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Plasmid#85165PurposeProvides the coding region to express the EcoRI restriction enzyme. The RTX tag allows the EcoRI enzyme to be isolated via calcium-precipitation.DepositorInsertEcoRI-RTX and EcoRI Methylase-AmilCP coding sequences
UseTagsAmilCP and RTXExpressionBacterialMutationPromoterJ23100 promoterAvailable sinceDec. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3 EcoRI-RTX with EcoRI Methylase-AmilCP
Plasmid#85165PurposeProvides the coding region to express the EcoRI restriction enzyme. The RTX tag allows the EcoRI enzyme to be isolated via calcium-precipitation.DepositorInsertEcoRI-RTX and EcoRI Methylase-AmilCP coding sequences
UseTagsAmilCP and RTXExpressionBacterialMutationPromoterJ23100 promoterAvailable sinceDec. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOpen-EcoRIR
Plasmid#165504PurposeType II restriction enzyme that recognizes the double-stranded sequence GAATTC and cleaves after G-1.DepositorInsertEcoRI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-EcoRIM
Plasmid#165515PurposeMethylase recognizes double-stranded sequence GAATTC, methylates A-3 on both strands, and protects DNA from cleavage by EcoRI.DepositorInsertM.EcoRI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 29, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
Prham-M.EcoRI-p15A-aadA
Plasmid#149341Purposeexpresses M.EcoRI DNA methyltransferase in E. coliDepositorInsertM.EcoRI
UseTagsExpressionBacterialMutationPromoterrhamnoseAvailable sinceJune 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBlue-hFX-HA(NcoI/EcoRI)
Plasmid#15405DepositorInserthuman frataxin cDNA (FXN Human)
UseTagsAU1 and HAExpressionBacterialMutationinsert can be released by digestion with REs NcoI…PromoterAvailable sinceAug. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3 EcoRI Methylase-AmilCP
Plasmid#85166PurposeFor methylating EcoRI recognition sites in order to prevent cleavage by the EcoRI restriction enzyme. AmilCP is a blue reporter for Methylase expression.DepositorInsertEcoRI Methylase-AmilCP
UseTagsAmilCPExpressionBacterialMutationPromoterJ23100 promoterAvailable sinceDec. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
Prham-M.EcoRI-p15A-aadA
Plasmid#149341Purposeexpresses M.EcoRI DNA methyltransferase in E. coliDepositorInsertM.EcoRI
UseTagsExpressionBacterialMutationPromoterrhamnoseAvailable sinceJune 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBlue-hFX-HA(NcoI/EcoRI)
Plasmid#15405DepositorInserthuman frataxin cDNA (FXN Human)
UseTagsAU1 and HAExpressionBacterialMutationinsert can be released by digestion with REs NcoI…PromoterAvailable sinceAug. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3 EcoRI Methylase-AmilCP
Plasmid#85166PurposeFor methylating EcoRI recognition sites in order to prevent cleavage by the EcoRI restriction enzyme. AmilCP is a blue reporter for Methylase expression.DepositorInsertEcoRI Methylase-AmilCP
UseTagsAmilCPExpressionBacterialMutationPromoterJ23100 promoterAvailable sinceDec. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-MPRA-MluI-SpeI-EcoRI
Plasmid#190196PurposeEmpty vector for inserting MPRA libraries into AAVDepositorTypeEmpty backboneUseAAV; Massively parallel reporter assay (mpra)TagsExpressionMammalianMutationPromoterAvailable sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-retro-puro-shNup35-EcoRI
Plasmid#87332PurposeTo express shRNA against human Nup35DepositorInsertshRNA against human Nup35
UseRNAiTagsExpressionMammalianMutationPromoterH1Available sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-retro-puro-shNup35-EcoRI
Plasmid#87332PurposeTo express shRNA against human Nup35DepositorInsertshRNA against human Nup35
UseRNAiTagsExpressionMammalianMutationPromoterH1Available sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 E2F1 HA(Δ2-126) (HindIII- BamHI- EcoRI)
Plasmid#70668PurposeHuman mutant of E2F1 lacking amino acids 2 to 126DepositorInsertE2F1 lacking residues 2 to 126 (E2F1 Human)
UseTagsHAExpressionMammalianMutationlacks amino acids 2-126PromotercmvAvailable sinceNov. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
Prham-M.XbaI-M.EcoRI-M.SalI-p15A-aadA
Plasmid#170767PurposeMethylase expression vector for 5RM assembly: expresses M.XbaI, M.EcoRI and M.SalI DNA methyltransferases in E. coliDepositorInsertsM.XbaI
M.EcoRI
M.SalI
UseTagsExpressionBacterialMutationPromoterrhamnoseAvailable sinceFeb. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 E2F1 HA(Δ2-126) (HindIII- BamHI- EcoRI)
Plasmid#70668PurposeHuman mutant of E2F1 lacking amino acids 2 to 126DepositorInsertE2F1 lacking residues 2 to 126 (E2F1 Human)
UseTagsHAExpressionMammalianMutationlacks amino acids 2-126PromotercmvAvailable sinceNov. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG>nls-Cas9-nls-2A-Citrine-EcoRI/NotI
Plasmid#169097PurposeCas9 and Citrine expression in transfected cellsDepositorInsertCas9-2A-Citrine
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 E2F1 Flag(Δ193-359) (HindIII- BamHI- EcoRI)
Plasmid#70667PurposeHuman mutant of E2F1 lacking amino acids 193-359DepositorInsertE2F1 lacking residues 193 to 359 (E2F1 Human)
UseTagsFLAGExpressionMammalianMutationlacks amino acids 193-359PromotercmvAvailable sinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 E2F1 Flag(Δ 284−359)(HindIII- BamHI- EcoRI)
Plasmid#70669PurposeHuman mutant of E2F1 lacking amino acids 284 to 359DepositorInsertE2F1 lacking residues 284 to 359 (E2F1 Human)
UseTagsFlagExpressionMammalianMutationlacks amin acids 284 to 359PromotercmvAvailable sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 E2F1 Flag(Δ193-359) (HindIII- BamHI- EcoRI)
Plasmid#70667PurposeHuman mutant of E2F1 lacking amino acids 193-359DepositorInsertE2F1 lacking residues 193 to 359 (E2F1 Human)
UseTagsFLAGExpressionMammalianMutationlacks amino acids 193-359PromotercmvAvailable sinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only