We narrowed to 6,247 results for: nls

1. pCMV-T7-SpCas9-BPNLS(SV40)-3xFLAG-P2A-mScarlet (MNW001)

   Plasmid

   #174091

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a C-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-mScarlet
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-mScarlet

   Expression

   Mammalian

   Promoter

   pCMV and T7

   Available Since

   Sept. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSEM415 - mosTI unc-119 Pmex-5 - gfp(nls) - tbb-2

   Plasmid

   #182358

   Purpose

   Positive control for mosTI(unc-119). unc-119 rescue and germline nuclear GFP expression after mosTI insertion.

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   GFP
   

   Expression

   Worm

   Promoter

   Pmex-5

   Available Since

   May 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-hSyn-DIO-3xHA-hM3D(Gq)-P2A-ArgiNLS-oScarlet

   Plasmid

   #220608

   Purpose

   Neuron-specific, Cre-dependent co-expression of 3x HA-tagged excitatory hM3D(Gq) DREADD receptor, and a single-cell discriminating version of oScarlet as a reporter.

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   3x HA-hM3D(Gq)-P2A-AgiNLS-oScarlet
   

   Use

   AAV and Cre/Lox

   Tags

   3x HA; ArgiNLS

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAR010 Lenti pEF-dEnAsCas12a-NLS-3XHA-T2A-BSD

   Plasmid

   #195543

   Purpose

   dCas12a effector fusion for manipulating transcription

   Depositor

   Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   Lenti pEF-dEnAsCas12a-NLS-3XHA-T2A-BSD
   

   Use

   CRISPR and Lentiviral

   Tags

   3XHA

   Expression

   Mammalian

   Promoter

   pEF1a

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSEM416 - mosTI unc-119 P_UPN - gfp(nls) - tbb-2

   Plasmid

   #182359

   Purpose

   Positive control for mosTI(unc-119). unc-119 rescue and pan-neuronal nuclear GFP expression after mosTI insertion.

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   Ultra-pan-neuronal positive control pUNP::gfp(NLS)
   

   Expression

   Worm

   Promoter

   Promoter Ultra Pan Neuronal

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCAG-hAsCas12a-RR(S542R/K607R)-NLS(nucleoplasmin)-3xHA (AAS1081)

   Plasmid

   #114094

   Purpose

   CAG promoter expression plasmid for human codon optimized AsCas12a-RR(S542R/K607R) nuclease with C-terminal NLS and HA tag

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized AsCas12a-RR (S542R/K607R)
   

   Tags

   NLS(nucleoplasmin) and 3xHA

   Expression

   Mammalian

   Mutation

   S542R and K607R

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCFJ1985 - [1-2] ENTR - Fluor - TagBFP2(PATC, NLS(2), no_atg, no_stop)

   Plasmid

   #159846

   Purpose

   Three-fragment Gateway compatible flourophore for expression in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   [1-2] ENTR - Fluor - TagBFP2(PATC, NLS(2), no_atg, no_stop)
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. JG1202: CAG-human dLbCpf1(D832A)-NLS-3xHA-P65

   Plasmid

   #104566

   Purpose

   Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to p65 activation domain

   Depositor

   Keith Joung

   Article

   Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

   Insert

   dLbCpf1(D832A)-p65
   

   Tags

   3x HA and NLS

   Expression

   Mammalian

   Mutation

   D832A

   Promoter

   CAG

   Available Since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PL752-OTX2-LA-p2a-EGFPnls-hGHpA-PGK-puroR-OTX2-RA

   Plasmid

   #191690

   Purpose

   For knocking p2a-EGFP into OTX2 locus

   Depositor

   Yuejun Chen

   Article

   Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

   Insert

   OTX2 homology arms and p2a-EGFP
   

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSEM387 - mosTI unc-119 - NNN - gfp - nls - tbb-2

    Plasmid

    #182347

    Purpose

    GFP transcriptional reporter cloning vector for mosTI(unc-119). Transgene inserted upstream of gfp(2xNLS) and tbb-2 3' UTR. MCS or Golden Gate (BsaI: tgcc - MCS - aaaa)

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Type

    Empty backbone

    Expression

    Worm

    Available Since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCAG-hAsCas12a(E174R/S542R)-NLS(nucleoplasmin)-3xHA (AAS826)

    Plasmid

    #114091

    Purpose

    CAG promoter expression plasmid for human codon optimized AsCas12a(E174R/S542R) nuclease with C-terminal NLS and HA tag

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized AsCas12a (E174R/S542R)
    

    Tags

    NLS(nucleoplasmin) and 3xHA

    Expression

    Mammalian

    Mutation

    E174R and S542R

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-CAG-C-intein-C-spC9-H840A-N863A-2xNLS-hGH

