We narrowed to 6,340 results for: ATC

1. pDIRECT_21C

   Plasmid

   #91130

   Purpose

   Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:hpt II

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. LentiCRISPRv2 NT sgRNA3

   Plasmid

   #164929

   Purpose

   Expresses Non-targeting sgRNA3 control in mammalian cells

   Depositor

   Ji Luo

   Article

   Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

   Insert

   Non-targeting sgRNA3 (verified for knockout)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EFS promoter

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAF123

   Plasmid

   #135921

   Purpose

   All-in-one Sleeping Beauty vector for stable transfection of eukaryotic cells with hygromycin restistance. CMV protomor drives SB100X transposase for transposon integration.

   Depositor

   Andrew S. Flies

   Article

   Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab

   Type

   Empty backbone

   Use

   Transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCS2+-FLAG3-drAgo2

   Plasmid

   #221712

   Purpose

   Expression of zebrafish Ago2 with N-terminal 3xFLAG tag in zebrafish embryos.

   Depositor

   David Bartel

   Article

   Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

   Insert

   drAgo2 (ago2 Zebrafish)
   

   Use

   Fish expression

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. VCP F11.4 gRNA

   Plasmid

   #90938

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human VCP

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   VCP (Guide Designation F11.4)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMD19_STARR-seq_plants

   Plasmid

   #117379

   Purpose

   STARR-seq vector with 35s core promoter for transient expression in plant protoplasts

   Depositor

   Xiaoyu Zhang

   Article

   Ricci et al Nat Plants. 2019 Nov 18. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Tags

   GFP derived from pMDC107

   Expression

   Plant

   Mutation

   Promoter

   35s core promoter

   Available since

   Nov. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA-h-mmPtch1-B-HA-TagBFP

   Plasmid

   #120910

   Purpose

   Expresses BFP tagged Ptch1-B

   Depositor

   Philip Beachy

   Article

   Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

   Insert

   Ptch1 (Ptch1 Mouse)
   

   Use

   Tags

   HA and TagBFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 620-643, truncated at 1291

   Promoter

   CMV

   Available since

   Jan. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAT9651-BEAR-GFP

   Plasmid

   #162989

   Purpose

   BEAR target plasmid with split EGFP and disrupted 5' splice site

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   EGFP split with an intron between amino acids 95-96
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-FLEX-NBL10

   Plasmid

   #69971

   Purpose

   Cre-conditionally expresses the anti-GFP Nanobody-Rpl10a fusion

   Depositor

   Jeffrey Friedman

   Article

   Ekstrand et al Cell. 2014 May 22;157(5):1230-42. doi: 1

   Insert

   NBL10
   

   Use

   AAV and Cre/Lox

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX335-EN475

    Plasmid

    #86231

    Purpose

    For transient expression of spCas9-nickase and one sgRNA targeting the mouse CTCF locus. Use together with pX335-EN477.

    Depositor

    Benoit Bruneau

    Article

    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

    Insert

    spCas9-nickase and sgRNA against mouse CTCF STOP Codon
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. CRISPR-StAR1 (Retro)

    Plasmid

    #222687

    Purpose

    CRISPR-screening

    Depositor

    Ulrich Elling

    Article

    Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    PGK without BbsI cutsite

    Promoter

    Available since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. CRISPR-StAR2 (Retro)

    Plasmid

    #222688

    Purpose

    CRISPR-screening

    Depositor

    Ulrich Elling

    Article

    Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    PGK without BbsI cutsite

    Promoter

    Available since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. CRISPR-StAR3 (Retro)

    Plasmid

    #222689

    Purpose

    CRISPR-screening

    Depositor

    Ulrich Elling

    Article

    Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    PGK without BbsI cutsite

    Promoter

    Available since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. LLP773_pLenti-6xHRBKET-sgRNA

    Plasmid

    #211766

    Purpose

    Multiplexed gRNA plasmid targeting six different gene promoters (PACC1-2, EPCAM-2, B2M-4, KL-3, RBM3-1, HINT1-1), with mCherry selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    gRNAs against six different human gene promoters (PACC1-2, EPCAM-2, B2M-4, KL-3, RBM3-1, HINT1-1)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    pCMV and U6

    Available since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. PB-TRE3G-SOX17

    Plasmid

    #104541

    Purpose

    PiggyBac vector encoding doxycycline-inducible human SOX17

    Depositor

    David Vereide

    Article

    Miller et al Stem Cell Reports. 2018 Jan 9;10(1):73-8

    Insert

    SRY-box 17 (SOX17 Human)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSCALPs-HA-NFAT1 (4-460)-GFP

    Plasmid

    #88879

    Purpose

    Lentiviral vector expressing the fusion protein HA-NFAT1 (4-460)-GFP

    Depositor

    Silvia Monticelli

    Article

    Leoni et al Proc Natl Acad Sci U S A. 2017 Feb 6. pi

    Insert

    HA-NFAT1 (4-460)-GFP fusion protein (Nfatc2 Mouse)
    

    Use

    Lentiviral

    Tags

    GFP fusion protein and HA tag

    Expression

    Mammalian

    Mutation

    amino acids 4-460 only; L78P & L287P *see bel…

    Promoter

    SFFV (spleen focus forming virus)

    Available since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pVRa42_4454

    Plasmid

    #49703

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    ECF42_4454
    

    Use

    Synthetic Biology

    Tags

    His6-PreScission

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pT7-lacO

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAT9752-BEAR-mScarlet

    Plasmid

    #162991

    Purpose

    BEAR target plasmid with split mScarlet and disrupted 5' splice site

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    mScarlet split with an intron between amino acids 110-111
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    disrupted 5' splice site

    Promoter

    CMV

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. LentiCRISPRv2 EGFP sgRNA2

    Plasmid

    #164933

    Purpose

    Expresses EGFP sgRNA2 in mammalian cells

    Depositor

    Ji Luo

    Article

    Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

    Insert

    sgRNA2 targeting Enhanced green fluorescent protein (verified for knockout)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS promoter

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28a-HaloTag7SS-DogTag

    Plasmid

    #171775

    Purpose

    Expresses in bacterial cytoplasm HaloTag7 with DogTag inserted between D139 and E140, as well as C61S and C261S in HaloTag7 to block disulfide bond formation

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    HaloTag7SS-DogTag
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    DogTag inserted in HaloTag7 between residues D139…

    Promoter

    T7

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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