We narrowed to 13,568 results for: cas9 genes

1. PX543 SpCas9(del175-307)

   Plasmid

   #58888

   Purpose

   Truncated form of SpCas9 without REC2 domain

   Depositor

   Feng Zhang

   Article

   Nishimasu et al Cell. 2014 Feb 27;156(5):935-49. doi: 10

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   del amino acids 175-307

   Promoter

   Available since

   Sept. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMuLE ENTR CMV-hCas9 R4-R3

   Plasmid

   #62132

   Purpose

   MuLE (Multiple Lentiviral Expression) Entry vector containing CMV promoter and human codon optimized Cas9 module Compatible with MultiSite Gateway cloning

   Depositor

   Ian Frew

   Article

   Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

   Insert

   human codon optimized Cas9
   

   Use

   CRISPR; Mule gateway entry vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. A3H HapI-Cas9n-UGI

   Plasmid

   #119140

   Purpose

   Base editor made from APOBEC3H Haplotype I

   Depositor

   Reuben Harris

   Article

   Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

   Insert

   APOBEC3H Haplotype I-Cas9 nickase-UGI
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   None

   Promoter

   CMV

   Available since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. hRad51(F86E)–Cas9(D10A)

   Plasmid

   #125564

   Purpose

   Expresses the Rad51(F86E)–Cas9(D10A) fusion construct in mammalian cells under CMV promoter

   Depositor

   David Liu

   Article

   Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

   Insert

   Rad51(F86E)–Cas9(D10A) (RAD51 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28b-NmCas9-Flag H588A

   Plasmid

   #109032

   Purpose

   H588A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

   Depositor

   Yan Zhang

   Article

   Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

   Insert

   CRISPR-associated endonuclease Cas9
   

   Use

   Tags

   Flag Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. Icam 2 SaCas9 YAP sgRNA3

   Plasmid

   #99736

   Purpose

   Expresses SaCas9 and a gRNA targeting mouse YAP

   Depositor

   Yu Huang

   Article

   Wang et al Nature. 2016 Dec 7. doi: 10.1038/nature2

   Insert

   SaCas9 gRNA targeting Yap1 (Yap1 Mouse)
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. PX389 Fluviicola taffensis Cas9

   Plasmid

   #68326

   Purpose

   Fluviicola taffensis Cas9

   Depositor

   Feng Zhang

   Insert

   FtCas9
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. PX388 Bacteroides coprophilus Cas9

   Plasmid

   #68325

   Purpose

   Bacteroides coprophilus Cas9

   Depositor

   Feng Zhang

   Insert

   BcCas9
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. PX378 Sutterella wadsworthensis Cas9

   Plasmid

   #68315

   Purpose

   Sutterella wadsworthensis Cas9

   Depositor

   Feng Zhang

   Insert

   SwCas9
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. PX387 Mycoplasma mobile Cas9

    Plasmid

    #68324

    Purpose

    Mycoplasma mobile Cas9

    Depositor

    Feng Zhang

    Insert

    MmCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. PX392 Azospirillum B510 Cas9

    Plasmid

    #68329

    Purpose

    Azospirillum B510 Cas9

    Depositor

    Feng Zhang

    Insert

    AzoCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX330-Flag-HeFSpCas9-H840A

    Plasmid

    #80456

    Purpose

    high fidelity enhanced SpCas9 mammalian expression vector

    Depositor

    Ervin Welker

    Insert

    HeFSpCas9_H840A
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Aug. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-SMVP-Cas9N-U6-gRNA M4

    Plasmid

    #80937

    Purpose

    Expresses Cas9N in mammalian cells; expresses gRNA M4 for Mstn cleavage.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9N
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSpCas9(BB)-2A-puro-hANKRD11 (exon 5-1)

    Plasmid

    #236612

    Purpose

    Knockouts ANKRD11 in human cells

    Depositor

    Tadashi Nakagawa

    Article

    Sashiyama et al iScience 2025 May 19, 112699

    Insert

    ANKRD11 (ANKRD11 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSpCas9(BB)-2A-puro-mAnkrd11 (intron 3)

    Plasmid

    #236608

    Purpose

    Knockouts Ankrd11 in mouse cells

    Depositor

    Tadashi Nakagawa

    Article

    Sashiyama et al iScience 2025 May 19, 112699

    Insert

    Ankrd11 (Ankrd11 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV-TET2BlastR-Cas9-TagBFP

    Plasmid

    #236726

    Purpose

    Dox Inducible Cas-TagBFP expression

    Depositor

    David Bersten

    Insert

    Cas9-TagBFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. dSpyCas9-E566-GS-LOV2-VPR

    Plasmid

    #231576

    Purpose

    Potent light-deactivated CRISPRa

    Depositor

    Dominik Niopek

    Article

    Wolf et al bioRxiv 2024.12.04.626757

    Insert

    dSpyCas9-VPR with an AsLOV2 insertion behind E566
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. CMV_Zdk3-VPR_P2A_dCas9-AsLOVx3-NLSx3

    Plasmid

    #231568

    Purpose

    Expresses components of the LOOMINA optogenetic transcriptional control system.

    Depositor

    Dominik Niopek

    Article

    Muench et al Nucleic Acids Res. 2024 Dec 16:gkae1237.

    Insert

    All-in-one LOOMINA construct
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. AID-C12*-n'Cas9-BE4max

    Plasmid

    #216730

    Purpose

    Expresses a BE4max base editing construct comprised of the hyperactive AID-C12* and n'Cas9 (Cas9 with H840A mutation) in mammalian cells.

    Depositor

    Rahul Kohli

    Article

    Berrios et al Nucleic Acids Res. 2024 Jan 23:gkae024.

    Insert

    AID-C12*-n'Cas9-BE4max (AICDA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-sgAMPKa2-Cas9-GFP

    Plasmid

    #208050

    Purpose

    Lentiviral vector expressing Cas9 and an sgRNA targeting AMPKa2

    Depositor

    Naama Kanarek

    Article

    Maynard et al Sci Adv. 2024 Feb 2;10(5):eadj9479. doi:

    Insert

    sgPRKAA2 (PRKAA2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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