We narrowed to 69 results for: 8453

1. pLKO.1 puro

   Plasmid

   #8453

   Purpose

   3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses puromycin for selection.

   Depositor

   Bob Weinberg

   Article

   Stewart et al RNA 2003 Apr;9(4):493-501.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   July 18, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

These results may also be relevant.

1. pUC118-FLIP-FlpE-Puro

   Plasmid

   #84539

   Purpose

   a mock vector for generating FLIP-FlpE targeting vectors

   Depositor

   Bon-Kyoung Koo

   Article

   Andersson-Rolf et al Nat Methods. 2017 Jan 30. doi: 10.1038/n

   Insert

   FLIP-FlpE - Puro
   

   Use

   Cre/Lox and Mouse Targeting

   Available Since

   Nov. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC118-FLIP-Puro

   Plasmid

   #84538

   Purpose

   a mock vector for generating FLIP targeting vectors

   Depositor

   Bon-Kyoung Koo

   Article

   Andersson-Rolf et al Nat Methods. 2017 Jan 30. doi: 10.1038/n

   Insert

   FLIP - Puro
   

   Use

   Cre/Lox and Mouse Targeting

   Available Since

   Jan. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

3. pAG415-ADH1p-HA-rap1-K9R

   Plasmid

   #84531

   Purpose

   This plasmid can be used to express HA-tagged Rap1-K9R from the ADH promoter in yeast

   Depositor

   Jorrit Enserink

   Article

   Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

   Insert

   RAP1 (RAP1 Budding Yeast)
   

   Tags

   HA

   Expression

   Yeast

   Mutation

   K(43,61,69,117,240,246,527,582,651)R

   Promoter

   ADH1

   Available Since

   Jan. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

4. pAG415-GPDp-EGFP-rap1-K3NR

   Plasmid

   #84533

   Purpose

   This plasmid can be used to express EGFP-tagged Rap1K3NR from the GDP promoter in yeast

   Depositor

   Jorrit Enserink

   Article

   Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

   Insert

   RAP1 (RAP1 Budding Yeast)
   

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   K(43,117,118)R

   Promoter

   GPD

   Available Since

   Dec. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pAG415-GPDp-EGFP-rap1-K3CR

   Plasmid

   #84534

   Purpose

   This plasmid can be used to express EGFP-tagged Rap1K3CR from the GDP promoter in yeast

   Depositor

   Jorrit Enserink

   Article

   Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

   Insert

   RAP1 (RAP1 Budding Yeast)
   

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   K(462,463,651)R

   Promoter

   GPD

   Available Since

   Dec. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pAG415-GPDp-EGFP-rap1-K9R

   Plasmid

   #84535

   Purpose

   This plasmid can be used to express EGFP-tagged Rap1K9R from the GDP promoter in yeast

   Depositor

   Jorrit Enserink

   Article

   Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

   Insert

   RAP1 (RAP1 Budding Yeast)
   

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   K(43,61,69,117,240,246,527,582,651)R

   Promoter

   GPD

   Available Since

   Dec. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pAG415-GPDp-EGFP-rap1-K651R

   Plasmid

   #84536

   Purpose

   This plasmid can be used to express EGFP-tagged Rap1-K651R from the GDP promoter in yeast

   Depositor

   Jorrit Enserink

   Article

   Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

   Insert

   RAP1 (RAP1 Budding Yeast)
   

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   K651R

   Promoter

   GPD

   Available Since

   Dec. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pSLQ8453 pHR-hU6-LbCpf1 crRNA BB-EF1a-Puro-t2a-BFP-WPRE

   Plasmid

   #140086

   Purpose

   Backbone for cloning U6 driven LbCpf1 (Cas12a) crRNA, EF1a driven BFP + puromycin resistance

   Depositor

   Stanley Qi

   Article

   Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

   Insert

   LbCpf1 (LbCas12a) crRNA backbone
   

   Use

   Lentiviral

   Tags

   EF1a-Puro-T2A-BFP

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. crTet in pSLQ8453

   Plasmid

   #183965

   Purpose

   U6-crTet-EF1a-Puro-T2A-BFP-WPRE

   Depositor

   Stanley Qi

   Article

   Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

   Insert

   crTet
   

   Use

   Lentiviral

   Tags

   EF1a-Puro-T2A-BFP

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLKO.1 GFP shRNA

    Plasmid

    #30323

    Depositor

    David Sabatini

    Article

    Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

    Insert

    GFP shRNA
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    June 2, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLKO.1 puro shRNA beta-catenin

    Plasmid

    #18803

    Purpose

    3rd gen lentiviral vector for knocking down beta-catenin gene expression

    Depositor

    Bob Weinberg

    Article

    Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

    Insert

    b-catenin shRNA (CTNNB1 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 22, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CLP1 gRNA (BRDN0001148453)

    Plasmid

    #76144

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CLP1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    CLP1 (CLP1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLKO.1 puro shRNA E-cadherin

    Plasmid

    #18801

    Depositor

    Bob Weinberg

    Article

    Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

    Insert

    E-cadherin shRNA (CDH1 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 22, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3-myc3-CUL2

    Plasmid

    #19892

    Depositor

    Yue Xiong

    Article

    Ohta et al Mol Cell. 1999 Apr . 3(4):535-41.

    Insert

    cullin 2 (CUL2 Human)
    

    Tags

    myc3

    Expression

    Mammalian

    Available Since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3-DN-hCUL2-FLAG

    Plasmid

    #15819

    Depositor

    Wade Harper

    Article

    Jin et al Methods Enzymol. 2005;399:287-309. doi:

    Insert

    Cullin2 (CUL2 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Dominant negative: N427

    Available Since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLKO.1-sh-hSOX9-1

    Plasmid

    #40644

    Depositor

    Bob Weinberg

    Article

    Guo et al Cell. 2012 Mar 2;148(5):1015-28.

    Insert

    sh-hSOX9-1 (SOX9 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Sept. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLKO.1 Rheb1 shRNA #1

    Plasmid

    #26625

    Depositor

    David Sabatini

    Article

    Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

    Insert

    Rheb1 shRNA (RHEB Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Oct. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pLKO-shBRCA1 #1

    Plasmid

    #44594

    Depositor

    Eros Lazzerini Denchi

    Article

    Okamoto et al Nature. 2013 Feb 6. doi: 10.1038/nature1

    Insert

    BRCA1 (Brca1 Mouse)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pLKO-shBRCA1 #2

    Plasmid

    #44595

    Depositor

    Eros Lazzerini Denchi

    Article

    Okamoto et al Nature. 2013 Feb 6. doi: 10.1038/nature1

    Insert

    BRCA1 (Brca1 Mouse)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only