We narrowed to 11,996 results for: pcDNA

1. hKCNQ5(WT).ires.GFP-pcDNA3

   Plasmid

   #204360

   Purpose

   Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

   Depositor

   Aguan Wei

   Article

   Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

   Insert

   KCNQ5 (KCNQ5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/T7 RNApol

   Available since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5FRT-EF-Pdgfralpha-EGFPN

   Plasmid

   #66787

   Purpose

   Expression of murine PDGFRalpha tagged with GFP under the control of EF1a promoter

   Depositor

   Lotte Pedersen

   Article

   Nielsen et al J Cell Sci. 2015 Oct 1;128(19):3543-9. d

   Insert

   PDGFRalpha (Pdgfra Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA4/HisMax-Synectin

   Plasmid

   #35943

   Depositor

   Arie Horowitz

   Article

   Naccache et al Proc Natl Acad Sci U S A. 2006 Aug 22;10

   Insert

   Synectin (Gipc1 Mouse)
   

   Use

   Tags

   6xHIS and Xpress

   Expression

   Mammalian

   Mutation

   K111R

   Promoter

   CMV

   Available since

   June 22, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3-teLuc c-myc

   Plasmid

   #100026

   Purpose

   Enhanced red-shifted bioluminescence when paired with diphenylterazine (DTZ)

   Depositor

   Huiwang Ai

   Article

   Yeh et al Nat Methods. 2017 Sep 4. doi: 10.1038/nm

   Insert

   teLuc, a NanoLuc mutant
   

   Use

   Tags

   c-myc

   Expression

   Mammalian

   Mutation

   D19S, D85N, C164H

   Promoter

   CMV

   Available since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5/FRT/TO_LAMP1_HaloTag9

   Plasmid

   #175541

   Purpose

   Lyzosomal expression of HaloTag9 in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   LAMP1-HaloTag9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Promoter

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA4-STRAD alpha

   Plasmid

   #14889

   Depositor

   Lewis Cantley

   Article

   Shaw et al Proc Natl Acad Sci U S A 2004 Mar 9;101(

   Insert

   STRAD alpha (STRADA Human)
   

   Use

   Tags

   6xHis/Xpress

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 27, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3.1-CHRNA7-mGFP

   Plasmid

   #62629

   Purpose

   mammalian expression of human Alpha7 (CHRNA7) with mEGFP fused into M3-M4 loop

   Depositor

   Henry Lester

   Article

   Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

   Insert

   hAlpha7 (CHRNA7 Human)
   

   Use

   Tags

   mEGFP inserted in M3-M4 loop

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA5/FRT/TO_HaloTag7_LamB1

   Plasmid

   #175528

   Purpose

   Expression of HaloTag7 at the nuclear lamina of the mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   HaloTag7-LamB1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1(-)-qmTQ2-cAMP

   Plasmid

   #225250

   Purpose

   mammalian expression of qmTQ2-cAMP, a cyan fluorescent FLIM biosensor for detecting cAMP

   Depositor

   Yasushi Okada

   Article

   Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

   Insert

   qmTQ2-cAMP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1-GGamma11-GFP2

    Plasmid

    #166780

    Purpose

    Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GGamma11-GFP2 (GNG11 Synthetic, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains an N-terminal GFP2 and GSAG linker

    Promoter

    CMV

    Available since

    April 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA/Myc TCF4

    Plasmid

    #16512

    Depositor

    Bert Vogelstein

    Article

    Korinek et al Science. 1997 Mar 21. 275(5307):1784-7.

    Insert

    TCF4 (TCF7L2 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 16, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1-GGamma10-GFP2

    Plasmid

    #166779

    Purpose

    Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GGamma10-GFP2 (GNG10 Synthetic, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains an N-terminal GFP2 and GSAG linker

    Promoter

    CMV

    Available since

    April 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA GFP10-RHOA

    Plasmid

    #133068

    Purpose

    Expresses RHOA GTPase with tripartite split GFP10

    Depositor

    Stéphanie Cabantous

    Article

    Koraichi et al J Cell Sci. 2018 Jan 8;131(1). pii: jcs.

    Insert

    ras homolog family member A (RHOA Human)
    

    Use

    Tags

    GFP10 (split-GFP tripartite)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-Venus-CAAX

    Plasmid

    #87612

    Purpose

    Plasma membrane-targeted Venus (YFP).

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    Venus-CAAX
    

    Use

    Tags

    6xHis, C-terminal targeting sequence from Kras, T…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA RBD-GFP11

    Plasmid

    #133069

    Purpose

    Expresses RBD domain with tripartite split GFP11 (M4)

    Depositor

    Stéphanie Cabantous

    Article

    Koraichi et al J Cell Sci. 2018 Jan 8;131(1). pii: jcs.

    Insert

    RBD (Rhotekin-Rho Binding Domain)
    

    Use

    Tags

    GFP11 (tripartite split-GFP)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3-myc3-CUL2

    Plasmid

    #19892

    Depositor

    Yue Xiong

    Article

    Ohta et al Mol Cell. 1999 Apr . 3(4):535-41.

    Insert

    cullin 2 (CUL2 Human)
    

    Use

    Tags

    myc3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-ExRai-CKAR

    Plasmid

    #118409

    Purpose

    cpGFP-based excitation-ratiometric biosensor for monitoring Protein Kinase C activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    ExRai-CKAR
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mtsCOXVIIIx2-B-gTEMP/pcDNA3

    Plasmid

    #199806

    Purpose

    Fluorescent temperature indicator B-gTEMP fused with mitochondrial targeting sequence

    Depositor

    Takeharu Nagai

    Article

    Lu et al Nano Lett. 2022 Jul 27;22(14):5698-5707.

    Insert

    B-gTEMP
    

    Use

    Tags

    mtsCOXVIIIx2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3-TRAM-CFP

    Plasmid

    #13027

    Depositor

    Doug Golenbock

    Insert

    Toll-like Receptor Adaptor Molecule (TICAM2 Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-ALK6 Q203D

    Plasmid

    #80883

    Purpose

    mammalian expression of ALK6 Q203D

    Depositor

    Aristidis Moustakas

    Article

    Raja et al Oncotarget. 2016 Jan 12;7(2):1120-43. do

    Insert

    ALK6 (Bmpr1b Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Q203D (constitutively active)

    Promoter

    CMV

    Available since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only