4/UAS sgRNA expression targeting hiw

Depositor

Lukas Neukomm

Article

Llobet Rosell et al Elife. 2022 Dec 7;11:e80245. doi: 10.755

Insert

4 sgRNAs targeting hiw

Use

Tags

Expression

Mutation

Promoter

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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UAS-4x(tRNA::dsarm{sgRNA})

Plasmid

#187885

Purpose

Gal4/UAS sgRNA expression targeting dsarm

Depositor

Lukas Neukomm

Article

Llobet Rosell et al Elife. 2022 Dec 7;11:e80245. doi: 10.755

Insert

4 sgRNAs targeting dSarm

Use

Tags

Expression

Mutation

Promoter

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-EGFP barcode-7-SV40 polyA

Plasmid

#190871

Purpose

For barcoded retrograde labeling

Depositor

Yuejun Chen

Article

Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

Insert

EGFP-barcode7

Use

AAV

Tags

Expression

Mutation

WT

Promoter

Available since

Nov. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-EGFP barcode-8-SV40 polyA

Plasmid

#190872

Purpose

For barcoded retrograde labeling

Depositor

Yuejun Chen

Article

Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

Insert

EGFP-barcode8

Use

AAV

Tags

Expression

Mutation

WT

Promoter

Available since

Nov. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-EGFP barcode-6-SV40 polyA

Plasmid

#190870

Purpose

For barcoded retrograde labeling

Depositor

Yuejun Chen

Article

Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

Insert

EGFP-barcode6

Use

AAV

Tags

Expression

Mutation

WT

Promoter

Available since

Nov. 14, 2022

Availability

Academic Institutions and Nonprofits only

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Arabidopsis thaliana Atm3 pET21a+

Plasmid

#172321

Purpose

Expresses AtAtm3 in E.coli

Depositor

Douglas Rees

Article

Fan et al Elife. 2022 Mar 25;11. pii: 76140. doi:

Insert

Arabidopsis thaliana Atm3 (ABCB25 Mustard Weed)

Use

Tags

6xHis tag

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pET303-ColE1-TR-del1-40

Plasmid

#180236

Purpose

Expresses T and R domains of Colicin E1 with N-terminal 40 residue deletion (residues 41-364)

Depositor

Joanna Slusky

Article

Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:

Insert

Colicin E1

Use

Tags

6x His-tag

Expression

Bacterial

Mutation

Truncation of ColE1 containing T and R domains (r…

Promoter

T7

Available since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pET303-ColE1-T-P110A

Plasmid

#180237

Purpose

Expresses T domain of Colicin E1 (residues 1-190) with proline to alanine mutation at residue 110

Depositor

Joanna Slusky

Article

Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:

Insert

Colicin E1

Use

Tags

6x His-tag

Expression

Bacterial

Mutation

Truncation of ColE1 containing T domain of ColE1 …

Promoter

T7

Available since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pF(UG) hSyn Syt1-A'Q-4xGS-NS34a-FLAG (codopt)

Plasmid

#158771

Purpose

Expression of synaptotagmin 1 variant with self-cleaving C2 domain.

Depositor

Edwin Chapman

Article

Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10

Insert

Synaptotagmin 1 1-NS3/4a (Syt1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Flexible linkers, ADVVCC/QMSY cleavage site

Promoter

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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FAK Fus

Plasmid

#186145

Purpose

For expression of recombinant FAK using baculovirus in SF9 cells

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

PTK2 (PTK2 Mouse)

Use

Tags

His6

Expression

Insect

Mutation

C-terminal linker swapped with FUS IDR

Promoter

polyhedrin promoter

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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NK72

Plasmid

#176608

Purpose

To test macrophage engulfment initiation. Lacks the FcyR chain.

Depositor

Meghan Morrissey

Article

Kern et al eLife. 2021 Jun 3;10. pii: 68311. doi: 1

Insert

CD86 (CD86 Human)

Use

Lentiviral

Tags

HA, SNAP-tag and mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-PGK-SNAP-MDMUT-OCT4

Plasmid

#115692

Purpose

Constitutive expression of an OCT4 (Pou5f1) protein tagged with SNAP-tag and a non-functional mitotic degron (MDMUT) for control

Depositor

David Suter

Article

Friman et al eLife. 2019 Dec 3;8. pii: 50087. doi: 10

Insert

SNAP-MDMUT-OCT4

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pFastBac FAK W266A

Plasmid

#186144

Purpose

For expresseion of recombinant FAK using baccolovirus in SF9 cells

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

PTK2 (PTK2 Mouse)

Use

Tags

His6

Expression

Insect

Mutation

DNA mutation T796G;G797C Amino acid mutation W266A

Promoter

polyhedrin promoter

Available since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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Lck

Plasmid

#186130

Purpose

For expression of recombinant Lck in SF9 cells.

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

Lck (Lck Mouse)

Use

Tags

His6

Expression

Insect

Mutation

A1687T

Promoter

polyhedrin promoter

Available since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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FAK Pyk2

Plasmid

#186146

Purpose

For expresseion of recombinant FAK using baccolovirus in SF9 cells

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

PTK2 (PTK2 Mouse)

Use

Tags

His6

Expression

Insect

Mutation

C-terminal linker swapped with Pyk2 linker

Promoter

polyhedrin promoter

Available since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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tdTomato-β-actin3'UTR2

Plasmid

#183717

Purpose

tdTomato with fragment nucleotides 441-676 of beta-actin 3'UTR inserted in 3'UTR

Depositor

Jeffrey Gerst

Article

Dasgupta et al Elife. 2023 May 30;12:e83584. doi: 10.75

Insert

tdTomato with fragment (nucleotides 441-676) of mouse Actb 3'UTR

Use

Lentiviral

Tags

Expression

Mutation

Promoter

UbiC

Available since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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tdTomato--β-actin CDS2

Plasmid

#183714

Purpose

tdTomato with fragment nucleotides 398-818 of beta-actin coding sequence inserted in 3'UTR

Depositor

Jeffrey Gerst

Article

Dasgupta et al Elife. 2023 May 30;12:e83584. doi: 10.75

Insert

tdTomato with fragment (nucleotides 398-818) of mouse Actb coding dsequence

Use

Lentiviral

Tags

Expression

Mutation

Promoter

UbiC

Available since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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tdTomato-β-actin3'UTR1

Plasmid

#183716

Purpose

tdTomato with fragment nucleotides 1-440 of beta-actin 3'UTR inserted in 3'UTR

Depositor

Jeffrey Gerst

Article

Dasgupta et al Elife. 2023 May 30;12:e83584. doi: 10.75

Insert

tdTomato with fragment (nucleotides 1-440) of mouse Actb 3'UTR

Use

Lentiviral

Tags

Expression

Mutation

Promoter

UbiC

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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tdTomato-β-actinCDS3

Plasmid

#183715

Purpose

tdTomato with fragment nucleotides 801-1127 of beta-actin coding sequence inserted in 3'UTR

Depositor

Jeffrey Gerst

Article

Dasgupta et al Elife. 2023 May 30;12:e83584. doi: 10.75

Insert

tdTomato with fragment (nucleotides 801-1127) of mouse Actb coding dsequence

Use

Lentiviral

Tags

Expression

Mutation

Promoter

UbiC

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pJH3410

Plasmid

#179575

Purpose

Punc-129(DB)::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans B-MN -specific expression of miniSOG-SL2 RFP

Depositor

Mei Zhen

Article

Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

Insert

tomm20::miniSOG (tomm-20 Nematode)

Use

Tags

wCherry

Expression

Worm

Mutation

Promoter

Punc-129

Available since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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