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Showing: 1 - 20 of 1246 results

  3. pRSET.HaloTag.v7.AviTag

    Plasmid
    #195486
    Purpose
    Bacterial expression plasmid with Halotag v7 insert plus a C-terminus appended AviTag for enzymatic biotinylation.
    Depositor
    Tim Brown, HHMI-JRC Tool Translation Team
    Insert
    HaloTag.v7.AviTag
    Use
    Tags
    6xHIS and AviTag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    195486-plasmid-map-sequence-id-393193

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  8. AviTag-SpyTag003-MBP

    Plasmid
    #184227
    Purpose
    Expresses AviTag and SpyTag003 linked to maltose binding protein in the bacterial cytoplasm. AviTag enables site-specific biotinylation by BirA. SpyTag003 enables reaction with SpyCatcher003.
    Depositor
    Mark Howarth
    Article
    Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:
    Insert
    AviTag-SpyTag003-MBP
    Use
    Tags
    AviTag, His6, SpyTag003, and Thrombin cleavage si…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    184227-plasmid-map-sequence-id-361606

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  9. pCI_mini-mAgrin-AviTag

    Plasmid
    #198131
    Purpose
    Express mini-Agrin in mammalian cells
    Depositor
    Jeffrey Miner
    Insert
    mini-Agrin (Agrn Mouse)
    Use
    Tags
    TEV-Myc-His
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, beta-globin/IgG chimeric intron
    Availability
    Academic Institutions and Nonprofits only
    198131-plasmid-map-sequence-id-393463

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  14. pET28-AviTag-DogTag-MBP

    Plasmid
    #171773
    Purpose
    Expresses AviTag-DogTag-Maltose binding protein in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.
    Depositor
    Mark Howarth
    Article
    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-
    Insert
    AviTag-DogTag-MBP
    Use
    Tags
    His6 and Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    Peptide tag added to N-terminus of MBP via a GSGE…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    171773-plasmid-map-sequence-id-338517

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Showing: 1 - 20 of 1246 results