We narrowed to 23,606 results for: crispr

1. NM9-SpCas9-NLS3

   Plasmid

   #128177

   Purpose

   Expresses R. toruloides codon-optimized SpCas9 under PGK1 promoter

   Depositor

   Huimin Zhao

   Article

   Schultz et al Biotechnol Bioeng. 2019 Apr 30. doi: 10.

   Insert

   SpCas9-NLS3
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   pPGK1

   Available Since

   April 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. NAX_CAGGS_CAS9_mcherry

   Plasmid

   #167862

   Purpose

   PiggyBac compatible plasmid expressing spCas9

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   Cas9
   

   Use

   CRISPR

   Available Since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. U6.3>gRNA.f+e

   Plasmid

   #99139

   Purpose

   Empty gRNA scaffold with "Flip" and "Extension" modifications with optimized chicken specific U6 promoter

   Depositor

   Marianne Bronner

   Article

   Gandhi et al Dev Biol. 2017 Dec 1;432(1):86-97. doi:

   Insert

   gRNA
   

   Use

   CRISPR

   Promoter

   chicken U6.3

   Available Since

   Aug. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEF_dCas9

   Plasmid

   #68416

   Purpose

   Transient expression of Sp-dCas9 in mammalian cells, under an EF1-alpha promoter.

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D10A/H841A

   Promoter

   human EF1[alpha]

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. p201G Cas9

   Plasmid

   #59178

   Purpose

   Cas9 driven by double 35S, GFP for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

   Depositor

   Wayne Parrott

   Article

   Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

   Inserts

   Cas9
   sGFP
   

   Use

   CRISPR

   Promoter

   2x35S

   Available Since

   Sept. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMCB306

   Plasmid

   #89360

   Purpose

   sgRNA expression vector with GFP, puromycin resistance, BlpI+BstXI cloning sites

   Depositor

   Michael Bassik

   Article

   Han et al Nat Biotechnol. 2017 Mar 20. doi: 10.103

   Insert

   GFP, puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   April 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. U6.3>Control.gRNA.f+e

   Plasmid

   #99140

   Purpose

   Control gRNA under the regulation of an optimized chicken specific U6 promoter

   Depositor

   Marianne Bronner

   Article

   Gandhi et al Dev Biol. 2017 Dec 1;432(1):86-97. doi:

   Insert

   Control gRNA
   

   Use

   CRISPR

   Promoter

   Chicken U6.3

   Available Since

   Aug. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBBK28 pHomL-TDH3p-GFPdropout-Link-Neon(gfp)-SSA1t-HomR (AmpR)

   Plasmid

   #179012

   Purpose

   Parent vector for markerless yeast integration cassettes with C-terminal Neon(gfp)-tagged genes

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   Aug. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. p15A_SpyCas9_CmR

   Plasmid

   #119162

   Purpose

   Expresses S. pyogenes Cas9

   Depositor

   Vincent Noireaux

   Article

   Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

   Insert

   Cas9 from S. pyogenes (NEWENTRY )
   

   Use

   Crispr

   Available Since

   Dec. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJAT30

    Plasmid

    #204294

    Purpose

    For marking CRISPR HDR integrant with DsRed expressed in flight muscles. Second U6 promoter for using two gRNAs.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    MHC-DsRed
    

    Use

    CRISPR

    Promoter

    MHC

    Available Since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTPGI_dCas9_VP64

    Plasmid

    #49013

    Purpose

    encodes yeast-optimized dCas9_VP64 synthetic transcription factor

    Depositor

    Timothy Lu

    Article

    Farzadfard et al ACS Synth Biol. 2013 Sep 11.

    Insert

    dCas9_VP64 (codon-optimized for expression in S. cerevisiae)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    SV40 NLS

    Expression

    Yeast

    Promoter

    pTPGI (galactose+aTc inducible)

    Available Since

    Jan. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV hU6-gRNA(anti-sense) hUbC-VP64-dCas9-VP64-T2A-GFP

    Plasmid

    #66707

    Purpose

    expresses a single gRNA and VP64-dCas9-VP64

    Depositor

    Charles Gersbach

    Inserts

    hU6-gRNA expression cassette
    S.Pyogenes VP64-dCas9-VP64
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    VP64

    Mutation

    D10A and H840A

    Promoter

    hU6 and hUbC

    Available Since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDHt/sk-PC

    Plasmid

    #92125

    Purpose

    Expression of codon-optimized Cas9 with Ppdc promoter in Trichoderma reesei

    Depositor

    Zhihua Zhou

    Article

    Liu et al Cell Discov. 2015 May 12;1:15007. doi: 1

    Insert

    Cas9
    

    Use

    CRISPR; Trichoderma reesei expression

    Promoter

    Ppdc

    Available Since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKHH030

    Plasmid

    #89358

    Purpose

    hU6 sgRNA cloning vector for CDKO system

    Depositor

    Michael Bassik

    Article

    Han et al Nat Biotechnol. 2017 Mar 20. doi: 10.103

    Insert

    GFP, Puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. EC59103

    Plasmid

    #191785

    Purpose

    LbCas12a expression in plants

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

    Insert

    AtUbi10::Cas12a+ 8 introns-T-E9
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    D156R

    Available Since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pV1200

    Plasmid

    #111429

    Purpose

    CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at ENO1 - Recyclable for serial mutagenesis (Sap2-FLP)

    Depositor

    Gerald Fink

    Article

    Vyas et al Sci Adv. 2015;1(3):e1500248.

    Insert

    CaCas9/sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Oct. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCpf1

    Plasmid

    #122185

    Purpose

    Bacterial genome editing plasmid with kanamycin resistance marker

    Depositor

    Cheng-Cai Zhang

    Article

    Niu et al ACS Synth Biol. 2018 Dec 10. doi: 10.102

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. JME4599

    Plasmid

    #129660

    Purpose

    NATex_CrisprCas9-yl_RFP: CAS9 vector with Nourseothricin ex marker for gRNA cloning using GoldenGate

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. NAX_CAGGS_CAS9_Hygro

    Plasmid

    #167859

    Purpose

    PiggyBac compatible plasmid expressing spCas9

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    Cas9
    

    Use

    CRISPR

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJA58

    Plasmid

    #59933

    Purpose

    gRNA for cleavage at dpy-10(cn64) locus in C elegans

    Depositor

    Andrew Fire

    Article

    Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

    Insert

    dpy-10(cn64) gRNA
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Sept. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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