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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,938 results for: emb

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Showing: 1921 - 1940 of 11938 results

  10. Bongwoori

    Plasmid
    #63720
    Purpose
    Consists of the triple mutant voltage sensing domain and the optimized linker between the S4 transmembrane domain and the super ecliptic pHlorin A227D
    Depositor
    Bradley Baker
    Publication
    Piao et al J Neurosci. 2015 Jan 7;35(1):372-85. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Bongwoori
    Tags
    Super ecliptic pHlorin A227D
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  12. pGBW-m4134096

    Plasmid
    #152039
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;S-tag
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    May 20, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152039-plasmid-map-sequence-id-300258

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  14. pAAH5

    Plasmid
    #41829
    Purpose
    Used as a PCR template for tagging yeast proteins with the SNAP tag by homologous recombination and selection for hygromycin resistance.
    Depositor
    Melissa Moore
    Publication
    Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.
    Type
    Empty backbone
    Use
    Pcr template
    Expression
    Bacterial and Yeast
    Promoter
    T7,TEF
    Available since
    Oct. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    41829-plasmid-map-sequence-id-69202

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  16. mNeonGreen-Smad3

    Plasmid
    #172098
    Purpose
    Expresses fluorescently tagged human Smad3 in mammalian cells.
    Depositor
    Lea Goentoro
    Publication
    Frick et al Proc Natl Acad Sci U S A. 2017 Apr 4;114
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Smad 3 (Smad3 Mouse)
    Tags
    mNeonGreen
    Expression
    Mammalian
    Mutation
    C49S mutation in mCerulean
    Promoter
    CMV
    Available since
    Dec. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    172098-plasmid-map-sequence-id-341509

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  18. pMAL-SH3(2)-5R

    Plasmid
    #112089
    Purpose
    Bacterial expression plasmid containing His and MBP tags for 5 repeats of the second SH3 domain from human NCK1.
    Depositor
    Michael Rosen
    Publication
    Li et al Nature. 2012 Mar 7;483(7389):336-40. doi
    Insert
    SH3(2)-5R (NCK1 Human)
    Tags
    His6 and MBP
    Expression
    Bacterial
    Mutation
    construct contains only repeats of the second SH3…
    Promoter
    Lac
    Available since
    Feb. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    112089-plasmid-map-sequence-id-236082

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  19. pDG335

    Plasmid
    #100899
    Purpose
    SpCas9n (D10A Nickase mutant) with a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.
    Depositor
    Paul Thomas
    Publication
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    HA
    Expression
    Mammalian
    Mutation
    D10A mutant converts to Nickase
    Promoter
    CBh and U6
    Available since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100899-plasmid-map-sequence-id-195324

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  20. pTUB1:YFP-AID-3HA, DHFR-TS:HXGPRT

    Plasmid
    #87260
    Purpose
    YFP-AID-3HA fusion driven by a minimal TUB1 promoter with an HXGPRT drug selectable marker. The AID CDS is Arabidopsis thaliana auxin-responsive protein IAA17.
    Depositor
    David Sibley
    Publication
    Brown et al MBio. 2017 May 2;8(3). pii: e00375-17. d
    Inserts
    YFP-AID-3HA
    HXGPRT
    Use
    Toxoplasma expression
    Tags
    3HA and AID
    Promoter
    TgDHFR-TS 463 bp and TgTUB1 378 bp
    Available since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    87260-plasmid-map-sequence-id-176554

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Showing: 1921 - 1940 of 11938 results