We narrowed to 16,479 results for: igh

1. pLIC-SM-Myc

   Plasmid

   #111189

   Purpose

   High affinity Myc tagging of weakly expressed genes (optimized for Toxoplasma gondii)

   Depositor

   Silvia Moreno

   Article

   Hortua Triana et al J Eukaryot Microbiol. 2018 Apr 19. doi:

   Type

   Empty backbone

   Use

   Toxoplasma gondii expression

   Tags

   Spaghetti Monster Myc Tag

   Expression

   Mutation

   Promoter

   Endogenous

   Available since

   Aug. 2, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pUB-MSP1D1

   Plasmid

   #161609

   Purpose

   Bacterial expression of mutated membrane scaffold protein ("ultra-bright" MSP1D1) for Nanodisc formation

   Depositor

   Stephen Sligar

   Article

   McLean et al Anal Biochem. 2020 Oct 15;607:113860. do

   Insert

   UD-MSP1D1
   

   Use

   Tags

   7-His tag

   Expression

   Bacterial

   Mutation

   mutant of MSP1D1 ( Q138W, L181W, Y236W)

   Promoter

   T7

   Available since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBMN HAYFP-LOV24

   Plasmid

   #49570

   Purpose

   regulate protein stability with light

   Depositor

   Thomas Wandless

   Article

   Bonger et al ACS Chem Biol. 2014 Jan 17;9(1):111-5. d

   Insert

   YFP-LOV24
   

   Use

   Retroviral

   Tags

   HA and YFP

   Expression

   Mutation

   delete the last 3 aa from AsLOV2, with I532A muta…

   Promoter

   LTR

   Available since

   Feb. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSFFV_Gal4LOV[SK22]-VP64-IRES-mCherry (pLZA140)

   Plasmid

   #203907

   Purpose

   Encodes LightsOut (AsLOV2(408-543) inserted in Gal4 SK22) transcription factor in mammalian cells

   Depositor

   Jared Toettcher

   Article

   Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

   Insert

   Gal4LOV(SK22)-VP64-IRES-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA FLAG TORC1 (1-634)

   Plasmid

   #25718

   Depositor

   Marc Montminy

   Article

   Conkright et al Mol Cell. 2003 Aug . 12(2):413-23.

   Insert

   TORC1 (1-634) (CRTC1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 28, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGal4AD-CIBN

   Plasmid

   #28246

   Purpose

   Expresses Gal4 Activation Domain fused to CIBN (aa1-170) for blue-light-induced transcription activation

   Depositor

   Chandra Tucker

   Article

   Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

   Insert

   CIB1 (1-171aa)
   

   Use

   Tags

   Gal4AD

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 23, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSUM-Kan-MCS2-gfp

   Plasmid

   #109290

   Purpose

   Expresses high levels of gfp in mycobacteria

   Depositor

   Nicolai van Oers

   Article

   Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

   Insert

   GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   Kanamycin

   Available since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. p-mCherry-N1-ChloC

   Plasmid

   #85461

   Purpose

   Historical plasmid. For inhibition of neuronal activity, #66709 is a light-gated chloride channel with improved anion selectivity (iChloC)

   Depositor

   Peter Hegemann

   Article

   Wietek et al Science. 2014 Mar 27.

   Insert

   ChloC
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   E90R, T159C

   Promoter

   CMV (+enhancer)

   Available since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. Nano-lantern/pRSETB

   Plasmid

   #51969

   Purpose

   bacterial expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging

   Depositor

   Takeharu Nagai

   Article

   Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

   Insert

   Nano-lantern
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   S257G in Rluc8

   Promoter

   Available since

   March 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. LAMP-CeNL_pcDNA3

    Plasmid

    #89514

    Purpose

    Cyan color variant of bright luminescent protein, fused to Lysosomal-associated membrane protein 1

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    CeNL
    

    Use

    Tags

    LAMP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. LAMP-ReNL_pcDNA3

    Plasmid

    #89532

    Purpose

    Red color variant of bright luminescent protein, fused to Lysosomal-associated membrane protein 1

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    ReNL
    

    Use

    Tags

    LAMP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAW-AlphaLobe

    Plasmid

    #113255

    Purpose

    E. coli expression vector for His-MBP-Cas9 Alpha-Helical Lobe

    Depositor

    Jennifer Doudna

    Article

    Wright et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    S. pyogenes Cas9 alpha-helical lobe
    

    Use

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Mutation

    residues 56–714; See paper for description

    Promoter

    T7

    Available since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. GWF058

    Plasmid

    #133609

    Purpose

    UAS-mCherry/CMV-Gal4DBD-NS3a-P2a-DNCR2-VPR

    Depositor

    Dustin Maly

    Article

    Foight et al Nat Biotechnol. 2019 Sep 9. pii: 10.1038

    Insert

    Gal4DBD-NS3a, DNCR2-VPR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDS220

    Plasmid

    #34980

    Purpose

    Expresses a low-affinity ePDZ variant fused to mCherry in yeast. Illumination with 440 nm light induces binding to LOVpep.

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    ePDZb–mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF

    Available since

    March 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSN818

    Plasmid

    #190690

    Purpose

    Mammalian expression plasmid of anti-kinesin (Mouse). Expresses H2 IgG (heavy chain and light chain).

    Depositor

    Shinsuke Niwa

    Article

    Niwa et al Cytoskeleton (Hoboken). 2023 Apr 10. doi

    Insert

    anti-kinesin (Mus musculus) recombinant mouse monoclonal antibody; IgG heavy and light chains
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAL101

    Plasmid

    #22474

    Purpose

    Expresses APB domain of PIF6 fused to mYFP, for red-light-induced binding to Phy

    Depositor

    Christopher Voigt

    Article

    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

    Insert

    PIF6APB
    

    Use

    Synthetic Biology

    Tags

    mYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. GWF297

    Plasmid

    #133612

    Purpose

    TET3G-2xMS2(wt+f6), NLS-MCP-ANR-ANR-P2a-BFP

    Depositor

    Dustin Maly

    Article

    Foight et al Nat Biotechnol. 2019 Sep 9. pii: 10.1038

    Insert

    TET3G-2xMS2(wt+f6), MCP-ANR-ANR, BFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRK010

    Plasmid

    #69874

    Purpose

    expression of Channelrhodopsin-2 XXL (extra high expression and long open state, D156C) mutant

    Depositor

    Robert Kittel, Georg Nagel

    Article

    Dawydow et al Proc Natl Acad Sci U S A. 2014 Sep 23;11

    Insert

    Channelrhodopsin-2
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    D156C (XXL)

    Promoter

    Drosophila Hsp70

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOD_009

    Plasmid

    #159520

    Purpose

    Rifampicin version of the scarless genome engineering pDEL plasmid (Tikh et al. 2016) compatible with Salmonella

    Depositor

    Elisabeth Raleigh

    Article

    Zaworski et al PLoS Genet. 2022 Apr 4;18(4):e1009943. d

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pJBJZ_001

    Plasmid

    #155357

    Purpose

    Gentamycin derivative of lambda-red pKD3 plasmid from Datsenko and Wanner (2000)

    Depositor

    Elisabeth Raleigh

    Article

    Zaworski et al PLoS Genet. 2022 Apr 4;18(4):e1009943. d

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge