Showing: 101 - 120 of 7863 results
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Plasmid#165533PurposeDNA Polymerase whose activity is resistant to prolonged incubations at high temperatures (95 degrees C).DepositorInsertTth DNA polymerase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-KTHEAQpol
Plasmid#165542PurposeExonuclease-free, N-terminal deletion mutant of Taq DNA polymerase.DepositorInsertKlenTaq1 polymerase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-Bstpol
Plasmid#165543PurposeFull length DNA polymerase from Bacillus stearothermophilus.DepositorInsertBst DNA Polymerase, Full Length
UseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-BstpolLF
Plasmid#165553PurposeFragment retains 5'-3' polymerase activity from full length Bst DNA Polymerase, while lacking 5'-3' exonuclease activity. Suitable for applications requiring thermophilic strand displacement.DepositorInsertBst DNA Polymerase, Large Fragment
UseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-Pwopol
Plasmid#165562PurposeDNA polymerase; additionally exhibits 3' to 5' exonuclease activity.DepositorInsertPwo DNA Polymerase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-BsupolLF
Plasmid#165563PurposeBsu DNA Polymerase I, Large Fragment retains the 5'-3' polymerase activity of the Bacillus subtilis DNA polymerase I, but lacks the 5'-3' exonuclease domain. This large fragment naturally lacks 3'-5' exonuclease activity. Applications include random primer labeling, second strand cDNA synthesis, single dA tailing, and strand displacement DNA synthesis.DepositorInsertBsu DNA Polymerase I, Large Fragment
UseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-phi29pol
Plasmid#165573PurposeDNA polymerase responsible for protein-primed viral DNA replication by strand displacement with high processivity and fidelity. Possesses three enzymatic activities: DNA synthesis (polymerase), primer terminal protein (TP) deoxynucleotidylation, and 3' to 5' exonuclease activity.DepositorInsertphi29 DNA Polymerase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityIndustry, Academic Institutions, and Nonprofits -
EP-Pol vector
Plasmid#131686PurposeA lentiviral vector that constitutively expresses an error-prone variant of the adenovirus DNA polymeraseDepositorInsertAdenovirus DNA polymerase
UseLentiviralTagsHAExpressionMutationF421Y, D827APromoterAvailabilityAcademic Institutions and Nonprofits only -
FRB-polySH33
Plasmid#175233PurposeBacterial expression and purification, mixed with polyPRM to form condensates, can bind FKBP in the presence of rapamycinDepositorInsertNCK1 (NCK1 Human)
UseTagsExpressionBacterialMutationPromotertacAvailabilityAcademic Institutions and Nonprofits only -
FRB-polySH35
Plasmid#175234PurposeBacterial expression and purification, mixed with polyPRM to form condensates, can bind FKBP in the presence of rapamycinDepositorInsertNCK1 (NCK1 Human)
UseTagsExpressionBacterialMutationPromotertacAvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv2-POLB-KO-g1
Plasmid#176090PurposeCas9 plus POLB gRNA #1; contains a puromycin resistance cassetteDepositorInsertPOLB sgRNA #1 (POLB Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pOlyA(E69A)
Plasmid#132392PurposeMutant version of OlyA that senses sphingomyelin without or with cholesterolDepositorInsertOstreolysin A point mutant (E69A)
UseTagsHis6 tagExpressionBacterialMutationMutations: C62S, E69A, C94S, S151CPromoterAvailabilityAcademic Institutions and Nonprofits only -
POLG-pcDNA3.1
Plasmid#191487PurposeExpresses human POLG gene on pcDNA3.1 backbone. POLG gene is cloned with BamHI and XbaI restriction sites.DepositorInsertPOLG (POLG Human)
UseTagsV5 tag, 6X HisExpressionMammalianMutationPromoterCMV, T7AvailabilityAcademic Institutions and Nonprofits only -
pTol2CG NBT:NRGIIIa:polyA
Plasmid#193012PurposeTol2 transgenic construct that will express human Neuregulin1 Type IIIa in neuronsDepositorInsertsUseTransposon transgenesisTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pTol2CG NBT:NRGIIIa_R551Q:polyA
Plasmid#193013PurposeTol2 transgenic construct that will express human Neuregulin1 Type IIIa R551Q patient variant in neuronsDepositorInsertsUseTransposon transgenesisTagsExpressionMammalianMutationENST00000287842, NM_013956, c.1652G>A, p.(Arg5…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pL0_AC-vapol
Plasmid#202118PurposeLevel 0 plasmid containing the promoter from gene 47434 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.DepositorInsertvapol promoter
UseSynthetic BiologyTagsExpressionMutationNonePromoterAvailabilityIndustry, Academic Institutions, and Nonprofits -
pL0_EF-vapol
Plasmid#202174PurposeLevel 0 plasmid containing a terminator sequence from gene 47434 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.DepositorInsertvapol terminator
UseSynthetic BiologyTagsExpressionMutationNonePromoterAvailabilityIndustry, Academic Institutions, and Nonprofits -
pXPR_023d-sgCiPOLR2D
Plasmid#202445PurposeExpression of a CRISPRi guide targeting POLR2DDepositorInsertPOLR2D gRNA (POLR2D Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6AvailabilityAcademic Institutions and Nonprofits only -
gag-P3-pol
Plasmid#211374PurposePackaging plasmid for v3b PE-eVLP productionDepositorInsertMMLVgag-P3-pol
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
HDR-HaloPOLQ
Plasmid#211637Purposehalo donorDepositorInsertPuro-LoxHaloTag
UseTagsflagExpressionMammalianMutationPromoterSV40AvailabilityAcademic Institutions and Nonprofits only
Showing: 101 - 120 of 7863 results