    Plasmid

    #112211

    Purpose

    Targeted DNA methylation

    Depositor

    Xingxu Huang

    Article

    Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

    Insert

    C-terminus of dCas9
    

    Use

    AAV

    Mutation

    D10A

    Available Since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSEM389 - mosTI unc-119 - NNN - mScarlet(nls) - tbb-2

    Plasmid

    #182349

    Purpose

    mScarlet transcriptional reporter cloning vector for mosTI(unc-119). Transgene inserted upstream of mScarlet(2xNLS) and tbb-2 3' UTR. MCS or Golden Gate (BsaI: tgcc - MCS - aaaa)

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Type

    Empty backbone

    Expression

    Worm

    Available Since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSEM234 - pEXP[Pmlc-1 | mCherry(2xNLS) | cbr-tbb-2 UTR]

    Plasmid

    #159795

    Purpose

    Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

    Insert

    pEXP[Pmlc-1 | mCherry(2xNLS) | cbr-tbb-2 UTR]
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPB-p53RE(CDKN1A)-CMVmin-mScarlet-I-NLSx3-AU1-PGKpuro

    Plasmid

    #241845

    Purpose

    Expresses p53 transcription fluorescence reporter in mammalian cells.

    Depositor

    Kazuhiro Aoki

    Article

    Tsuruoka et al Cell Struct Funct. 2025 Jun 12. doi: 10.

    Insert

    CDKN1A promoter (CDKN1A Human)
    

    Tags

    mScarlet-I-3xNLS

    Expression

    Mammalian

    Mutation

    CDKN1A promoter (1015 bp) + CMV minimal promoter …

    Promoter

    n/a

    Available Since

    Oct. 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPBneo-CIBN-lin-p53CT(98-393)-R273H-mNeonGreen-NLSx3

    Plasmid

    #241849

    Purpose

    Expresses a negative control of the localizer of Opto-p53 in mammalian cells.

    Depositor

    Kazuhiro Aoki

    Article

    Tsuruoka et al Cell Struct Funct. 2025 Jun 12. doi: 10.

    Insert

    p53 (TP53 Human)
    

    Tags

    CIBN and mNeonGreen

    Expression

    Mammalian

    Mutation

    p53 C-terminus (97-393 aa) containing R273H mutat…

    Promoter

    CAGGS promoter

    Available Since

    Sept. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPBneo-CIBN-lin-p53CT(98-393)-6KQ-mNeonGreen-NLSx3

    Plasmid

    #241848

    Purpose

    Expresses an improved localizer of Opto-p53 in mammalian cells.

    Depositor

    Kazuhiro Aoki

    Article

    Tsuruoka et al Cell Struct Funct. 2025 Jun 12. doi: 10.

    Insert

    p53 (TP53 Human)
    

    Tags

    CIBN and mNeonGreen

    Expression

    Mammalian

    Mutation

    p53 C-terminus (97-393 aa) containing six acetyla…

    Promoter

    CAGGS promoter

    Available Since

    Sept. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPBbsr2-NLSx3-miRFP703-dSal-p53NT(1-97)-5SD-lin-hCRY2

    Plasmid

    #241846

    Purpose

    Expresses an improved actuator of Opto-p53 in mammalian cells.

    Depositor

    Kazuhiro Aoki

    Article

    Tsuruoka et al Cell Struct Funct. 2025 Jun 12. doi: 10.

    Insert

    p53 (TP53 Human)
    

    Tags

    CRY2 and miRFP703

    Expression

    Mammalian

    Mutation

    N-terminus (1-97 aa) containing five phosphorylat…

    Promoter

    CAGGS promoter

    Available Since

    Sept. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCE059-SiT-ddCas12a(E993A)-1xNLS-[Activ]-triplex-DR-M13-DR-pA

    Plasmid

    #202042

    Purpose

    Encodes a DNase-dead AsCpf1 fused to a NLP nuclear localization signal (NLS) and [Activ] transactivation domain. 3' UTR has a MALAT1 triplex, dCas12a direct repeats (DR), and a crRNA cloning site.

    Depositor

    Darren Cusanovich

    Article

    Kang et al ACS Synth Biol. 2025 May 16;14(5):1732-1

    Insert

    ddCas12a(E993A)-1xNLS-[Activ]
    

    Expression

    Mammalian

    Available Since

    Aug. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCE059-SiT-ddCas12a(E993A)-3xNLS-[Activ]-triplex-DR-M13-DR-pA

    Plasmid

    #202043

    Purpose

    Encodes a DNase-dead AsCpf1 fused to a 3x SV40 nuclear localization signal (NLS) and [Activ] transactivation domain. 3' UTR has a MALAT1 triplex, dCas12a direct repeats (DR), and a crRNA cloning site.

    Depositor

    Darren Cusanovich

    Article

    Kang et al ACS Synth Biol. 2025 May 16;14(5):1732-1

    Insert

    ddCas12a(E993A)-3xNLS-[Activ]
    

    Expression

    Mammalian

    Available Since

    Aug. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge